平果无毛（DC.）Guill。&穗青葱。提取物保护大鼠抗ccl4诱导的肝纤维化

IF 1.9 Q2 MEDICINE, GENERAL & INTERNAL

Journal of Taibah University Medical Sciences Pub Date : 2025-08-01 DOI:10.1016/j.jtumed.2025.07.003

Omokolade O. Alejolowo MSc , Olarewaju M. Oluba PhD , Oluyomi S. Adeyemi PhD

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引用次数: 0

摘要

目的：探讨平ocarpus （DC.）Guill。,穗青葱。在尼日利亚当地用于治疗肝功能障碍。本研究探讨了白菖蒲茎皮氢甲醇提取物（AL）对四氯化碳（CCl4）诱导的Wistar大鼠肝纤维化的保护作用。方法35只雄性大鼠随机分为7组。组1（对照组）给予蒸馏水和橄榄油，组2（阴性对照组）每周3次腹腔注射含30% CCl4的橄榄油，持续8周。除CCl4外，III-VI组大鼠口服水飞蓟素（阳性对照）和分级剂量（125、250和500 mg/kg体重）的AL。VII组（随访组）只给予CCl4，但在尸检前再保留2周。8周后，解剖动物，进行各种生化、组织学和免疫组织化学评估。结果高效液相色谱法检测到没食子酸、castalagin等13种化合物。与对照组相比，CCl4暴露导致肝脏指数显著增加，但AL给药后肝脏指数有所改善。AL升高抗氧化参数，包括超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽过氧化物酶水平，丙二醛水平相应下降。此外，与CCl4组相比，AL引起血浆肿瘤坏死因子α和白细胞介素6水平下降。与CCl4相比，AL治疗可适度减轻肝细胞囊化，同时降低肝α-平滑肌肌动蛋白（α-SMA）和转化生长因子β (TGF-β)的表达。上述结果提示，AL的抗纤维化作用可能与下调α-SMA和TGF-β的表达有关。此外，与对照组和CCl4 +水飞蓟素治疗组相比，AL明显改善了肝功能指标（谷丙转氨酶、天冬氨酸转氨酶和碱性磷酸酶）。综上所述，AL具有抗炎、保肝、抗纤维化的作用，作为肝脏疾病的潜在治疗药物具有进一步探索的前景。

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Anogeissus leiocarpus (DC.) Guill. & Perr. extract-protected rats against CCl4-induced liver fibrosis

Objective

Anogeissus leiocarpus (DC.) Guill. & Perr. is locally used for the treatment of hepatic dysfunctions in Nigeria. This study investigated the hepatoprotective impact of A. leiocarpus stem bark hydromethanolic extract (AL) in carbon tetrachloride (CCl₄)-induced hepatic fibrosis in Wistar rats.

Methods

Thirty-five male rats were randomly assigned to seven groups. Group I (control) received distilled water and olive oil, and Group II (negative control) received 30 % CCl₄ intraperitoneally in olive oil three times a week for 8 weeks. In addition to CCl₄, rats in groups III–VI were orally given silymarin (positive control) and graded doses (125, 250, and 500 mg/kg body weight) of AL. Group VII (follow-up group) received CCl₄ only, but was spared for another 2 weeks before necropsy. After 8 weeks, the animals were necropsied, and various biochemical, histological, and immunohistochemical assessments were conducted.

Results

High-performance liquid chromatography analysis of AL detected 13 compounds, including gallic acid and castalagin. CCl₄ exposure led to a significant increase in liver index relative to the control, but the increased liver index improved following AL administration. AL elevated the antioxidant parameters, including the levels of superoxide dismutase, glutathione peroxidase, and glutathione peroxidase, with a corresponding decline in malondialdehyde levels. Furthermore, AL caused a decline in the plasma level of tumor necrosis factor alpha and interleukin 6 relative to the CCl₄ group. AL treatment moderately thinned the hepatocyte ballooning, while reducing the expression of hepatic alpha smooth muscle actin (α-SMA) and transforming growth factor beta (TGF-β) compared with CCl₄. These findings indicate that the antifibrotic action of AL might involve the downregulation of α-SMA and TGF-β expression. Furthermore, AL appreciably improved liver function indices (alanine transaminase, aspartate transaminase, and alkaline phosphatase) in a manner comparable to that of the control and CCl₄ + silymarin treatment groups.

Conclusion

Collectively, AL exhibited anti-inflammatory, hepatoprotective, and antifibrotic effects and thus has prospects for further exploration as a potential therapy in liver diseases.

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