白藜芦醇通过脑源性神经营养因子相关的结构突触重塑改善小鼠卵巢切除术和慢性约束应激诱导的抑郁样行为。

IF 1.6 4区 心理学 Q3 BEHAVIORAL SCIENCES
Behavioural Pharmacology Pub Date : 2025-09-01 Epub Date: 2025-07-31 DOI:10.1097/FBP.0000000000000845
Hui Xu, Zhen-Qiang Zhang, Geng Chen, Ming-Jun Ge, Zong-Hao Yu, Jun-Xian Shen, Chuan Pan, Fei Han, Xiu-Ling Zhu, Ya-Ping Lu
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引用次数: 0

摘要

既往研究表明,白藜芦醇在多种抑郁症模型中均具有抗抑郁作用,但白藜芦醇对绝经期抑郁症的作用及机制尚不清楚。本研究采用转基因小鼠去卵巢联合慢性约束应激建立绝经期抑郁模型。采用悬尾试验(TST)、强迫游泳试验、蔗糖偏好试验(SPT)和新型抑制喂养试验(NSFT)评价白藜芦醇的抗抑郁作用。利用黄色荧光蛋白在转基因小鼠兴奋性神经元中的特征性表达,利用三维成像技术评价白藜芦醇对小鼠树突和树突棘密度的影响。采用定量PCR和免疫荧光定量分析脑源性神经营养因子(BDNF)、cofilin1和p-cofilin1,探讨白藜芦醇对海马和内侧前额叶皮质(mPFC)突触可塑性的影响及其机制。结果表明,白藜芦醇显著降低了TST期的静止时间,缩短了NSFT期的摄食潜伏期,增加了NSFT期的摄食量,增加了SPT期的蔗糖消耗。与这些变化一致,白藜芦醇处理显著增加了这些脑区p-cofilin1免疫反应性树突棘的密度和BDNF的mRNA水平。结果提示,白藜芦醇可通过上调BDNF水平,增强cofilin 1磷酸化,增加海马和mPFC的树突和树突棘密度,改善相应脑区突触可塑性,最终改善绝经期抑郁样行为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Resveratrol improves ovariectomy and chronic restraint stress-induced depression-like behaviors in mice through brain-derived neurotrophic factor associated structural synaptic remodeling.

Previous studies have shown that resveratrol has antidepressant effects in a variety of depression models, but the effect and mechanism of resveratrol on menopausal depression are unclear. In this study, transgenic mice were ovariectomized combined with chronic restraint stress to establish a model of menopausal depression. The antidepressant effect of resveratrol was evaluated by tail suspension test (TST), forced swimming test, sucrose preference test (SPT), and novel inhibition feeding test (NSFT). Using the characteristic expression of yellow fluorescent protein in excitatory neurons of transgenic mice, the effects of resveratrol on the density of dendrites and dendritic spines were evaluated by a three-dimensional imaging technique. Brain-derived neurotrophic factor (BDNF), cofilin1, and p-cofilin1 were quantitatively analyzed by quantitative PCR and immunofluorescence quantification to explore the effects of resveratrol on synaptic plasticity in the hippocampus and medial prefrontal cortex (mPFC) and its mechanism. The results revealed that resveratrol significantly decreased the immobility time in TST, shortened the feeding latency and increased the food intake in NSFT, and enhanced the sucrose consumption in SPT. Consistent with these changes, resveratrol treatment significantly increased the density of p-cofilin1 immunoreactive dendritic spines and the mRNA level of BDNF in these brain regions. The results suggest that resveratrol can improve the synaptic plasticity in the corresponding brain regions by upregulating BDNF levels, enhancing the phosphorylation of cofilin 1, increasing the density of dendrites and dendritic spines in the hippocampus and mPFC, and ultimately improving menopausal depression-like behaviors.

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来源期刊
Behavioural Pharmacology
Behavioural Pharmacology 医学-行为科学
CiteScore
3.40
自引率
0.00%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Behavioural Pharmacology accepts original full and short research reports in diverse areas ranging from ethopharmacology to the pharmacology of schedule-controlled operant behaviour, provided that their primary focus is behavioural. Suitable topics include drug, chemical and hormonal effects on behaviour, the neurochemical mechanisms under-lying behaviour, and behavioural methods for the study of drug action. Both animal and human studies are welcome; however, studies reporting neurochemical data should have a predominantly behavioural focus, and human studies should not consist exclusively of clinical trials or case reports. Preference is given to studies that demonstrate and develop the potential of behavioural methods, and to papers reporting findings of direct relevance to clinical problems. Papers making a significant theoretical contribution are particularly welcome and, where possible and merited, space is made available for authors to explore fully the theoretical implications of their findings. Reviews of an area of the literature or at an appropriate stage in the development of an author’s own work are welcome. Commentaries in areas of current interest are also considered for publication, as are Reviews and Commentaries in areas outside behavioural pharmacology, but of importance and interest to behavioural pharmacologists. Behavioural Pharmacology publishes frequent Special Issues on current hot topics. The editors welcome correspondence about whether a paper in preparation might be suitable for inclusion in a Special Issue.
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