Alma Gisbert Iranzo, Marina Cano-Extremera, Irene Hervás, Mar Falquet Guillem, María Gil Juliá, Ana Navarro-Gomezlechon, Rosa María Pacheco-Rendón, Nicolás Garrido
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The analysis revealed that sperm selection using microfluidics yields lower sperm DNA fragmentation (MD = -9.98 [-13.19, -6.76], <i>p</i> < 0.00001), increased progressive motility (MD = 14.50 [7.84, 21.71], <i>p</i> = 0.04), total motility (MD = 10.68 [6.04, 15.31], <i>p</i> < 0.00001) and morphology (MD = 1.41 [0.67, 2.16], <i>p</i> = 0.0002). Significant differences were also found in the fertilization rate/MII oocyte microinjected (OR = 1.22 [1.01, 1.46], <i>p</i> = 0.04), implantation rate/embryo transfer (ET) (OR = 4.51 [1.42, 14.37], <i>p</i> = 0.01), clinical pregnancy/ET (OR = 1.73 [1.22, 2.45], <i>p</i> = 0.002), ongoing pregnancy/ET (OR = 1.99 [1.03, 3.83], <i>p</i> = 0.04), live birth rate/first cycle (OR = 1.59 [1.12, 2.24], <i>p</i> = 0.009) and per all embryo transfer (OR = 1.65 [1.06, 2.55], <i>p</i> = 0.03). No significant differences were found in embryo euploidy/number of biopsied blastocysts (OR = 1.34 [0.88, 2.04], <i>p</i> = 0.77), biochemical pregnancy/ET (OR = 1.23 [0.84, 1.80], <i>p</i> = 0.29), miscarriage rate/cycle (OR = 0.84 [0.54, 1.31], <i>p</i> = 0.35) and per pregnancy (OR = 0.71 [0.50, 1.02], <i>p</i> = 0.07), live birth rate/first embryo transfer (OR = 1.60 [0.80, 3.22], <i>p</i> = 0.18) and per concluded cycle (OR = 1.03 [0.53, 2.00], <i>p</i> = 0.92). To summarize, microfluidics may offer a beneficial approach in certain situations, particularly for patients with elevated sperm DNA fragmentation (SDF) levels. However, its integration into routine clinical practice cannot be justified yet in terms of cost-effectiveness. Additional research is needed to provide more comprehensive data on reproductive outcomes, especially live birth rates, which remain the ultimate goal of assisted reproductive technologies.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sperm Selection Using Microfluidic Techniques Significantly Decreases Sperm DNA Fragmentation (SDF), Enhancing Reproductive Outcomes: A Systematic Review and Meta-Analysis.\",\"authors\":\"Alma Gisbert Iranzo, Marina Cano-Extremera, Irene Hervás, Mar Falquet Guillem, María Gil Juliá, Ana Navarro-Gomezlechon, Rosa María Pacheco-Rendón, Nicolás Garrido\",\"doi\":\"10.3390/biology14070792\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to compare sperm parameters and reproductive outcomes after sperm selection using microfluidic chips versus conventional techniques (swim-up/density gradients). A systematic review and meta-analysis were performed after the extraction of relevant data from thirty-nine studies that met the inclusion criteria. Mean difference or odds ratio was calculated for each outcome. The analysis revealed that sperm selection using microfluidics yields lower sperm DNA fragmentation (MD = -9.98 [-13.19, -6.76], <i>p</i> < 0.00001), increased progressive motility (MD = 14.50 [7.84, 21.71], <i>p</i> = 0.04), total motility (MD = 10.68 [6.04, 15.31], <i>p</i> < 0.00001) and morphology (MD = 1.41 [0.67, 2.16], <i>p</i> = 0.0002). Significant differences were also found in the fertilization rate/MII oocyte microinjected (OR = 1.22 [1.01, 1.46], <i>p</i> = 0.04), implantation rate/embryo transfer (ET) (OR = 4.51 [1.42, 14.37], <i>p</i> = 0.01), clinical pregnancy/ET (OR = 1.73 [1.22, 2.45], <i>p</i> = 0.002), ongoing pregnancy/ET (OR = 1.99 [1.03, 3.83], <i>p</i> = 0.04), live birth rate/first cycle (OR = 1.59 [1.12, 2.24], <i>p</i> = 0.009) and per all embryo transfer (OR = 1.65 [1.06, 2.55], <i>p</i> = 0.03). No significant differences were found in embryo euploidy/number of biopsied blastocysts (OR = 1.34 [0.88, 2.04], <i>p</i> = 0.77), biochemical pregnancy/ET (OR = 1.23 [0.84, 1.80], <i>p</i> = 0.29), miscarriage rate/cycle (OR = 0.84 [0.54, 1.31], <i>p</i> = 0.35) and per pregnancy (OR = 0.71 [0.50, 1.02], <i>p</i> = 0.07), live birth rate/first embryo transfer (OR = 1.60 [0.80, 3.22], <i>p</i> = 0.18) and per concluded cycle (OR = 1.03 [0.53, 2.00], <i>p</i> = 0.92). 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引用次数: 0
