{"title":"MiR-499a-5p通过激活Wnt/β-Catenin信号通路促进子宫平滑肌瘤细胞增殖和迁移","authors":"Jingjing Ni, Yan Ma, Jianping Qiu","doi":"10.2147/IJWH.S533729","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the functional role and underlying mechanism of miR-499a-5p in the progression of uterine leiomyoma (ULM).</p><p><strong>Methods: </strong>Expression levels of miR-499a-5p were analyzed in ULM tissues and cells. Functional assays were conducted to evaluate the effects of miR-499a-5p knockdown or overexpression on cellular proliferation, migration, apoptosis, and cell cycle progression. The involvement of the Wnt/β-catenin signaling pathway was assessed using pathway-specific protein markers and lithium chloride (LiCl) as a chemical activator.</p><p><strong>Results: </strong>miR-499a-5p was significantly upregulated in ULM tissues and cells. Its knockdown inhibited proliferation and migration, induced apoptosis, and caused cell cycle arrest at the G0/G1 phase. Additionally, downregulation of miR-499a-5p suppressed the activation of the Wnt/β-catenin pathway, an effect that was reversed by LiCl treatment.</p><p><strong>Conclusion: </strong>miR-499a-5p facilitates the development of uterine leiomyoma by activating the Wnt/β-catenin signaling pathway. These findings suggest that miR-499a-5p may serve as a promising molecular biomarker and a potential therapeutic target for ULM management.</p>","PeriodicalId":14356,"journal":{"name":"International Journal of Women's Health","volume":"17 ","pages":"2245-2253"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292381/pdf/","citationCount":"0","resultStr":"{\"title\":\"MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway.\",\"authors\":\"Jingjing Ni, Yan Ma, Jianping Qiu\",\"doi\":\"10.2147/IJWH.S533729\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to investigate the functional role and underlying mechanism of miR-499a-5p in the progression of uterine leiomyoma (ULM).</p><p><strong>Methods: </strong>Expression levels of miR-499a-5p were analyzed in ULM tissues and cells. Functional assays were conducted to evaluate the effects of miR-499a-5p knockdown or overexpression on cellular proliferation, migration, apoptosis, and cell cycle progression. The involvement of the Wnt/β-catenin signaling pathway was assessed using pathway-specific protein markers and lithium chloride (LiCl) as a chemical activator.</p><p><strong>Results: </strong>miR-499a-5p was significantly upregulated in ULM tissues and cells. Its knockdown inhibited proliferation and migration, induced apoptosis, and caused cell cycle arrest at the G0/G1 phase. Additionally, downregulation of miR-499a-5p suppressed the activation of the Wnt/β-catenin pathway, an effect that was reversed by LiCl treatment.</p><p><strong>Conclusion: </strong>miR-499a-5p facilitates the development of uterine leiomyoma by activating the Wnt/β-catenin signaling pathway. These findings suggest that miR-499a-5p may serve as a promising molecular biomarker and a potential therapeutic target for ULM management.</p>\",\"PeriodicalId\":14356,\"journal\":{\"name\":\"International Journal of Women's Health\",\"volume\":\"17 \",\"pages\":\"2245-2253\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-07-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292381/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Women's Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/IJWH.S533729\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Women's Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IJWH.S533729","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
MiR-499a-5p Promotes the Proliferation and Migration of Uterine Leiomyoma Cells by Activating the Wnt/β-Catenin Signaling Pathway.
Objective: This study aimed to investigate the functional role and underlying mechanism of miR-499a-5p in the progression of uterine leiomyoma (ULM).
Methods: Expression levels of miR-499a-5p were analyzed in ULM tissues and cells. Functional assays were conducted to evaluate the effects of miR-499a-5p knockdown or overexpression on cellular proliferation, migration, apoptosis, and cell cycle progression. The involvement of the Wnt/β-catenin signaling pathway was assessed using pathway-specific protein markers and lithium chloride (LiCl) as a chemical activator.
Results: miR-499a-5p was significantly upregulated in ULM tissues and cells. Its knockdown inhibited proliferation and migration, induced apoptosis, and caused cell cycle arrest at the G0/G1 phase. Additionally, downregulation of miR-499a-5p suppressed the activation of the Wnt/β-catenin pathway, an effect that was reversed by LiCl treatment.
Conclusion: miR-499a-5p facilitates the development of uterine leiomyoma by activating the Wnt/β-catenin signaling pathway. These findings suggest that miR-499a-5p may serve as a promising molecular biomarker and a potential therapeutic target for ULM management.
期刊介绍：
International Journal of Women''s Health is an international, peer-reviewed, open access, online journal. Publishing original research, reports, editorials, reviews and commentaries on all aspects of women''s healthcare including gynecology, obstetrics, and breast cancer. Subject areas include: Chronic conditions including cancers of various organs specific and not specific to women Migraine, headaches, arthritis, osteoporosis Endocrine and autoimmune syndromes - asthma, multiple sclerosis, lupus, diabetes Sexual and reproductive health including fertility patterns and emerging technologies to address infertility Infectious disease with chronic sequelae including HIV/AIDS, HPV, PID, and other STDs Psychological and psychosocial conditions - depression across the life span, substance abuse, domestic violence Health maintenance among aging females - factors affecting the quality of life including physical, social and mental issues Avenues for health promotion and disease prevention across the life span Male vs female incidence comparisons for conditions that affect both genders.
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