Nonsubstrate PI(4,5)P 2 interacts with the interdomain linker to control electrochemical coupling in voltage-sensing phosphatase (VSP)

Voltage-sensing phosphatase (VSP) comprises a voltage sensor domain (VSD) and a cytoplasmic catalytic region (CCR), achieving a unique electrochemical signal conversion. Previous studies suggest that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P 2 ), a membrane phospholipid known to be critical for activities of diverse voltage-gated ion channels, associates with a linker connecting the VSD with the CCR of VSP and regulates VSD-CCR coupling. However, the details of PI(4,5)P 2 interaction with the linker of VSP remain elusive. Here, we exploit advantage of sensitivity of a fluorescent unnatural amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), to changes in local environment to study interaction between PI(4,5)P 2 and the linker of Ciona intestinalis VSP (Ci-VSP). We found that a conserved tyrosine residue (Y255) as well as neighboring basic residues interacts with PI(4,5)P 2 and this interaction was maintained in G365A Ci-VSP mutant which lacks the substrate PI(4,5)P 2 at the active site and Ci-VSP/human phosphatase and tensin homolog (PTEN) chimera which does not dephosphorylate PI(4,5)P 2 , indicating that the linker interacts with nonsubstrate, regulatory PI(4,5)P 2 outside the active site. Molecular dynamics simulations demonstrated that the linker formed stable interaction with PI(4,5)P 2 in the activated state. These findings indicate that regulation of coupling to an effector region downstream of the VSD through PI(4,5)P 2 binding to the linker is shared among voltage-dependent membrane proteins.