{"title":"壳聚糖/海藻酸盐复合材料中双负载ZnO和Se纳米粒子的合成、细胞毒性、抗氧化活性和抗宫颈癌潜力","authors":"Zahra Amiri, Shahrzad Shahbazi, Somayeh Reiisi, Razieh Heidari, Mansour Ghorbanpour","doi":"10.1007/s13205-025-04436-w","DOIUrl":null,"url":null,"abstract":"<p><p>Nanomaterials, encompassing nanoparticles and biopolymers, are gaining recognition as potential therapeutic agents. This study explored the antioxidant and anticancer effects of zinc oxide and selenium co-loaded chitosan/alginate bio-composites against the HeLa human cervical cancer cell line. Zinc oxide and selenium nanoparticles were synthesized using a green synthesis approach and subsequently integrated into a chitosan/alginate bio-composite, designated as hydro ZnO/Se NPs. The physicochemical characteristics were analyzed through Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), zeta potential measurements, and electron microscopy (SEM/TEM). The antioxidant efficacy of the hydro ZnO/Se NPs was assessed via a DPPH scavenging assay. Human dermal fibroblast (HDF) and HeLa cells were subjected to various concentrations of hydro ZnO/Se NPs, with evaluations of cell viability, migration, and colony formation conducted through MTT, wound healing, and clonogenic assays, respectively. Additionally, quantitative polymerase chain reaction (qPCR) was utilized to examine the expression of apoptosis-related genes, specifically BCL2, caspase3, caspase8, and caspase9. Characterization techniques confirmed the successful synthesis of pure, crystalline, and spherical hydro ZnO/Se NPs. Antioxidant assays revealed substantial DPPH free radical-scavenging activity of the hydro ZnO/Se NPs. In vitro analyses demonstrated that hydro ZnO/Se NPs significantly inhibited cell proliferation, reduced migration, and diminished colony formation, likely due to the upregulation of CASP3, CASP8, and CASP9 gene expression. These findings highlight the therapeutic potential of hydro ZnO/Se NPs as promising anticancer agents for cervical cancer.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04436-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"264"},"PeriodicalIF":2.9000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283538/pdf/","citationCount":"0","resultStr":"{\"title\":\"Dual-loaded ZnO and Se nanoparticles in chitosan/alginate bio-composite: synthesis, cytotoxicity, antioxidant activity, and anti-cervical cancer potential.\",\"authors\":\"Zahra Amiri, Shahrzad Shahbazi, Somayeh Reiisi, Razieh Heidari, Mansour Ghorbanpour\",\"doi\":\"10.1007/s13205-025-04436-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Nanomaterials, encompassing nanoparticles and biopolymers, are gaining recognition as potential therapeutic agents. This study explored the antioxidant and anticancer effects of zinc oxide and selenium co-loaded chitosan/alginate bio-composites against the HeLa human cervical cancer cell line. Zinc oxide and selenium nanoparticles were synthesized using a green synthesis approach and subsequently integrated into a chitosan/alginate bio-composite, designated as hydro ZnO/Se NPs. The physicochemical characteristics were analyzed through Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), zeta potential measurements, and electron microscopy (SEM/TEM). The antioxidant efficacy of the hydro ZnO/Se NPs was assessed via a DPPH scavenging assay. Human dermal fibroblast (HDF) and HeLa cells were subjected to various concentrations of hydro ZnO/Se NPs, with evaluations of cell viability, migration, and colony formation conducted through MTT, wound healing, and clonogenic assays, respectively. Additionally, quantitative polymerase chain reaction (qPCR) was utilized to examine the expression of apoptosis-related genes, specifically BCL2, caspase3, caspase8, and caspase9. Characterization techniques confirmed the successful synthesis of pure, crystalline, and spherical hydro ZnO/Se NPs. Antioxidant assays revealed substantial DPPH free radical-scavenging activity of the hydro ZnO/Se NPs. In vitro analyses demonstrated that hydro ZnO/Se NPs significantly inhibited cell proliferation, reduced migration, and diminished colony formation, likely due to the upregulation of CASP3, CASP8, and CASP9 gene expression. 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Dual-loaded ZnO and Se nanoparticles in chitosan/alginate bio-composite: synthesis, cytotoxicity, antioxidant activity, and anti-cervical cancer potential.
Nanomaterials, encompassing nanoparticles and biopolymers, are gaining recognition as potential therapeutic agents. This study explored the antioxidant and anticancer effects of zinc oxide and selenium co-loaded chitosan/alginate bio-composites against the HeLa human cervical cancer cell line. Zinc oxide and selenium nanoparticles were synthesized using a green synthesis approach and subsequently integrated into a chitosan/alginate bio-composite, designated as hydro ZnO/Se NPs. The physicochemical characteristics were analyzed through Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), zeta potential measurements, and electron microscopy (SEM/TEM). The antioxidant efficacy of the hydro ZnO/Se NPs was assessed via a DPPH scavenging assay. Human dermal fibroblast (HDF) and HeLa cells were subjected to various concentrations of hydro ZnO/Se NPs, with evaluations of cell viability, migration, and colony formation conducted through MTT, wound healing, and clonogenic assays, respectively. Additionally, quantitative polymerase chain reaction (qPCR) was utilized to examine the expression of apoptosis-related genes, specifically BCL2, caspase3, caspase8, and caspase9. Characterization techniques confirmed the successful synthesis of pure, crystalline, and spherical hydro ZnO/Se NPs. Antioxidant assays revealed substantial DPPH free radical-scavenging activity of the hydro ZnO/Se NPs. In vitro analyses demonstrated that hydro ZnO/Se NPs significantly inhibited cell proliferation, reduced migration, and diminished colony formation, likely due to the upregulation of CASP3, CASP8, and CASP9 gene expression. These findings highlight the therapeutic potential of hydro ZnO/Se NPs as promising anticancer agents for cervical cancer.
Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04436-w.
3 BiotechAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍：
3 Biotech publishes the results of the latest research related to the study and application of biotechnology to:
- Medicine and Biomedical Sciences
- Agriculture
- The Environment
The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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