节肢动物P450百科：一个用于研究P450的综合网络资源。

IF 3.7 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-24 DOI:10.1016/j.ibmb.2025.104377

Jason Charamis , Wannes Dermauw , Thomas Van Leeuwen , John Vontas , René Feyereisen

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引用次数: 0

摘要

我们介绍了节肢动物P450 Enchiridion (arthropodp450.eu)，这是一个基于DokuWiki架构的网站，旨在提供来自最多样化和数量最多的动物节肢动物的CYP基因和P450酶的结构和功能资源。相互链接的页面涵盖了P450酶的一些基本共同特征，从命名规则到酶的机制和蛋白质结构。具体到节肢动物，查找表允许将目前1308个CYP家族快速分配到9个各自的CYP氏族，以及从完整cypome到氏族的p450分布。人工标注了目前75个物种的cypome，提供了正确的CYP名称以及近3000个全长的命名昆虫转录本。节肢动物P450 Enchiridion提供BLAST功能，定位超过11,000个精确命名的P450蛋白序列。通过体外异源表达、RNAi表型、转基因体内表达或无突变体表型来评估P450功能的案例超过1000例。这些数据如预期的那样显示了对昆虫和螨虫害虫物种的关注，但也突出了我们对较少研究的节肢动物群体中p450的序列和功能的了解仍然存在空白。DokuWiki的体系结构支持定期更新和扩展，欢迎作者对此做出贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The arthropod P450 Enchiridion: An integrated web resource for research on P450s

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The arthropod P450 Enchiridion: An integrated web resource for research on P450s

We present the Arthropod P450 Enchiridion (arthropodp450.eu), a website based on the DokuWiki architecture and designed to provide resources on structures and functions of CYP genes and P450 enzymes from the most diverse and numerous animals, the arthropods. Interlinked pages cover some essential common features of P450 enzymes from rules of nomenclature to enzyme mechanism and protein structure. Specific to arthropods, lookup tables allow the rapid assignment of currently 1308 CYP families to their nine respective CYP clans, as well as the distribution of P450s from full CYPomes into clans. Manually annotated CYPomes from currently 75 species are presented with their correct CYP names as well as nearly 3000 full-length, named insect transcripts. The Arthropod P450 Enchiridion offers a BLAST function addressing over 11,000 accurately named P450 protein sequences. P450 functions as assessed by in vitro heterologous expression, RNAi phenotype, transgenic in vivo expression, or the phenotype of null mutants are presented for over 1000 cases. The data show as expected a focus on insect and mite pest species, but also highlights the remaining gaps in our knowledge of sequence and function of P450s in less studied groups of arthropods. The architecture of the DokuWiki enables regular updates and expansions to which authors are welcome to contribute.

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来源期刊

Insect Biochemistry and Molecular Biology 生物-昆虫学

CiteScore

7.40

自引率

5.30%

发文量

105

审稿时长

40 days

期刊介绍： This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.