{"title":"西trimide增强氢氧化钙膏：再生牙髓学的生物相容性和生物活性选择。","authors":"Júlia Eick Iglesias, Lucas Siqueira Pinheiro, Gabriela Cardoso Ferreira, Marla Cuppini, Fabrício Mezzomo Collares, Fabiana Soares Grecca","doi":"10.1016/j.joen.2025.07.016","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>This study compared the cytotoxicity, bioactivity, and biocompatibility of calcium hydroxide paste (CHP) with or without 0.2% cetrimide in microspheres (CETm) or in pure form (CET).</p><p><strong>Methods: </strong>CETm or CET were added to CHP and evaluated for cytocompatibility (3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay) and bioactivity (Alizarin Red and alkaline phosphatase), using stem cells from the apical papilla. Biocompatibility was assessed by implanting polyethylene tubes containing the materials into rat subcutaneous tissue. One-way ANOVA followed by Tukey post hoc was used to analyze the results of 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide, alkaline phosphatase, and Alizarin Red assays. Kruskal-Wallis with Dunn's post hoc test was used for the biocompatibility test (P < .05).</p><p><strong>Results: </strong>All pastes exhibited cell viability above 100% and bioactivity in alkaline phosphatase and Alizarin Red assays. No abscesses, eosinophils, neutrophils, or giant cells were observed. After 90 days, all materials induced fibrous condensation, and inflammation decreased from mild to insignificant.</p><p><strong>Conclusion: </strong>Adding CETm or CET to CHP may offer a potential intracanal medication for regenerative endodontic procedures, demonstrating favorable biological properties.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cetrimide-enhanced Calcium Hydroxide Paste: A Biocompatible and Bioactive Option for Regenerative Endodontics.\",\"authors\":\"Júlia Eick Iglesias, Lucas Siqueira Pinheiro, Gabriela Cardoso Ferreira, Marla Cuppini, Fabrício Mezzomo Collares, Fabiana Soares Grecca\",\"doi\":\"10.1016/j.joen.2025.07.016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>This study compared the cytotoxicity, bioactivity, and biocompatibility of calcium hydroxide paste (CHP) with or without 0.2% cetrimide in microspheres (CETm) or in pure form (CET).</p><p><strong>Methods: </strong>CETm or CET were added to CHP and evaluated for cytocompatibility (3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay) and bioactivity (Alizarin Red and alkaline phosphatase), using stem cells from the apical papilla. Biocompatibility was assessed by implanting polyethylene tubes containing the materials into rat subcutaneous tissue. One-way ANOVA followed by Tukey post hoc was used to analyze the results of 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide, alkaline phosphatase, and Alizarin Red assays. Kruskal-Wallis with Dunn's post hoc test was used for the biocompatibility test (P < .05).</p><p><strong>Results: </strong>All pastes exhibited cell viability above 100% and bioactivity in alkaline phosphatase and Alizarin Red assays. No abscesses, eosinophils, neutrophils, or giant cells were observed. After 90 days, all materials induced fibrous condensation, and inflammation decreased from mild to insignificant.</p><p><strong>Conclusion: </strong>Adding CETm or CET to CHP may offer a potential intracanal medication for regenerative endodontic procedures, demonstrating favorable biological properties.</p>\",\"PeriodicalId\":15703,\"journal\":{\"name\":\"Journal of endodontics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of endodontics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.joen.2025.07.016\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of endodontics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.joen.2025.07.016","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Cetrimide-enhanced Calcium Hydroxide Paste: A Biocompatible and Bioactive Option for Regenerative Endodontics.
Introduction: This study compared the cytotoxicity, bioactivity, and biocompatibility of calcium hydroxide paste (CHP) with or without 0.2% cetrimide in microspheres (CETm) or in pure form (CET).
Methods: CETm or CET were added to CHP and evaluated for cytocompatibility (3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay) and bioactivity (Alizarin Red and alkaline phosphatase), using stem cells from the apical papilla. Biocompatibility was assessed by implanting polyethylene tubes containing the materials into rat subcutaneous tissue. One-way ANOVA followed by Tukey post hoc was used to analyze the results of 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide, alkaline phosphatase, and Alizarin Red assays. Kruskal-Wallis with Dunn's post hoc test was used for the biocompatibility test (P < .05).
Results: All pastes exhibited cell viability above 100% and bioactivity in alkaline phosphatase and Alizarin Red assays. No abscesses, eosinophils, neutrophils, or giant cells were observed. After 90 days, all materials induced fibrous condensation, and inflammation decreased from mild to insignificant.
Conclusion: Adding CETm or CET to CHP may offer a potential intracanal medication for regenerative endodontic procedures, demonstrating favorable biological properties.
期刊介绍：
The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.
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