犬成纤维细胞直接重编程诱导运动神经元的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-07-24 DOI:10.1016/j.xpro.2025.103975

Ryan S Anderson, Amelia C Corona, Masatoshi Suzuki, Susannah J Sample

{"title":"犬成纤维细胞直接重编程诱导运动神经元的方案。","authors":"Ryan S Anderson, Amelia C Corona, Masatoshi Suzuki, Susannah J Sample","doi":"10.1016/j.xpro.2025.103975","DOIUrl":null,"url":null,"abstract":"Direct reprogramming of canine fibroblasts to induced motor neurons in vitro opens the door to molecular characterization, functional analysis, and therapeutic screening of canine age-related neurodegenerative diseases. Here, we present a protocol for generating canine induced motor neurons directly from primary dermal fibroblasts. We provide a step-by-step guide for isolating and maintaining primary dermal fibroblasts from dogs, obtaining a stock of reprogrammable cells, and performing the direct reprogramming process. We then detail procedures for validating the motor neuron identity.","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 3","pages":"103975"},"PeriodicalIF":1.3000,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protocol for direct reprogramming of canine fibroblasts to induced motor neurons.\",\"authors\":\"Ryan S Anderson, Amelia C Corona, Masatoshi Suzuki, Susannah J Sample\",\"doi\":\"10.1016/j.xpro.2025.103975\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Direct reprogramming of canine fibroblasts to induced motor neurons in vitro opens the door to molecular characterization, functional analysis, and therapeutic screening of canine age-related neurodegenerative diseases. Here, we present a protocol for generating canine induced motor neurons directly from primary dermal fibroblasts. We provide a step-by-step guide for isolating and maintaining primary dermal fibroblasts from dogs, obtaining a stock of reprogrammable cells, and performing the direct reprogramming process. We then detail procedures for validating the motor neuron identity.\",\"PeriodicalId\":34214,\"journal\":{\"name\":\"STAR Protocols\",\"volume\":\"6 3\",\"pages\":\"103975\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"STAR Protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xpro.2025.103975\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2025.103975","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

犬成纤维细胞在体外直接重编程诱导运动神经元，为犬年龄相关神经退行性疾病的分子表征、功能分析和治疗筛选打开了大门。在这里，我们提出了一种直接从原代真皮成纤维细胞产生犬诱导运动神经元的方案。我们提供一步一步的指导，分离和维持原代真皮成纤维细胞从狗，获得一个库存的可重编程细胞，并执行直接重编程过程。然后，我们详细说明了验证运动神经元身份的程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Protocol for direct reprogramming of canine fibroblasts to induced motor neurons.

Direct reprogramming of canine fibroblasts to induced motor neurons in vitro opens the door to molecular characterization, functional analysis, and therapeutic screening of canine age-related neurodegenerative diseases. Here, we present a protocol for generating canine induced motor neurons directly from primary dermal fibroblasts. We provide a step-by-step guide for isolating and maintaining primary dermal fibroblasts from dogs, obtaining a stock of reprogrammable cells, and performing the direct reprogramming process. We then detail procedures for validating the motor neuron identity.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