摘要
本研究旨在比较使用微流控芯片和传统技术（游动/密度梯度）选择精子后的精子参数和生殖结果。从符合纳入标准的39项研究中提取相关数据后进行系统评价和荟萃分析。计算每个结果的平均差异或优势比。分析显示，采用微流体技术进行精子选择，精子DNA碎片化程度降低（MD = -9.98 [-13.19, -6.76], p < 0.00001），进进运动能力（MD = 14.50 [7.84, 21.71], p = 0.04），总运动能力（MD = 10.68 [6.04, 15.31], p < 0.00001）和形态（MD = 1.41 [0.67, 2.16], p = 0.0002）。受精率/MII卵母细胞微注射（OR = 1.22 [1.01, 1.46], p = 0.04）、着床率/胚胎移植（OR = 4.51 [1.42, 14.37], p = 0.01）、临床妊娠/ET （OR = 1.73 [1.22, 2.45], p = 0.002）、持续妊娠/ET （OR = 1.99 [1.03, 3.83], p = 0.04）、活产率/第一周期（OR = 1.59 [1.12, 2.24], p = 0.009）、胚胎移植率（OR = 1.65 [1.06, 2.55], p = 0.03）均存在显著差异。胚胎整倍性/活检囊胚数（OR = 1.34 [0.88, 2.04], p = 0.77）、生化妊娠/ET （OR = 1.23 [0.84, 1.80], p = 0.29）、流产率/周期（OR = 0.84 [0.54, 1.31], p = 0.35）、单次妊娠（OR = 0.71 [0.50, 1.02], p = 0.07）、活产率/首次胚胎移植（OR = 1.60 [0.80, 3.22], p = 0.18）和单次妊娠结束周期（OR = 1.03 [0.53, 2.00], p = 0.92）均无显著差异。总之，微流体技术在某些情况下可能是一种有益的方法，特别是对于精子DNA片段化（SDF）水平升高的患者。然而，从成本效益的角度来看，将其纳入常规临床实践尚不合理。需要进行更多的研究，以提供关于生殖结果的更全面的数据，特别是活产率，这仍然是辅助生殖技术的最终目标。
Sperm Selection Using Microfluidic Techniques Significantly Decreases Sperm DNA Fragmentation (SDF), Enhancing Reproductive Outcomes: A Systematic Review and Meta-Analysis.
This study aimed to compare sperm parameters and reproductive outcomes after sperm selection using microfluidic chips versus conventional techniques (swim-up/density gradients). A systematic review and meta-analysis were performed after the extraction of relevant data from thirty-nine studies that met the inclusion criteria. Mean difference or odds ratio was calculated for each outcome. The analysis revealed that sperm selection using microfluidics yields lower sperm DNA fragmentation (MD = -9.98 [-13.19, -6.76], p < 0.00001), increased progressive motility (MD = 14.50 [7.84, 21.71], p = 0.04), total motility (MD = 10.68 [6.04, 15.31], p < 0.00001) and morphology (MD = 1.41 [0.67, 2.16], p = 0.0002). Significant differences were also found in the fertilization rate/MII oocyte microinjected (OR = 1.22 [1.01, 1.46], p = 0.04), implantation rate/embryo transfer (ET) (OR = 4.51 [1.42, 14.37], p = 0.01), clinical pregnancy/ET (OR = 1.73 [1.22, 2.45], p = 0.002), ongoing pregnancy/ET (OR = 1.99 [1.03, 3.83], p = 0.04), live birth rate/first cycle (OR = 1.59 [1.12, 2.24], p = 0.009) and per all embryo transfer (OR = 1.65 [1.06, 2.55], p = 0.03). No significant differences were found in embryo euploidy/number of biopsied blastocysts (OR = 1.34 [0.88, 2.04], p = 0.77), biochemical pregnancy/ET (OR = 1.23 [0.84, 1.80], p = 0.29), miscarriage rate/cycle (OR = 0.84 [0.54, 1.31], p = 0.35) and per pregnancy (OR = 0.71 [0.50, 1.02], p = 0.07), live birth rate/first embryo transfer (OR = 1.60 [0.80, 3.22], p = 0.18) and per concluded cycle (OR = 1.03 [0.53, 2.00], p = 0.92). To summarize, microfluidics may offer a beneficial approach in certain situations, particularly for patients with elevated sperm DNA fragmentation (SDF) levels. However, its integration into routine clinical practice cannot be justified yet in terms of cost-effectiveness. Additional research is needed to provide more comprehensive data on reproductive outcomes, especially live birth rates, which remain the ultimate goal of assisted reproductive technologies.
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Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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