原生肺和肺植入物血液性扩散后原发性肺结核小鼠模型的建立。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-07-25 DOI:10.1016/j.xpro.2025.103984

Shivraj M Yabaji, Suruchi Lata, Igor Gavrish, Ming Lo, Aoife K O'Connell, Hans P Gertje, Colleen E Thurman, Nicholas A Crossland, Lester Kobzik, Igor Kramnik

{"title":"原生肺和肺植入物血液性扩散后原发性肺结核小鼠模型的建立。","authors":"Shivraj M Yabaji, Suruchi Lata, Igor Gavrish, Ming Lo, Aoife K O'Connell, Hans P Gertje, Colleen E Thurman, Nicholas A Crossland, Lester Kobzik, Igor Kramnik","doi":"10.1016/j.xpro.2025.103984","DOIUrl":null,"url":null,"abstract":"Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung and spleen implantation, preparation of Mtb for infection, and hock infection of mice. We then detail procedures for the perfusion of the lung and collection of organs, tissue processing, and histopathologic interpretation. For complete details on the use and execution of this protocol, please refer to Yabaji et al.1,2.","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 3","pages":"103984"},"PeriodicalIF":1.3000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants.\",\"authors\":\"Shivraj M Yabaji, Suruchi Lata, Igor Gavrish, Ming Lo, Aoife K O'Connell, Hans P Gertje, Colleen E Thurman, Nicholas A Crossland, Lester Kobzik, Igor Kramnik\",\"doi\":\"10.1016/j.xpro.2025.103984\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung and spleen implantation, preparation of Mtb for infection, and hock infection of mice. We then detail procedures for the perfusion of the lung and collection of organs, tissue processing, and histopathologic interpretation. For complete details on the use and execution of this protocol, please refer to Yabaji et al.1,2.\",\"PeriodicalId\":34214,\"journal\":{\"name\":\"STAR Protocols\",\"volume\":\"6 3\",\"pages\":\"103984\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"STAR Protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xpro.2025.103984\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2025.103984","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

在这里，我们提出了一种方案，用于研究小鼠模型的机制由毒力结核分枝杆菌（Mtb）引起的原发性肺结核（PTB），通过皮下感染和肺组织植入。我们描述了肺胶原蛋白滴注、肺脾植入、结核分枝杆菌感染制备和小鼠跗关节感染的步骤。然后我们详细介绍了肺灌注和器官收集、组织处理和组织病理学解释的程序。有关本协议使用和执行的完整细节，请参阅Yabaji等人1,2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants.

Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung and spleen implantation, preparation of Mtb for infection, and hock infection of mice. We then detail procedures for the perfusion of the lung and collection of organs, tissue processing, and histopathologic interpretation. For complete details on the use and execution of this protocol, please refer to Yabaji et al.¹^,².

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