芍药根提取物配方可诱导子宫内膜异位症体外受精后子宫内膜病变萎缩,加速胚胎着床的实验研究。

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
Amir Abdolmaleki, Hadis Amirsayyafi, Saeed Khazaiel, Cyrus Jalili, Kamran Mansourhi, Mitra Bakhtiari
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引用次数: 0

摘要

目的:子宫内膜异位症(Endo)涉及病变内的炎症和血管生成,可能导致胚胎着床失败。芍药根具有抗血管生成和抗炎作用。本实验探讨了丙二醇对体外受精后子宫内膜异位症病变萎缩及胚胎着床的治疗作用。方法:将32只雌性小鼠分为治疗组和假手术组。通过异种移植大鼠子宫内膜到受体(远藤)小鼠前腹壁诱导远藤。提取荷兰根,对其进行植物化学表征,并连续17天口服(1.06 mg PL/20 g小鼠)。通过体外受精,将培养的小鼠胚胎植入远藤小鼠子宫。体外受精10天后,收集标本,包括腹腔内液,采用酶联免疫吸附法测定血管内皮生长因子(VEGF)和肿瘤坏死因子α (TNF-α)。含胚子宫行台盼蓝染色,子宫碎片行苏木精和伊红染色,定量聚合酶链反应分析白血病抑制因子(LIF)基因表达。记录胚胎着床部位数和子宫内膜异位症病变直径。数据采用SPSS ver进行分析。结果:Endo诱导后,肿瘤坏死因子(TNF-α)和血管内皮生长因子(VEGF)水平升高,病变直径增大(p)。结论:白藜芦醇提取物(1.06 mg/20 g小鼠)可降低肿瘤坏死因子(TNF-α)和血管内皮生长因子(VEGF)水平,抑制炎症和血管生成,引起子宫内膜异位症病变萎缩。此外,PL增加子宫LIF基因表达,促进远藤小鼠体外受精后成功着床。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Formulation of Paeonia lactiflora root extract can induce atrophy of endometriotic lesions and accelerate embryo implantation following in vitro fertilization in endometriosis: An experimental study.

Objective: Endometriosis (Endo) involves inflammation and angiogenesis within lesions, potentially causing embryo implantation failure. Paeonia lactiflora (PL) root exhibits anti-angiogenic and anti-inflammatory properties. This experiment investigated the therapeutic effects of PL on endometriotic lesion atrophy and embryo implantation following in vitro fertilization (IVF).

Methods: Female mice (n=32) were allocated into treatment and sham groups. Endo was induced through xenograft transplantation of rat endometrium to anterior abdominal walls of recipient (Endo) mice. PL root was extracted, phytochemically characterized, and orally administered (1.06 mg PL/20 g mouse) for 17 consecutive days. Through IVF, cultured mouse embryos were implanted into Endo mouse uteri. Ten days post-IVF, samples were collected, including intra-abdominal fluid for measurement of vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α) using enzyme-linked immunosorbent assay. Embryo-containing uteri underwent trypan blue staining, while uterus fragments were stained with hematoxylin and eosin and analyzed for leukemia inhibitory factor (LIF) gene expression using quantitative polymerase chain reaction. The number of embryo implantation sites and diameter of endometriotic lesions were recorded. Data were analyzed using SPSS ver. 19, with p-values <0.05 considered to indicate statistical significance.

Results: Following Endo induction, TNF-α and VEGF levels and lesion diameter increased (p<0.05). LIF gene expression and embryo implantation rate decreased (p<0.05). After PL extract administration to Endo mice, TNF-α levels, VEGF levels, and lesion diameter decreased (p<0.05), while LIF gene expression and implantation rate increased (p<0.05).

Conclusion: PL extract (1.06 mg/20 g mouse) decreases TNF-α and VEGF levels, suppressing inflammation and angiogenesis and causing endometriotic lesion atrophy. Furthermore, PL increases uterine LIF gene expression, promoting successful implantation post-IVF in Endo mice.

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