{"title":"在发展准确的测定雌二醇和睾酮在绝经后妇女的挑战。","authors":"Frank Z Stanczyk, Hubert Vesper","doi":"10.1097/GME.0000000000002613","DOIUrl":null,"url":null,"abstract":"<p><p>Circulating concentrations of estradiol (E2) and testosterone (T) have been measured in postmenopausal women for many years, predominantly by immunoassays and mass spectrometry (MS) assays. Although the accuracy of E2 and T assays is very important to provide clinically meaningful results, there are challenges in establishing their accuracy. MS assays demonstrate higher accuracy for steroid hormone measurements, but immunoassays can provide clinically meaningful results, especially at high concentrations. The Centers for Disease Control and Prevention (CDC) has established a program to standardize and improve the measurement of steroid hormones, including E2 and T, using liquid chromatography-tandem MS (LC-MS/MS). In addition, the CDC has partnered to establish postmenopausal reference ranges for T and is developing reference intervals for E2. Minimizing some of the technical limitations of the current immunoassays and MS assays should provide better and more accurate assays for patient care.</p>","PeriodicalId":18435,"journal":{"name":"Menopause: The Journal of The North American Menopause Society","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Challenges in developing accurate assays for the measurement of estradiol and testosterone in postmenopausal women.\",\"authors\":\"Frank Z Stanczyk, Hubert Vesper\",\"doi\":\"10.1097/GME.0000000000002613\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Circulating concentrations of estradiol (E2) and testosterone (T) have been measured in postmenopausal women for many years, predominantly by immunoassays and mass spectrometry (MS) assays. Although the accuracy of E2 and T assays is very important to provide clinically meaningful results, there are challenges in establishing their accuracy. MS assays demonstrate higher accuracy for steroid hormone measurements, but immunoassays can provide clinically meaningful results, especially at high concentrations. The Centers for Disease Control and Prevention (CDC) has established a program to standardize and improve the measurement of steroid hormones, including E2 and T, using liquid chromatography-tandem MS (LC-MS/MS). In addition, the CDC has partnered to establish postmenopausal reference ranges for T and is developing reference intervals for E2. Minimizing some of the technical limitations of the current immunoassays and MS assays should provide better and more accurate assays for patient care.</p>\",\"PeriodicalId\":18435,\"journal\":{\"name\":\"Menopause: The Journal of The North American Menopause Society\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-07-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Menopause: The Journal of The North American Menopause Society\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1097/GME.0000000000002613\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Menopause: The Journal of The North American Menopause Society","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/GME.0000000000002613","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
Challenges in developing accurate assays for the measurement of estradiol and testosterone in postmenopausal women.
Circulating concentrations of estradiol (E2) and testosterone (T) have been measured in postmenopausal women for many years, predominantly by immunoassays and mass spectrometry (MS) assays. Although the accuracy of E2 and T assays is very important to provide clinically meaningful results, there are challenges in establishing their accuracy. MS assays demonstrate higher accuracy for steroid hormone measurements, but immunoassays can provide clinically meaningful results, especially at high concentrations. The Centers for Disease Control and Prevention (CDC) has established a program to standardize and improve the measurement of steroid hormones, including E2 and T, using liquid chromatography-tandem MS (LC-MS/MS). In addition, the CDC has partnered to establish postmenopausal reference ranges for T and is developing reference intervals for E2. Minimizing some of the technical limitations of the current immunoassays and MS assays should provide better and more accurate assays for patient care.
期刊介绍:
Menopause, published monthly, provides a forum for new research, applied basic science, and clinical guidelines on all aspects of menopause. The scope and usefulness of the journal extend beyond gynecology, encompassing many varied biomedical areas, including internal medicine, family practice, medical subspecialties such as cardiology and geriatrics, epidemiology, pathology, sociology, psychology, anthropology, and pharmacology. This forum is essential to help integrate these areas, highlight needs for future research, and enhance health care.