{"title":"意大利移民中寨卡病毒和登革热病毒感染的血清流行率","authors":"Federica Frasca, Francesco Eugenio Romani, Elio Gentilini Cacciola, Francesca Colavita, Enrico Palermo, Luca Maddaloni, Luigi Rosa, Alessandra D'Auria, Valentina Baccolini, Giuseppe Migliara, Giulia Matusali, Giancarlo Ceccarelli, Guido Antonelli, Fabrizio Maggi, Gabriella d'Ettorre, Carolina Scagnolari","doi":"10.3389/fcimb.2025.1617029","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Estimating the burden of Zika virus (ZIKV) and dengue virus (DENV) infections in migrants is important to promote their health status and recommend appropriate interventions. We aimed to estimate the seroprevalence of ZIKV and DENV in migrants from high endemic countries attended at a referral center in Rome (Italy), arriving via the Mediterranean from North and sub-Saharan Africa and South-East Asia.</p><p><strong>Methods: </strong>Sixty-four serum samples from migrants were tested for anti-ZIKV and anti-DENV immunoglobulin (Ig) G and IgM by ELISA. Serum samples with detectable Ig were analyzed by indirect immunofluorescence assay (IFA). For confirmatory testing and given the cross-reactivity of antibodies between orthoflaviviruses, all positive IFA sera were tested by virus neutralization test. ZIKV and DENV RNA were assessed by RT Real-Time PCR.</p><p><strong>Results: </strong>All serum samples were negative for anti-ZIKV IgG, while 12.50% (n=8/64) were positive for anti-ZIKV IgM by ELISA. IFA showed that only 1 of 8 serum samples (12.50%) was positive for anti-ZIKV IgM, but ZIKV RNA was undetectable. The seroprevalence of anti-DENV IgG by ELISA was 59.37% (n=38/64), mostly confirmed by IFA (97.36%, n=37/38). Furthermore, anti-DENV IgM were detected in 9 serum samples (n=9/64, 14.06%) by ELISA, previously tested negative for anti-DENV IgG. Of these, 2 sera were confirmed by IFA, but DENV RNA was not detectable. Anti-DENV neutralizing antibodies (nAbs) were detected in 27% of anti-DENV IgG sera (n=10/37) tested by IFA. Multiple linear regression analysis showed that sub-Saharan African origin was an independent factor for the development of anti-DENV nAbs (p=0.009), while age and gender had no effect. Sera negatives for anti-DENV nAbs but with detectable anti-DENV IgG tested by IFA had nAbs to another orthoflavivirus (n=25/27, 92.59%) such as West Nile virus (WNV) (n=17/25, 68%), Yellow fever virus (YFV) (n=7/25, 28%) and Usutu virus (USUV) (n=1/25, 4%).</p><p><strong>Discussion: </strong>A high prevalence of anti-orthoflavivirus IgG, especially against DENV, was found in the migrant population studied, but no infections were detected. With the recent outbreaks of autochthonous DENV infections in Italy, the risk of secondary DENV infection - and severe disease - could be high. Robust serological surveillance, vaccination and prevention strategies for this vulnerable group are needed.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1617029"},"PeriodicalIF":4.8000,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287079/pdf/","citationCount":"0","resultStr":"{\"title\":\"Seroprevalence of Zika virus and dengue virus infections in migrants in Italy.\",\"authors\":\"Federica Frasca, Francesco Eugenio Romani, Elio Gentilini Cacciola, Francesca Colavita, Enrico Palermo, Luca Maddaloni, Luigi Rosa, Alessandra D'Auria, Valentina Baccolini, Giuseppe Migliara, Giulia Matusali, Giancarlo Ceccarelli, Guido Antonelli, Fabrizio Maggi, Gabriella d'Ettorre, Carolina Scagnolari\",\"doi\":\"10.3389/fcimb.2025.1617029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Estimating the burden of Zika virus (ZIKV) and dengue virus (DENV) infections in migrants is important to promote their health status and recommend appropriate interventions. We aimed to estimate the seroprevalence of ZIKV and DENV in migrants from high endemic countries attended at a referral center in Rome (Italy), arriving via the Mediterranean from North and sub-Saharan Africa and South-East Asia.</p><p><strong>Methods: </strong>Sixty-four serum samples from migrants were tested for anti-ZIKV and anti-DENV immunoglobulin (Ig) G and IgM by ELISA. Serum samples with detectable Ig were analyzed by indirect immunofluorescence assay (IFA). For confirmatory testing and given the cross-reactivity of antibodies between orthoflaviviruses, all positive IFA sera were tested by virus neutralization test. ZIKV and DENV RNA were assessed by RT Real-Time PCR.