一个28.5 kDa的a类扩展光谱β-内酰胺酶的高场碳检测固体核磁共振骨架分配。

IF 0.6 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-07-27 DOI:10.1007/s12104-025-10242-5

Christopher G Williams, Songlin Wang, Alexander F Thome, Owen A Warmuth, Varun Sakhrani, Leighton Coates, Chad M Rienstra, Leonard J Mueller

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引用次数: 0

摘要

报道了28.5 kDa蛋白Toho-1 β-内酰胺酶（a类扩展谱β-内酰胺酶）的13C和15N主链化学位移定位。通过超高场核磁共振波谱（1.1 GHz）的灵敏度和分辨率增益、改进的探针技术和优化的脉冲序列，可以实现非常高水平的分配完整性（97%的骨干）。分配的化学位移与我们之前的溶液态NMR分配非常一致，表明二级结构在固态中是守恒的。这些作业为今后研究侧链化学位移和催化机理提供了基础。

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Backbone assignment of a 28.5 kDa class A extended spectrum β-lactamase by high-field, carbon-detected solid-state NMR.

¹³C and ¹⁵N backbone chemical shift assignments are reported for the 28.5 kDa protein Toho-1 β-lactamase, a Class A extended spectrum β-lactamase. A very high level of assignment completeness (97% of the backbone) is enabled by the combined sensitivity and resolution gains of ultrahigh-field NMR spectroscopy (1.1 GHz), improved probe technology, and optimized pulse sequences. The assigned chemical shifts agree well with our previous solution-state NMR assignments, indicating that the secondary structure is conserved in the solid state. These assignments provide a foundation for future investigations of side-chain chemical shifts and catalytic mechanism.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.