Gabriel Leda de Arruda, Maria Teresa F R Raymundo, Layde Teixeira de Carvalho, Silvio S da Silva, Júlio C Santos
{"title":"用聚氨酯泡沫固定红曲霉细胞从蔗渣半纤维素水解物中生产生物色素。","authors":"Gabriel Leda de Arruda, Maria Teresa F R Raymundo, Layde Teixeira de Carvalho, Silvio S da Silva, Júlio C Santos","doi":"10.1007/s11274-025-04497-2","DOIUrl":null,"url":null,"abstract":"<p><p>The complete use of all fractions of agro-industrial by-products is essential to promote sustainability and consolidated bioprocesses in biorefineries. Among the biomass fractions, hemicellulose is still underutilized and could be used to produce high value bioproducts, as fungal biopigments, emerging biomolecules with potential applications in different industrial sectors. Besides raw material, cultivation strategies such as the use of immobilized cells can be interesting for industrial applications, presenting advantages by favoring downstream steps and reuse of cells in repeated or continuous bioprocesses. In this study, the production of biopigments by Monascus ruber immobilized in polyurethane foam was evaluated as a strategy to valorize a xylose-rich sugarcane bagasse hemicellulosic hydrolysate. An experimental design was carried out and showed the optimum xylose/glucose ratio obtained was similar to the values usually found in sugarcane bagasse hemicellulosic hydrolysates. Then, the kinetic profile of the in situ immobilization of the fungus (simultaneous cell growth and adsorption) in polyurethane indicated an inoculum preparation time of 8 days of growth/immobilization before the process conducted in hydrolysate-based medium. The use of immobilized cells resulted in the production of biopigments with maximum values of 13.1 AU, 11.87 AU, and 11.32 AU of yellow, orange and red biopigments, respectively, in medium based on detoxified hydrolysate. The obtained results can contribute to the valorization of agro-industrial by-products as an alternative source of substrates, as well as to future research to explore the potential of immobilizing filamentous fungi in bioprocesses, such as the reuse of cells and continuous systems for production of biopigments.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"41 8","pages":"289"},"PeriodicalIF":4.2000,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biopigments production from sugarcane bagasse hemicellulosic hydrolysate using cells of Monascus ruber immobilized in polyurethane foam.\",\"authors\":\"Gabriel Leda de Arruda, Maria Teresa F R Raymundo, Layde Teixeira de Carvalho, Silvio S da Silva, Júlio C Santos\",\"doi\":\"10.1007/s11274-025-04497-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The complete use of all fractions of agro-industrial by-products is essential to promote sustainability and consolidated bioprocesses in biorefineries. Among the biomass fractions, hemicellulose is still underutilized and could be used to produce high value bioproducts, as fungal biopigments, emerging biomolecules with potential applications in different industrial sectors. Besides raw material, cultivation strategies such as the use of immobilized cells can be interesting for industrial applications, presenting advantages by favoring downstream steps and reuse of cells in repeated or continuous bioprocesses. In this study, the production of biopigments by Monascus ruber immobilized in polyurethane foam was evaluated as a strategy to valorize a xylose-rich sugarcane bagasse hemicellulosic hydrolysate. An experimental design was carried out and showed the optimum xylose/glucose ratio obtained was similar to the values usually found in sugarcane bagasse hemicellulosic hydrolysates. Then, the kinetic profile of the in situ immobilization of the fungus (simultaneous cell growth and adsorption) in polyurethane indicated an inoculum preparation time of 8 days of growth/immobilization before the process conducted in hydrolysate-based medium. The use of immobilized cells resulted in the production of biopigments with maximum values of 13.1 AU, 11.87 AU, and 11.32 AU of yellow, orange and red biopigments, respectively, in medium based on detoxified hydrolysate. The obtained results can contribute to the valorization of agro-industrial by-products as an alternative source of substrates, as well as to future research to explore the potential of immobilizing filamentous fungi in bioprocesses, such as the reuse of cells and continuous systems for production of biopigments.</p>\",\"PeriodicalId\":23703,\"journal\":{\"name\":\"World journal of microbiology & biotechnology\",\"volume\":\"41 8\",\"pages\":\"289\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"World journal of microbiology & biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s11274-025-04497-2\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of microbiology & biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11274-025-04497-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Biopigments production from sugarcane bagasse hemicellulosic hydrolysate using cells of Monascus ruber immobilized in polyurethane foam.
The complete use of all fractions of agro-industrial by-products is essential to promote sustainability and consolidated bioprocesses in biorefineries. Among the biomass fractions, hemicellulose is still underutilized and could be used to produce high value bioproducts, as fungal biopigments, emerging biomolecules with potential applications in different industrial sectors. Besides raw material, cultivation strategies such as the use of immobilized cells can be interesting for industrial applications, presenting advantages by favoring downstream steps and reuse of cells in repeated or continuous bioprocesses. In this study, the production of biopigments by Monascus ruber immobilized in polyurethane foam was evaluated as a strategy to valorize a xylose-rich sugarcane bagasse hemicellulosic hydrolysate. An experimental design was carried out and showed the optimum xylose/glucose ratio obtained was similar to the values usually found in sugarcane bagasse hemicellulosic hydrolysates. Then, the kinetic profile of the in situ immobilization of the fungus (simultaneous cell growth and adsorption) in polyurethane indicated an inoculum preparation time of 8 days of growth/immobilization before the process conducted in hydrolysate-based medium. The use of immobilized cells resulted in the production of biopigments with maximum values of 13.1 AU, 11.87 AU, and 11.32 AU of yellow, orange and red biopigments, respectively, in medium based on detoxified hydrolysate. The obtained results can contribute to the valorization of agro-industrial by-products as an alternative source of substrates, as well as to future research to explore the potential of immobilizing filamentous fungi in bioprocesses, such as the reuse of cells and continuous systems for production of biopigments.
期刊介绍:
World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology.
Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions.
Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories:
· Virology
· Simple isolation of microbes from local sources
· Simple descriptions of an environment or reports on a procedure
· Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism
· Data reporting on host response to microbes
· Optimization of a procedure
· Description of the biological effects of not fully identified compounds or undefined extracts of natural origin
· Data on not fully purified enzymes or procedures in which they are applied
All articles published in the Journal are independently refereed.