</p><p><strong>Results: </strong>All serum samples were negative for anti-ZIKV IgG, while 12.50% (n=8/64) were positive for anti-ZIKV IgM by ELISA. IFA showed that only 1 of 8 serum samples (12.50%) was positive for anti-ZIKV IgM, but ZIKV RNA was undetectable. The seroprevalence of anti-DENV IgG by ELISA was 59.37% (n=38/64), mostly confirmed by IFA (97.36%, n=37/38). Furthermore, anti-DENV IgM were detected in 9 serum samples (n=9/64, 14.06%) by ELISA, previously tested negative for anti-DENV IgG. Of these, 2 sera were confirmed by IFA, but DENV RNA was not detectable. Anti-DENV neutralizing antibodies (nAbs) were detected in 27% of anti-DENV IgG sera (n=10/37) tested by IFA. Multiple linear regression analysis showed that sub-Saharan African origin was an independent factor for the development of anti-DENV nAbs (p=0.009), while age and gender had no effect. Sera negatives for anti-DENV nAbs but with detectable anti-DENV IgG tested by IFA had nAbs to another orthoflavivirus (n=25/27, 92.59%) such as West Nile virus (WNV) (n=17/25, 68%), Yellow fever virus (YFV) (n=7/25, 28%) and Usutu virus (USUV) (n=1/25, 4%).</p><p><strong>Discussion: </strong>A high prevalence of anti-orthoflavivirus IgG, especially against DENV, was found in the migrant population studied, but no infections were detected. With the recent outbreaks of autochthonous DENV infections in Italy, the risk of secondary DENV infection - and severe disease - could be high. Robust serological surveillance, vaccination and prevention strategies for this vulnerable group are needed.</p>\",\"PeriodicalId\":12458,\"journal\":{\"name\":\"Frontiers in Cellular and Infection Microbiology\",\"volume\":\"15 \",\"pages\":\"1617029\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2025-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287079/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Cellular and Infection Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/fcimb.2025.1617029\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2025.1617029","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Seroprevalence of Zika virus and dengue virus infections in migrants in Italy.
Introduction: Estimating the burden of Zika virus (ZIKV) and dengue virus (DENV) infections in migrants is important to promote their health status and recommend appropriate interventions. We aimed to estimate the seroprevalence of ZIKV and DENV in migrants from high endemic countries attended at a referral center in Rome (Italy), arriving via the Mediterranean from North and sub-Saharan Africa and South-East Asia.
Methods: Sixty-four serum samples from migrants were tested for anti-ZIKV and anti-DENV immunoglobulin (Ig) G and IgM by ELISA. Serum samples with detectable Ig were analyzed by indirect immunofluorescence assay (IFA). For confirmatory testing and given the cross-reactivity of antibodies between orthoflaviviruses, all positive IFA sera were tested by virus neutralization test. ZIKV and DENV RNA were assessed by RT Real-Time PCR.
Results: All serum samples were negative for anti-ZIKV IgG, while 12.50% (n=8/64) were positive for anti-ZIKV IgM by ELISA. IFA showed that only 1 of 8 serum samples (12.50%) was positive for anti-ZIKV IgM, but ZIKV RNA was undetectable. The seroprevalence of anti-DENV IgG by ELISA was 59.37% (n=38/64), mostly confirmed by IFA (97.36%, n=37/38). Furthermore, anti-DENV IgM were detected in 9 serum samples (n=9/64, 14.06%) by ELISA, previously tested negative for anti-DENV IgG. Of these, 2 sera were confirmed by IFA, but DENV RNA was not detectable. Anti-DENV neutralizing antibodies (nAbs) were detected in 27% of anti-DENV IgG sera (n=10/37) tested by IFA. Multiple linear regression analysis showed that sub-Saharan African origin was an independent factor for the development of anti-DENV nAbs (p=0.009), while age and gender had no effect. Sera negatives for anti-DENV nAbs but with detectable anti-DENV IgG tested by IFA had nAbs to another orthoflavivirus (n=25/27, 92.59%) such as West Nile virus (WNV) (n=17/25, 68%), Yellow fever virus (YFV) (n=7/25, 28%) and Usutu virus (USUV) (n=1/25, 4%).
Discussion: A high prevalence of anti-orthoflavivirus IgG, especially against DENV, was found in the migrant population studied, but no infections were detected. With the recent outbreaks of autochthonous DENV infections in Italy, the risk of secondary DENV infection - and severe disease - could be high. Robust serological surveillance, vaccination and prevention strategies for this vulnerable group are needed.
期刊介绍：
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
