利用溶液态核磁共振光谱分析斑马鱼P18ink4c的1H， 13C， 15N主链化学位移。

IF 0.6 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-07-28 DOI:10.1007/s12104-025-10245-2

Aakriti Sethi, Pierre de Cordovez, Biswaranjan Mohanty, Vanessa K Morris, Christoph Göbl

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引用次数: 0

摘要

INK4蛋白家族通过抑制周期蛋白依赖性激酶4和6来限制不受控制的细胞周期进程。该家族由小的，单体的，主要是α -螺旋蛋白组成，在所有脊椎动物物种中都是保守的。我们最近发现，人类INK4蛋白p16在单一半胱氨酸残基氧化后转化为淀粉样蛋白结构。在这里，我们研究了斑马鱼同源P18蛋白。170个残基蛋白含有两种半胱氨酸，它们在氧化时同样可以介导转化为淀粉样蛋白。我们提出了在溶液中还原的P18蛋白的几乎完整的骨架分配。这些化学位移数据为研究氧化诱导的结构变化和蛋白质相互作用提供了基础。

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¹H, ¹³C, ¹⁵N backbone chemical shift assignment of P18ink4c from Danio rerio (zebrafish) using solution-state NMR spectroscopy.

The INK4 family of proteins restricts uncontrolled cell cycle progression by inhibiting cyclin-dependent kinases 4 and 6. The family consists of small, monomeric and mainly alpha-helical proteins that are conserved across all vertebrate species. We recently discovered that the human INK4 protein p16 converts into amyloid structures upon oxidation of the single cysteine residue present. Here we investigate the Danio rerio (zebrafish) orthologue P18 protein. The 170-residue protein contains two cysteines which may similarly mediate transition into amyloids upon oxidation. We present the near complete backbone assignment of the reduced P18 protein in solution. These chemical shift data provide the foundation for studying oxidation-induced structural changes and protein interactions.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.