小鼠耳蜗单细胞RNA测序分析在年龄相关性和噪声性听力损失小鼠中发现了一种新的促炎性CD74+CD14+巨噬细胞亚群

IF 2.5 2区医学 Q1 AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY

Hearing Research Pub Date : 2025-07-24 DOI:10.1016/j.heares.2025.109376

Min-Yu Wu , An-Hai Chen , Lin-Qiu Li , Ying Yi, Qin Xiong, Kai-Tian Chen, Zhi-Mou Cai, Wen-Bin Lei, Guan-Xia Xiong, Shu-Bin Fang

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引用次数: 0

摘要

年龄相关性听力损失（ARHL）和噪声性听力损失（NIHL）都与免疫反应的激活有关。然而，ARHL和NIHL耳蜗内的免疫微环境仍然知之甚少。因此，我们使用单细胞RNA测序分析ARHL和NIHL的免疫细胞。我们观察到，在ARHL和NIHL中，CD74+CD14+巨噬细胞（Mφ）增加，这一亚群与炎症途径、免疫细胞趋化和其他参与炎症反应的过程有关。ARHL和NIHL与螺旋神经节神经元变性有关。我们发现在两种情况下，CD74+CD14+ Mφ与螺旋神经节神经元之间的细胞通讯增强，其中CD74+CD14+ Mφ是ARHL中与螺旋神经节神经元相互作用最大的免疫细胞。在本研究中，我们强调了Mφ在ARHL和NIHL中共同和独特的特征，为这两种类型的听力损失提供了新的潜在治疗靶点。

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Single-cell RNA sequencing analysis of mouse cochlea identifies a novel proinflammatory CD74+CD14+ macrophage subset in mice with age-related and noise-induced hearing loss

Age-related hearing loss (ARHL) and noise-induced hearing loss (NIHL) are both associated with the activation of immune responses. However, the immune microenvironment within the cochlea in ARHL and NIHL remains poorly understood. Therefore, we profiled the immune cells in ARHL and NIHL using single-cell RNA sequencing analyses. We observed an increase in CD74⁺CD14⁺ macrophages (Mφ) in ARHL and NIHL, with this subset being associated with inflammatory pathways, immune cell chemotaxis and other processes that involved in inflammatory responses. ARHL and NIHL are linked to the degeneration of spiral ganglion neurons. We demonstrated enhanced cellular communication between CD74⁺CD14⁺ Mφ and spiral ganglion neurons in both conditions, with CD74⁺CD14⁺ Mφ being the most interactive immune cells with spiral ganglion neurons in ARHL. In this study, we highlight the common and distinct characteristics of Mφ in ARHL and NIHL, providing new potential therapeutic targets for these two types of hearing loss.

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来源期刊

Hearing Research 医学-耳鼻喉科学

CiteScore

5.30

自引率

14.30%

发文量

163

审稿时长

75 days

期刊介绍： The aim of the journal is to provide a forum for papers concerned with basic peripheral and central auditory mechanisms. Emphasis is on experimental and clinical studies, but theoretical and methodological papers will also be considered. The journal publishes original research papers, review and mini- review articles, rapid communications, method/protocol and perspective articles. Papers submitted should deal with auditory anatomy, physiology, psychophysics, imaging, modeling and behavioural studies in animals and humans, as well as hearing aids and cochlear implants. Papers dealing with the vestibular system are also considered for publication. Papers on comparative aspects of hearing and on effects of drugs and environmental contaminants on hearing function will also be considered. Clinical papers will be accepted when they contribute to the understanding of normal and pathological hearing functions.