补充柚皮苷可减少大鼠脑缺血时小脑的炎症过程。

IF 3.3 4区 医学 Q3 CHEMISTRY, MEDICINAL
Zubeyde Babacanoglu, Gozde Acar, Tugce Aladag, Saltuk Bugra Baltaci, Rasim Mogulkoc, Abdulkerim Kasim Baltaci
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引用次数: 0

摘要

简介:在脑缺血过程中,脑组织的损伤分为缺血和再灌注两个阶段。在缺血阶段,由于循环系统(脑血管)受损,脑组织经历能量衰竭,导致氧气和葡萄糖剥夺,从而导致脑损伤。目的:研究柚皮苷对实验性局灶性脑缺血再灌注大鼠小脑组织caspase-3、IL-17、NF-κB水平的影响。方法:选用来自Selcuk大学实验动物研究与应用中心的10 ~ 12周龄wistar型大鼠。在全身麻醉下进行大鼠脑缺血再灌注实验(颈动脉缺血30分钟)。试验组组成如下:1)对照组,2)假手术组,3)假手术+载药组,4)缺血再灌注组,5)缺血再灌注+柚皮苷补充组,给药2周(100mg/kg)。实验结束时,测定全麻大鼠小脑组织中IL-17、caspase-3、NF-κB水平。首先,从心脏抽血,动物被颈椎脱臼杀死。结果:实验性脑缺血再灌注后大鼠脑组织caspase-3、IL-17、NF-κB水平显著升高。相反,补充柚皮苷2周可显著抑制缺血再灌注诱导的炎症过程。讨论:我们的研究结果普遍表明,由于大鼠局灶性脑缺血再灌注,参与炎症通路的关键分子NF-κB以及促炎细胞因子IL-17和凋亡指标caspase-3在小脑组织中的水平显著升高。然而,缺血再灌注后两周灌胃柚皮苷可显著改善缺血损伤引起的不良反应。结论:柚皮苷能有效减轻大鼠脑缺血再灌注后小脑的炎症活化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Naringin Supplementation Reduces Inflammatory Processes in the Cerebellum in Brain Ischemia of Rats.

Introduction: During cerebral ischemia, brain tissue is damaged in two successive stages: ischemia and reperfusion (I/R). In the ischemic phase, brain tissue undergoes energy failure due to an impaired circulatory system (cerebrovascular), resulting in oxygen and glucose deprivation and consequent brain damage.

Objective: The study aimed to determine the effect of a two-week administration of naringin on caspase-3, IL-17, and NF-κB levels in cerebellar tissue in experimental focal brain ischemiareperfusion in rats.

Methods: The research was conducted on 10- to 12-week-old Wistar-type rats obtained from the Selcuk University Experimental Animals Research and Application Center. Experimental brain ischemia-reperfusion in rats was performed under general anesthesia (carotid arteries were exposed to ischemia for 30 minutes). Experimental groups were formed as follows. 1) Control group, 2) Sham, 3) Sham + vehicle, 4) Ischemia-reperfusion, 5) Ischemia-reperfusion + Naringin supplemented group for two weeks (100mg/kg). At the end of the experiments, the levels of IL-17, caspase-3, and NF-κB were determined in the cerebellum tissue of the animals under general anesthesia. First of all, blood was drawn from the heart, and the animals were killed by cervical dislocation.

Results: Experimental brain ischemia-reperfusion significantly increased caspase-3, IL-17, and NF-κB levels in the brain tissue of rats. In contrast, naringin supplementation for 2 weeks significantly suppressed the ischemia-reperfusion-induced inflammatory process.

Discussion: The findings obtained from our research generally showed that, as a result of focal brain ischemia-reperfusion in rats, the levels of NF-κB, a key molecule involved in inflammatory pathways, as well as the pro-inflammatory cytokine IL-17 and caspase-3, an indicator of apoptosis, increased significantly in cerebellar tissue. However, intragastric naringin supplementation for two weeks following ischemia-reperfusion led to significant improvements in the adverse effects caused by the ischemic injury.

Conclusion: The study's results demonstrate that naringin treatment effectively mitigates inflammatory activation in the cerebellum following brain ischemia-reperfusion in rats.

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来源期刊
CiteScore
6.40
自引率
2.90%
发文量
186
审稿时长
3-8 weeks
期刊介绍: Current Topics in Medicinal Chemistry is a forum for the review of areas of keen and topical interest to medicinal chemists and others in the allied disciplines. Each issue is solely devoted to a specific topic, containing six to nine reviews, which provide the reader a comprehensive survey of that area. A Guest Editor who is an expert in the topic under review, will assemble each issue. The scope of Current Topics in Medicinal Chemistry will cover all areas of medicinal chemistry, including current developments in rational drug design, synthetic chemistry, bioorganic chemistry, high-throughput screening, combinatorial chemistry, compound diversity measurements, drug absorption, drug distribution, metabolism, new and emerging drug targets, natural products, pharmacogenomics, and structure-activity relationships. Medicinal chemistry is a rapidly maturing discipline. The study of how structure and function are related is absolutely essential to understanding the molecular basis of life. Current Topics in Medicinal Chemistry aims to contribute to the growth of scientific knowledge and insight, and facilitate the discovery and development of new therapeutic agents to treat debilitating human disorders. The journal is essential for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important advances.
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