Marwa A. Ali, Olfat G. Shaker, Eman M. Ezzat, Mohamed Saad Zaghloul Ahmed, Shimaa Mohammed Elasmer, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Zaidan, Raghda Yahia Abu El-Ela, Marwa N. AbdelHafez, Eman Fares, Ahmad A. Al Abdulqader, Aeshah A. Awaji, Mariam K. Alamoudi, Omayma O. Abdelaleem
{"title":"血清Circ-DLGAP4和miR-9作为胰腺癌的潜在生物标志物","authors":"Marwa A. Ali, Olfat G. Shaker, Eman M. Ezzat, Mohamed Saad Zaghloul Ahmed, Shimaa Mohammed Elasmer, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Zaidan, Raghda Yahia Abu El-Ela, Marwa N. AbdelHafez, Eman Fares, Ahmad A. Al Abdulqader, Aeshah A. Awaji, Mariam K. Alamoudi, Omayma O. Abdelaleem","doi":"10.1002/jcla.70076","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Pancreatic cancer remains one of the malignancies characterized by the most insidious onset and dire prognosis. Preliminary diagnostic biomarkers are essential for timely identification and improved prognosis. CircularRNA-DLGAP4 (Circ-DLGAP4) and microRNA-9 (miR-9) are intriguing, easily accessible genetic indicators for numerous diseases.</p>\n </section>\n \n <section>\n \n <h3> Aim</h3>\n \n <p>To quantify serum expression levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients and juxtapose these values with those of control persons to assess their potential as diagnostic biomarkers. It also aims to correlate their expression levels with the clinicopathological features of the disease.</p>\n </section>\n \n <section>\n \n <h3> Method</h3>\n \n <p>Quantitative analysis of serum samples collected from 40 pancreatic cancer patients and 40 control subjects with RT-PCR.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>There were statistically significant elevations in blood levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients compared to healthy persons, with median (range) values of 8.2 (0.39–17.32), <i>p</i>-value < 0.001, and 9.5 (4.06–14.7), respectively. The blood level of Circ-DLGAP4 exhibited a significant positive correlation with the serum concentration of miR-9 (<i>r</i> = 0.89, <i>p</i> = 0.001).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Circ-DLGAP4 and miR-9 serve as diagnostic biomarkers for pancreatic cancer.</p>\n </section>\n </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 16","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70076","citationCount":"0","resultStr":"{\"title\":\"Serum Circ-DLGAP4 and miR-9 as Potential Biomarkers in Pancreatic Cancer\",\"authors\":\"Marwa A. Ali, Olfat G. Shaker, Eman M. Ezzat, Mohamed Saad Zaghloul Ahmed, Shimaa Mohammed Elasmer, Marwa Mamdouh Ahmed Abdelhafeez, Mohamed Zaidan, Raghda Yahia Abu El-Ela, Marwa N. AbdelHafez, Eman Fares, Ahmad A. Al Abdulqader, Aeshah A. Awaji, Mariam K. Alamoudi, Omayma O. Abdelaleem\",\"doi\":\"10.1002/jcla.70076\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Pancreatic cancer remains one of the malignancies characterized by the most insidious onset and dire prognosis. Preliminary diagnostic biomarkers are essential for timely identification and improved prognosis. CircularRNA-DLGAP4 (Circ-DLGAP4) and microRNA-9 (miR-9) are intriguing, easily accessible genetic indicators for numerous diseases.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>To quantify serum expression levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients and juxtapose these values with those of control persons to assess their potential as diagnostic biomarkers. It also aims to correlate their expression levels with the clinicopathological features of the disease.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Method</h3>\\n \\n <p>Quantitative analysis of serum samples collected from 40 pancreatic cancer patients and 40 control subjects with RT-PCR.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>There were statistically significant elevations in blood levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients compared to healthy persons, with median (range) values of 8.2 (0.39–17.32), <i>p</i>-value < 0.001, and 9.5 (4.06–14.7), respectively. The blood level of Circ-DLGAP4 exhibited a significant positive correlation with the serum concentration of miR-9 (<i>r</i> = 0.89, <i>p</i> = 0.001).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Circ-DLGAP4 and miR-9 serve as diagnostic biomarkers for pancreatic cancer.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15509,\"journal\":{\"name\":\"Journal of Clinical Laboratory Analysis\",\"volume\":\"39 16\",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70076\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Clinical Laboratory Analysis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jcla.70076\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Laboratory Analysis","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jcla.70076","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Serum Circ-DLGAP4 and miR-9 as Potential Biomarkers in Pancreatic Cancer
Background
Pancreatic cancer remains one of the malignancies characterized by the most insidious onset and dire prognosis. Preliminary diagnostic biomarkers are essential for timely identification and improved prognosis. CircularRNA-DLGAP4 (Circ-DLGAP4) and microRNA-9 (miR-9) are intriguing, easily accessible genetic indicators for numerous diseases.
Aim
To quantify serum expression levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients and juxtapose these values with those of control persons to assess their potential as diagnostic biomarkers. It also aims to correlate their expression levels with the clinicopathological features of the disease.
Method
Quantitative analysis of serum samples collected from 40 pancreatic cancer patients and 40 control subjects with RT-PCR.
Results
There were statistically significant elevations in blood levels of Circ-DLGAP4 and miR-9 in pancreatic cancer patients compared to healthy persons, with median (range) values of 8.2 (0.39–17.32), p-value < 0.001, and 9.5 (4.06–14.7), respectively. The blood level of Circ-DLGAP4 exhibited a significant positive correlation with the serum concentration of miR-9 (r = 0.89, p = 0.001).
Conclusions
Circ-DLGAP4 and miR-9 serve as diagnostic biomarkers for pancreatic cancer.
期刊介绍：
Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
