Felice Emanuela Espèrance van Veen, Zhaleh Esmi Serkani, Sophie Berendsen, Robert Kraaij, Lonneke Bode, John Philip Hays, Jeroen Ronald Scheepe, Bertil Freddo Maarten Blok
{"title":"使用一次性导管与可重复使用导管进行间歇导尿的女性尿液微生物组：比较试验的探索性亚研究。","authors":"Felice Emanuela Espèrance van Veen, Zhaleh Esmi Serkani, Sophie Berendsen, Robert Kraaij, Lonneke Bode, John Philip Hays, Jeroen Ronald Scheepe, Bertil Freddo Maarten Blok","doi":"10.1002/nau.70119","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>To characterize the urinary microbiome in women performing clean intermittent self-catheterization (CISC) and explore microbial changes associated with transitioning from single-use to reusable catheters.</p><p><strong>Methods: </strong>This microbiome study of the COMPaRE trial included female CISC patients with urinary retention randomized to either single-use or reusable catheters. Self-catheterized urine samples were collected at baseline and Week 6 for standard culture and 16S rRNA microbiome analysis.</p><p><strong>Results: </strong>A total of 28 patients (12 reusable, 16 single-use) were included, with a median age of 48 years (IQR 32-60); 67.9% had neurogenic lower urinary tract dysfunction. Escherichia-Shigella (36.3%) and Lactobacillus (22.8%) were the most prevalent genera. PERMANOVA identified significant effects of age (p = 0.003), menopausal status (p < 0.001), and catheterization cause (p = 0.003) on microbiome composition. Transitioning to reusable catheters was associated with significant reductions in Escherichia-Shigella (p < 0.001) and Aerococcus (p < 0.001), and increases in Veillonella (p < 0.001) and Finegoldia (p < 0.001). No significant changes were observed in urine culture results (p = 0.250), alpha diversity as measured by the Shannon index (p = 0.862), or beta diversity as assessed by Bray-Curtis distance (p = 0.096).</p><p><strong>Conclusions: </strong>Escherichia-Shigella and Lactobacillus were the most abundant genera in the urinary microbiome of women performing CISC. Age, menopausal status, and catheterization cause significantly influenced microbiome composition. Although specific microbial shifts were observed following transition to reusable catheters, no significant changes in overall microbial diversity were detected. Larger, well-powered studies are needed to validate these results and assess their potential clinical implications.</p><p><strong>Trial registration: </strong>Clinical Trial RegistrationNL-OMON54700/NL8296.</p>","PeriodicalId":19200,"journal":{"name":"Neurourology and Urodynamics","volume":" ","pages":"1474-1483"},"PeriodicalIF":1.9000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12319515/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Urinary Microbiome in Women Using Single-Use Versus Reusable Catheters for Intermittent Catheterization: An Exploratory Substudy of the COMPaRE Trial.\",\"authors\":\"Felice Emanuela Espèrance van Veen, Zhaleh Esmi Serkani, Sophie Berendsen, Robert Kraaij, Lonneke Bode, John Philip Hays, Jeroen Ronald Scheepe, Bertil Freddo Maarten Blok\",\"doi\":\"10.1002/nau.70119\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>To characterize the urinary microbiome in women performing clean intermittent self-catheterization (CISC) and explore microbial changes associated with transitioning from single-use to reusable catheters.</p><p><strong>Methods: </strong>This microbiome study of the COMPaRE trial included female CISC patients with urinary retention randomized to either single-use or reusable catheters. Self-catheterized urine samples were collected at baseline and Week 6 for standard culture and 16S rRNA microbiome analysis.</p><p><strong>Results: </strong>A total of 28 patients (12 reusable, 16 single-use) were included, with a median age of 48 years (IQR 32-60); 67.9% had neurogenic lower urinary tract dysfunction. Escherichia-Shigella (36.3%) and Lactobacillus (22.8%) were the most prevalent genera. PERMANOVA identified significant effects of age (p = 0.003), menopausal status (p < 0.001), and catheterization cause (p = 0.003) on microbiome composition. Transitioning to reusable catheters was associated with significant reductions in Escherichia-Shigella (p < 0.001) and Aerococcus (p < 0.001), and increases in Veillonella (p < 0.001) and Finegoldia (p < 0.001). No significant changes were observed in urine culture results (p = 0.250), alpha diversity as measured by the Shannon index (p = 0.862), or beta diversity as assessed by Bray-Curtis distance (p = 0.096).</p><p><strong>Conclusions: </strong>Escherichia-Shigella and Lactobacillus were the most abundant genera in the urinary microbiome of women performing CISC. Age, menopausal status, and catheterization cause significantly influenced microbiome composition. Although specific microbial shifts were observed following transition to reusable catheters, no significant changes in overall microbial diversity were detected. Larger, well-powered studies are needed to validate these results and assess their potential clinical implications.</p><p><strong>Trial registration: </strong>Clinical Trial RegistrationNL-OMON54700/NL8296.</p>\",\"PeriodicalId\":19200,\"journal\":{\"name\":\"Neurourology and Urodynamics\",\"volume\":\" \",\"pages\":\"1474-1483\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12319515/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neurourology and Urodynamics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/nau.70119\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"UROLOGY & NEPHROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurourology and Urodynamics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/nau.70119","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/24 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
The Urinary Microbiome in Women Using Single-Use Versus Reusable Catheters for Intermittent Catheterization: An Exploratory Substudy of the COMPaRE Trial.
Aims: To characterize the urinary microbiome in women performing clean intermittent self-catheterization (CISC) and explore microbial changes associated with transitioning from single-use to reusable catheters.
Methods: This microbiome study of the COMPaRE trial included female CISC patients with urinary retention randomized to either single-use or reusable catheters. Self-catheterized urine samples were collected at baseline and Week 6 for standard culture and 16S rRNA microbiome analysis.
Results: A total of 28 patients (12 reusable, 16 single-use) were included, with a median age of 48 years (IQR 32-60); 67.9% had neurogenic lower urinary tract dysfunction. Escherichia-Shigella (36.3%) and Lactobacillus (22.8%) were the most prevalent genera. PERMANOVA identified significant effects of age (p = 0.003), menopausal status (p < 0.001), and catheterization cause (p = 0.003) on microbiome composition. Transitioning to reusable catheters was associated with significant reductions in Escherichia-Shigella (p < 0.001) and Aerococcus (p < 0.001), and increases in Veillonella (p < 0.001) and Finegoldia (p < 0.001). No significant changes were observed in urine culture results (p = 0.250), alpha diversity as measured by the Shannon index (p = 0.862), or beta diversity as assessed by Bray-Curtis distance (p = 0.096).
Conclusions: Escherichia-Shigella and Lactobacillus were the most abundant genera in the urinary microbiome of women performing CISC. Age, menopausal status, and catheterization cause significantly influenced microbiome composition. Although specific microbial shifts were observed following transition to reusable catheters, no significant changes in overall microbial diversity were detected. Larger, well-powered studies are needed to validate these results and assess their potential clinical implications.
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Neurourology and Urodynamics welcomes original scientific contributions from all parts of the world on topics related to urinary tract function, urinary and fecal continence and pelvic floor function.
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