从鲣鱼皮肤废料中提取的胶原蛋白增强了纳米纤维和水凝胶支架的细胞增殖活性、血管化潜力和抗炎性能

IF 4.9
Tejaswini Petkar, Marie Andrea Laetitia Huët, Devesh Bekah, Itisha Chummun Phul, Nowsheen Goonoo and Archana Bhaw-Luximon
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引用次数: 0

摘要

海洋胶原蛋白作为一种可持续的生物材料和哺乳动物胶原蛋白的安全替代品,在组织工程中越来越受到重视。采用醋酸提取法从鲣鱼皮废中提取胶原蛋白,得率为10.02±2.69%。SDS - PAGE显示存在分子量为120-140 kDa的α-胶原链(α1和α2),在~ 200 kDa处有β二聚体,在~ 250 kDa处有γ三聚体。所提取的胶原中羟脯氨酸含量(14.42±0.11%)高于文献报道值,具有较好的结构完整性和热稳定性。将提取的胶原加入到三种支架中,即聚二氧环酮/聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PDX/PHBV 50/50, 10 wt%)纳米纤维垫、聚甲基丙烯酸蔗糖水凝胶(PSucMA 5 wt%)和纤维素-木质素水凝胶(Cel-lig 2 wt%)。体外实验采用RAW 264.7巨噬细胞评估炎症活性,人真皮成纤维细胞(HDFs)评估增殖活性,人脐静脉内皮细胞(HUVECs)评估胶原支架的血管化潜力。巨噬细胞在胶原存在下炎症M1表型降低,而HUVECs和HDFs增殖增强。总的来说,鱼皮废弃胶原蛋白具有提高性能的潜力,并允许多任务支架的工程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Collagen from skipjack tuna skin waste enhances cellular proliferative activity, vascularization potential and anti-inflammatory properties of nanofibrous and hydrogel scaffolds†

Collagen from skipjack tuna skin waste enhances cellular proliferative activity, vascularization potential and anti-inflammatory properties of nanofibrous and hydrogel scaffolds†

Marine collagen is gaining prominence in tissue engineering as a sustainable biomaterial and a safe alternative to mammalian collagen. Collagen was extracted from skipjack tuna skin waste using the acetic acid extraction method with a yield of 10.02 ± 2.69%. SDS PAGE indicated the presence of α-tropocollagen chains (α1 and α2) with molecular weights of 120–140 kDa, a β dimer at ∼200 kDa, and a γ component trimer at ∼250 kDa. The hydroxyproline content of the extracted collagen (14.42 ± 0.11%) was higher than reported values, indicating better structural integrity and thermostability. The extracted collagen was added to three scaffolds namely a polydioxanone/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PDX/PHBV 50/50, 10 wt%) nanofibrous mat, polysucrose methacrylate hydrogel (PSucMA 5 wt%) and cellulose-lignin (Cel-lig 2 wt%) hydrogel. In vitro experiments were performed using RAW 264.7 macrophages to assess inflammatory activity, human dermal fibroblasts (HDFs) to assess proliferative activity and Human Umbilical Vein Endothelial Cells (HUVECs) to assess the vascularization potential of the scaffolds with collagen. The macrophages showed a reduced inflammatory M1 phenotype in the presence of collagen, while HUVECs and HDFs showed enhanced proliferation. Overall, fish skin waste collagen has the potential to enhance the performance and allows the engineering of multitasking scaffolds.

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