兔声门下粘膜病变的光学频域成像与病理一致性的临床前评估

IF 1.3 4区 医学 Q3 OTORHINOLARYNGOLOGY
Yuki Komyo , Yuichiro Tomioka , Yuichi Okata , Harunori Miyauchi , Taichi Nakatani , Shino Inuzuka , Tomoo Itoh , Yuko Bitoh
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引用次数: 0

摘要

目的小儿声门下气道易发生先天性和后天性病变。声门下粘膜病变的准确成像对于适当的治疗是必不可少的,但现有的方法,如支气管镜检查或计算机断层扫描(CT)往往缺乏分辨率或客观性。光学相干断层扫描(OCT)已被使用,但基于OCT的成像与组织病理学结果之间的相关性尚未得到评估。光学频域成像(OFDI)是OCT的临床级变体,提供更高的分辨率和更快的采集。我们的目的是评估OFDI是否可以安全、清晰地显示声门下粘膜结构,并检查在动物模型中的成像结果与组织学特征的对应程度。方法选用体重约3 kg的家兔12只。在麻醉下,刷刷擦洗以诱导声门下间隙粘膜病变。对切除的喉部进行病理检查。主要疗效终点是声门下间隙的周向可视化,以及在区分软骨、粘膜和病变方面与病理的一致性。主要的安全终点是OFDI期间没有粘膜损伤和导管移位。结果声门下间隙图像清晰,OFDI清晰地区分了软骨、粘膜和病变。OCT评估的观察者间信度为0.87,OCT与组织学评估的一致性为0.94。未见粘膜出血或导管引起的破裂。结论OFDI可以安全使用,其诊断价值与声门下间隙粘膜病变的组织学评估相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preclinical assessment of agreement between optical frequency domain imaging and pathology in rabbit subglottic mucosal lesions

Objective

The pediatric subglottic airway is susceptible to various pathological conditions, both congenital and acquired. Accurate imaging of subglottic mucosal lesions is essential for appropriate treatments, but existing methods such as bronchoscopy or computed tomography (CT) often lack resolution or objectivity. Optical coherence tomography (OCT) has been used, but the correlation between OCT-based imaging and histopathological findings has not been evaluated. Optical frequency domain imaging (OFDI), a clinical-grade variant of OCT, offers higher resolution and faster acquisition. We aimed to evaluate whether OFDI can safely and clearly visualize subglottic mucosal structures and examine how well the imaging findings correspond to histological features in an animal model.

Methods

Twelve rabbits weighing approximately 3 kg were used. Under anesthesia, brush scrubbing was performed to induce mucosal lesions in the subglottic space. The resected larynx was pathologically examined. The primary efficacy endpoints were circumferential visualization of the subglottic space and concordance with pathology in differentiating between the cartilage, mucosa, and lesions. The primary safety endpoints were the absence of mucosal injury and catheter dislodgement during OFDI.

Results

Clear images of the subglottic space were obtained, and OFDI clearly distinguished the cartilage from the mucosa and lesions. The interobserver reliability of the OCT assessment was 0.87, and the agreement between OCT and the histological assessment was 0.94. No mucosal hemorrhage or catheterization-induced dehiscence was observed.

Conclusions

Our findings suggest that OFDI can be used safely and has a diagnostic performance comparable to that of histological assessment of mucosal lesions in the subglottic space.
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来源期刊
CiteScore
3.20
自引率
6.70%
发文量
276
审稿时长
62 days
期刊介绍: The purpose of the International Journal of Pediatric Otorhinolaryngology is to concentrate and disseminate information concerning prevention, cure and care of otorhinolaryngological disorders in infants and children due to developmental, degenerative, infectious, neoplastic, traumatic, social, psychiatric and economic causes. The Journal provides a medium for clinical and basic contributions in all of the areas of pediatric otorhinolaryngology. This includes medical and surgical otology, bronchoesophagology, laryngology, rhinology, diseases of the head and neck, and disorders of communication, including voice, speech and language disorders.
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