幼年皮肌炎肌肉组织中CD68 +巨噬细胞浸润与病程相关，而与病情严重程度无关。

IF 2.8 3区医学 Q2 RHEUMATOLOGY

Clinical Rheumatology Pub Date : 2025-09-01 Epub Date: 2025-07-28 DOI:10.1007/s10067-025-07601-x

Yun Zhou, Fei Zheng, Yini Wang, Weizhong Gu, Yiping Xu, Liping Teng, Qian Ma, Bin Hu, Meiping Lu, Xuefeng Xu

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The positive area proportions of macrophage infiltrations and T lymphocyte infiltrations in muscle tissue of JDM were linearly correlated (CD68 and CD3 r = 0.592, p < 0.001; CD68 and CD4 r = 0.690, p < 0.001; CD68 and CD8 r = 0.579, p < 0.001; CD163 and CD3 r = 0.649, p < 0.001; CD163 and CD4 r = 0.651, p < 0.001; CD163 and CD8 r = 0.763, p < 0.001). In JDM patients with a disease course < 1 year, the duration of disease is linearly correlated with the degree of CD68 + macrophage infiltrations in muscle tissue, and the longer the disease duration, the more CD68 + macrophage infiltrations in the muscle tissue (r = 0.283, p = 0.028). The positive area proportions of CD68 + macrophages and T lymphocytes (CD3 + , CD4 + , and CD8 +) infiltrating the muscle tissue of anti-PM/Scl75-positive JDM patients were significantly higher than those of anti-PM/Scl75-negative patients. 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引用次数: 0

摘要

目的：探讨青少年皮肌炎（JDM）患者肌肉组织免疫组化特征与临床表型的关系，探讨肌肉组织炎症细胞浸润的影响因素。方法：选取JDM患者19例，对照组5例。对肌肉活检组织进行免疫组化染色。结果：与对照组相比，JDM患者肌肉组织中巨噬细胞和淋巴细胞明显增加。JDM肌肉组织中巨噬细胞浸润阳性面积比例与T淋巴细胞浸润呈线性相关（CD68和CD3 r = 0.592, p）。结论：JDM肌肉组织中CD68 +巨噬细胞浸润与病程相关，与病情严重程度无关。•肌肉组织的炎症细胞浸润与JDM的病程有关，而与严重程度无关。•JDM肌肉组织巨噬细胞浸润与T淋巴细胞浸润呈线性相关。•病程与肌肉组织中CD68 +巨噬细胞浸润呈线性相关。

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CD68 + macrophage infiltrations in muscle tissue of juvenile dermatomyositis associated with the course of the disease, not with the severity.

Objective: To explore the relationship between the immunohistochemical characteristics of muscle tissue and clinical phenotype, and investigate the influencing factors of inflammatory cell infiltrations in muscle tissue in Juvenile dermatomyositis (JDM) patients.

Methods: Nineteen JDM patients and five control patients were included in the present study. Immunohistochemical staining was performed on the muscle biopsies.

Results: The macrophages and lymphocytes significantly increased in muscle tissue of JDM patients compared with the control group. The positive area proportions of macrophage infiltrations and T lymphocyte infiltrations in muscle tissue of JDM were linearly correlated (CD68 and CD3 r = 0.592, p < 0.001; CD68 and CD4 r = 0.690, p < 0.001; CD68 and CD8 r = 0.579, p < 0.001; CD163 and CD3 r = 0.649, p < 0.001; CD163 and CD4 r = 0.651, p < 0.001; CD163 and CD8 r = 0.763, p < 0.001). In JDM patients with a disease course < 1 year, the duration of disease is linearly correlated with the degree of CD68 + macrophage infiltrations in muscle tissue, and the longer the disease duration, the more CD68 + macrophage infiltrations in the muscle tissue (r = 0.283, p = 0.028). The positive area proportions of CD68 + macrophages and T lymphocytes (CD3 + , CD4 + , and CD8 +) infiltrating the muscle tissue of anti-PM/Scl75-positive JDM patients were significantly higher than those of anti-PM/Scl75-negative patients. The degree of T lymphocyte (CD3 + , CD4 + , and CD8 +) infiltrations in the muscle tissue of anti-NXP2-positive JDM patients was significantly lower than those of anti-NXP2-negative patients.

Conclusion: The CD68 + macrophages infiltrations of muscle tissues in JDM associated with the course of JDM, not related to disease severity. Key Points • The inflammatory cell infiltrations of muscle tissues associated with the course of JDM, not severity. • Macrophage infiltrations in muscle tissue of JDM are linearly correlated with T lymphocyte infiltrations. • The duration of disease is linearly correlated with CD68 + macrophage infiltrations in muscle tissue.

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来源期刊

Clinical Rheumatology 医学-风湿病学

CiteScore

6.90

自引率

2.90%

发文量

441

审稿时长

3 months

期刊介绍： Clinical Rheumatology is an international English-language journal devoted to publishing original clinical investigation and research in the general field of rheumatology with accent on clinical aspects at postgraduate level. The journal succeeds Acta Rheumatologica Belgica, originally founded in 1945 as the official journal of the Belgian Rheumatology Society. Clinical Rheumatology aims to cover all modern trends in clinical and experimental research as well as the management and evaluation of diagnostic and treatment procedures connected with the inflammatory, immunologic, metabolic, genetic and degenerative soft and hard connective tissue diseases.