用于生物传感应用的CRISPR/Cas12a工程分子开关

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-07-22 DOI:10.1039/d5cc03652f

Menghui Wang , Yin Wang , Jialin Wen , Bo Wang , Gaoyang Lu , Xiao-Bing Zhang , Mei Chen , Guoliang Ke

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引用次数: 0

摘要

CRISPR/Cas12a系统凭借其高特异性、优异的可编程性和高效的信号扩增能力，已成为分子诊断领域的重要工具。为了充分发挥Cas12a的应用潜力，近年来人们开发了许多CRISPR/Cas12a的分子开关，将Cas12a的活性从非活性状态转变为响应外界刺激的活性状态，从而显著提高了CRISPR/Cas12a的生物传感性能。Cas12a。灵敏度，选择性和准确性。本文综述了近年来基于cas12的分子开关的开发和应用进展，重点介绍了它们的条件激活机制和生物传感应用。讨论了智能生物传感器的发展面临的挑战和未来的发展前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Engineering molecular switches of CRISPR/Cas12a for biosensing applications

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Engineering molecular switches of CRISPR/Cas12a for biosensing applications

Benefiting from high specificity, excellent programmability, and efficient signal amplification capability, the CRISPR/Cas12a system has emerged as a crucial tool in the field of molecular diagnostics. To fully realize the application potential of Cas12a, many molecular switches of CRISPR/Cas12a have been recently developed to convert the activity of Cas12a from an inactive state to an active state in response to external stimuli, which significantly improves the biosensing performance in terms of the sensitivity, selectivity, and accuracy. This review summarizes recent progress in the development and application of Cas12a-based molecular switches, with a focus on their conditional activation mechanisms and biosensing applications. Additionally, challenges and future perspectives for the development of intelligent biosensors are discussed.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.