Jing Ji, Runzhong Wang, Baoying Ma, Tiange Li, Chunfeng Guan
{"title":"Sphingobacterium sp. PNL1与肌醇联合施用，通过促进植物生长、降低氧化胁迫、提高光合效率和降解土壤中的BDE-209，增强了BDE-209污染土壤的修复能力。","authors":"Jing Ji, Runzhong Wang, Baoying Ma, Tiange Li, Chunfeng Guan","doi":"10.1007/s11274-025-04500-w","DOIUrl":null,"url":null,"abstract":"<p><p>As a polybrominated diphenyl ether (PBDE) flame retardant, BDE-209 has attracted attention because of its extensive accumulation in soil and toxic effect on plant growth. Plant promoting rhizosphere bacteria (PGPR) assisted phytoremediation is widely recognized for removing contaminants from soils. In this study, Sphingobacterium sp. PNL1, isolated from soil, exhibited both tolerance to BDE-209 and plant-growth-promoting (PGP) traits, including production of 1-aminocyclopropane-1-carboxylic acid deaminase, indole-3-acetic acid, ammonia, and siderophore. Notably, the addition of the biological growth factor myo-inositol (MI) enhanced the PGP traits and adhesion capacity of strain PNL1 under BDE-209 stress. Therefore, this study proposed a strategy of combined use of MI and PGPR to assist phytoremediation and highlighted the effect of this combined application on rice seedling growth and soil BDE-209 remediation under BDE-209 stress. The results revealed that the combined application of PNL1 and MI could significantly mitigate the negative effects of BDE-209 on rice, including increased photosynthetic efficiency and reduced oxidative stress, promoting rice seedling growth. Also, the combined use of PNL1 strain and MI enabled the removal rate of soil BDE-209 to reach 64.06%, which was 38.87% higher than that the group without additional addition. Furthermore, the combined treatment could regulate soil enzyme activities (soil urease, sucrase and alkaline phosphatase) and increase the relative abundance of beneficial rhizosphere bacteria (Firmicutes, Myxococcota, etc.), thereby creating a more favorable microenvironment for plant growth. In conclusion, the combined use of PNL1 and MI effectively alleviated BDE-209 stress in rice seedlings and improved the efficiency of soil remediation, providing an eco-friendly method for treating BDE-209 soil contamination.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"269"},"PeriodicalIF":4.2000,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Combined application of Sphingobacterium sp. PNL1 and myo-inositol enhanced the remediation ability of BDE-209-contaminated soil by promoting plant growth, reducing oxidative stress, improving photosynthetic efficiency and degrading BDE-209 in soil.\",\"authors\":\"Jing Ji, Runzhong Wang, Baoying Ma, Tiange Li, Chunfeng Guan\",\"doi\":\"10.1007/s11274-025-04500-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>As a polybrominated diphenyl ether (PBDE) flame retardant, BDE-209 has attracted attention because of its extensive accumulation in soil and toxic effect on plant growth. Plant promoting rhizosphere bacteria (PGPR) assisted phytoremediation is widely recognized for removing contaminants from soils. In this study, Sphingobacterium sp. PNL1, isolated from soil, exhibited both tolerance to BDE-209 and plant-growth-promoting (PGP) traits, including production of 1-aminocyclopropane-1-carboxylic acid deaminase, indole-3-acetic acid, ammonia, and siderophore. Notably, the addition of the biological growth factor myo-inositol (MI) enhanced the PGP traits and adhesion capacity of strain PNL1 under BDE-209 stress. Therefore, this study proposed a strategy of combined use of MI and PGPR to assist phytoremediation and highlighted the effect of this combined application on rice seedling growth and soil BDE-209 remediation under BDE-209 stress. The results revealed that the combined application of PNL1 and MI could significantly mitigate the negative effects of BDE-209 on rice, including increased photosynthetic efficiency and reduced oxidative stress, promoting rice seedling growth. Also, the combined use of PNL1 strain and MI enabled the removal rate of soil BDE-209 to reach 64.06%, which was 38.87% higher than that the group without additional addition. Furthermore, the combined treatment could regulate soil enzyme activities (soil urease, sucrase and alkaline phosphatase) and increase the relative abundance of beneficial rhizosphere bacteria (Firmicutes, Myxococcota, etc.), thereby creating a more favorable microenvironment for plant growth. In conclusion, the combined use of PNL1 and MI effectively alleviated BDE-209 stress in rice seedlings and improved the efficiency of soil remediation, providing an eco-friendly method for treating BDE-209 soil contamination.</p>\",\"PeriodicalId\":23703,\"journal\":{\"name\":\"World journal of microbiology & biotechnology\",\"volume\":\"41 8\",\"pages\":\"269\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"World journal of microbiology & biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s11274-025-04500-w\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of microbiology & biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11274-025-04500-w","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Combined application of Sphingobacterium sp. PNL1 and myo-inositol enhanced the remediation ability of BDE-209-contaminated soil by promoting plant growth, reducing oxidative stress, improving photosynthetic efficiency and degrading BDE-209 in soil.
As a polybrominated diphenyl ether (PBDE) flame retardant, BDE-209 has attracted attention because of its extensive accumulation in soil and toxic effect on plant growth. Plant promoting rhizosphere bacteria (PGPR) assisted phytoremediation is widely recognized for removing contaminants from soils. In this study, Sphingobacterium sp. PNL1, isolated from soil, exhibited both tolerance to BDE-209 and plant-growth-promoting (PGP) traits, including production of 1-aminocyclopropane-1-carboxylic acid deaminase, indole-3-acetic acid, ammonia, and siderophore. Notably, the addition of the biological growth factor myo-inositol (MI) enhanced the PGP traits and adhesion capacity of strain PNL1 under BDE-209 stress. Therefore, this study proposed a strategy of combined use of MI and PGPR to assist phytoremediation and highlighted the effect of this combined application on rice seedling growth and soil BDE-209 remediation under BDE-209 stress. The results revealed that the combined application of PNL1 and MI could significantly mitigate the negative effects of BDE-209 on rice, including increased photosynthetic efficiency and reduced oxidative stress, promoting rice seedling growth. Also, the combined use of PNL1 strain and MI enabled the removal rate of soil BDE-209 to reach 64.06%, which was 38.87% higher than that the group without additional addition. Furthermore, the combined treatment could regulate soil enzyme activities (soil urease, sucrase and alkaline phosphatase) and increase the relative abundance of beneficial rhizosphere bacteria (Firmicutes, Myxococcota, etc.), thereby creating a more favorable microenvironment for plant growth. In conclusion, the combined use of PNL1 and MI effectively alleviated BDE-209 stress in rice seedlings and improved the efficiency of soil remediation, providing an eco-friendly method for treating BDE-209 soil contamination.
期刊介绍：
World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology.
Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions.
Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories:
· Virology
· Simple isolation of microbes from local sources
· Simple descriptions of an environment or reports on a procedure
· Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism
· Data reporting on host response to microbes
· Optimization of a procedure
· Description of the biological effects of not fully identified compounds or undefined extracts of natural origin
· Data on not fully purified enzymes or procedures in which they are applied
All articles published in the Journal are independently refereed.
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