IL-34在胰腺中表达并调节局部炎症。

IF 5.9 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Chloé M Delépine , Emilie Courty , Laurence Preisser , Raffaella Soleti , Laetitia Basset , Audrey Le Guernic , Laure Rolland , Marine Monnier , Gianni Pasquetti , Simon Blanchard , Pascale Pignon , Nathalie Merillon , Julie Kerr-Conte , Charline Miot , Dominique Couez , Aline Schmidt-Tanguy , Elise Dalmas , Pascale Jeannin , Jean-Sébastien Annicotte , Yves Delneste , Celine Beauvillain
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引用次数: 0

摘要

白细胞介素-34是CD115的配体,控制朗格汉斯细胞和小胶质细胞的稳态,并作为调节细胞因子。在这里,我们描述了IL-34在代谢稳态中的一个意想不到的作用。我们发现IL-34在人β细胞和小鼠朗格汉斯胰岛中组成性表达。小鼠中il -34的β细胞特异性遗传失能(IL-34ΔPdx)与衰老过程中朗格汉斯胰岛炎症增加相关的糖耐量受损。当IL-34ΔPdx小鼠接受高脂肪饮食时,这种表型会加剧,这表明IL-34在控制局部炎症中的作用。因此,在促炎细胞因子的存在下,朗格汉斯离体小鼠胰岛表现出胰岛素合成减少,在IL-34的存在下恢复。最后,我们观察到来自2型糖尿病供体的朗格汉斯胰岛Il-34 mRNA水平升高。总之,这些结果确定IL-34是胰腺炎症的主要调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IL-34 is expressed in the pancreas and regulates local inflammation
Interleukin-34, a ligand of CD115, controls Langerhans cells and microglia homeostasis and acts as regulatory cytokine. Here, we describe an unexpected role for IL-34 in metabolic homeostasis. We show that IL-34 is constitutively expressed by human β-cell and mouse islets of Langerhans. β-cell specific genetic invalidation of Il34 in mice (IL-34ΔPdx) impaired glucose tolerance associated with increased inflammation in islets of Langerhans during aging. This phenotype was exacerbated when IL-34ΔPdx mice were subjected to a high-fat diet, suggesting a role for IL-34 in controlling local inflammation. Accordingly, in the presence of pro-inflammatory cytokines, ex vivo murine islets of Langerhans exhibited a decreased insulin synthesis, which was restored in the presence of IL-34. Finally, we observed that the levels of Il-34 mRNA were elevated in islets of Langerhans from type 2 diabetic donors. Collectively, these results identify IL-34 as a major regulator of pancreas inflammation.
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来源期刊
Translational Research
Translational Research 医学-医学:内科
CiteScore
15.70
自引率
0.00%
发文量
195
审稿时长
14 days
期刊介绍: Translational Research (formerly The Journal of Laboratory and Clinical Medicine) delivers original investigations in the broad fields of laboratory, clinical, and public health research. Published monthly since 1915, it keeps readers up-to-date on significant biomedical research from all subspecialties of medicine.
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