Maria Juliana Sismeiro Dias Morabito, Gabriel Pereira Nunes, Edilson Ervolino, Maria José Hitomi Nagata, Francisco Nunes de Souza Neto, Emerson Rodrigues de Camargo, Alberto Carlos Botazzo Delbem
{"title":"纳米银增强三偏磷酸酯纳米复合材料聚酰胺-6骨再生支架的体内研究","authors":"Maria Juliana Sismeiro Dias Morabito, Gabriel Pereira Nunes, Edilson Ervolino, Maria José Hitomi Nagata, Francisco Nunes de Souza Neto, Emerson Rodrigues de Camargo, Alberto Carlos Botazzo Delbem","doi":"10.1002/jbm.b.35623","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The aim of this study was to evaluate the influence of nanocomposite materials, specifically sodium trimetaphosphate (TMP) nanoparticles decorated with 2.5% silver nanoparticles (AgNP), incorporated into polyamide-6 (Pa6) polymeric matrices on bone repair in surgically created critical-size defects in rat calvaria. Eighteen male Wistar rats were divided into three groups based on the treatment received: control group (C)—blood clot; Pa6-AgNP; and Pa6-TMP-AgNP. A 5 mm diameter defect was created in the calvaria of each animal. In Group C, the defect was filled with blood clots only, while the other groups were filled with scaffolds containing Pa6-AgNP and Pa6-TMP-AgNP. After 30 days post-surgery, the animals were euthanized, and microtomographic, histomorphometric, and immunohistochemical analyses were performed. The area of newly formed bone was calculated as a percentage of the total area of the original defect, and immunostaining for TGFβ-1, BMP-2/4, and OCN was assessed. Intergroup analysis revealed that the Pa6-TMP-AgNP group exhibited a greater volume of newly formed bone, while the Pa6-AgNP group showed a significantly increased bone formation compared to the control group (<i>p</i> < 0.05). Immunohistochemistry indicated that the Pa6-TMP-AgNP and Pa6-AgNP groups showed high and moderate immunostaining for TGFβ-1 and OCN, respectively. Additionally, the Pa6-TMP-AgNP group had a higher number of BMP-2/4 positive cells compared to the other groups (<i>p</i> < 0.001). Based on these results, it can be concluded that Pa6-TMP-AgNP provides a biological response conducive to bone neoformation, exhibiting osteoinductive and osteoconductive properties when used to fill critical-size defects in rat calvaria.</p>\n </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 8","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vivo Assessment of Trimetaphosphate Nanocomposite-Based Polyamide-6 Scaffolds Enhanced With Silver Nanoparticles for Bone Regeneration\",\"authors\":\"Maria Juliana Sismeiro Dias Morabito, Gabriel Pereira Nunes, Edilson Ervolino, Maria José Hitomi Nagata, Francisco Nunes de Souza Neto, Emerson Rodrigues de Camargo, Alberto Carlos Botazzo Delbem\",\"doi\":\"10.1002/jbm.b.35623\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>The aim of this study was to evaluate the influence of nanocomposite materials, specifically sodium trimetaphosphate (TMP) nanoparticles decorated with 2.5% silver nanoparticles (AgNP), incorporated into polyamide-6 (Pa6) polymeric matrices on bone repair in surgically created critical-size defects in rat calvaria. Eighteen male Wistar rats were divided into three groups based on the treatment received: control group (C)—blood clot; Pa6-AgNP; and Pa6-TMP-AgNP. A 5 mm diameter defect was created in the calvaria of each animal. In Group C, the defect was filled with blood clots only, while the other groups were filled with scaffolds containing Pa6-AgNP and Pa6-TMP-AgNP. After 30 days post-surgery, the animals were euthanized, and microtomographic, histomorphometric, and immunohistochemical analyses were performed. The area of newly formed bone was calculated as a percentage of the total area of the original defect, and immunostaining for TGFβ-1, BMP-2/4, and OCN was assessed. Intergroup analysis revealed that the Pa6-TMP-AgNP group exhibited a greater volume of newly formed bone, while the Pa6-AgNP group showed a significantly increased bone formation compared to the control group (<i>p</i> < 0.05). Immunohistochemistry indicated that the Pa6-TMP-AgNP and Pa6-AgNP groups showed high and moderate immunostaining for TGFβ-1 and OCN, respectively. Additionally, the Pa6-TMP-AgNP group had a higher number of BMP-2/4 positive cells compared to the other groups (<i>p</i> < 0.001). Based on these results, it can be concluded that Pa6-TMP-AgNP provides a biological response conducive to bone neoformation, exhibiting osteoinductive and osteoconductive properties when used to fill critical-size defects in rat calvaria.</p>\\n </div>\",\"PeriodicalId\":15269,\"journal\":{\"name\":\"Journal of biomedical materials research. 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In Vivo Assessment of Trimetaphosphate Nanocomposite-Based Polyamide-6 Scaffolds Enhanced With Silver Nanoparticles for Bone Regeneration
The aim of this study was to evaluate the influence of nanocomposite materials, specifically sodium trimetaphosphate (TMP) nanoparticles decorated with 2.5% silver nanoparticles (AgNP), incorporated into polyamide-6 (Pa6) polymeric matrices on bone repair in surgically created critical-size defects in rat calvaria. Eighteen male Wistar rats were divided into three groups based on the treatment received: control group (C)—blood clot; Pa6-AgNP; and Pa6-TMP-AgNP. A 5 mm diameter defect was created in the calvaria of each animal. In Group C, the defect was filled with blood clots only, while the other groups were filled with scaffolds containing Pa6-AgNP and Pa6-TMP-AgNP. After 30 days post-surgery, the animals were euthanized, and microtomographic, histomorphometric, and immunohistochemical analyses were performed. The area of newly formed bone was calculated as a percentage of the total area of the original defect, and immunostaining for TGFβ-1, BMP-2/4, and OCN was assessed. Intergroup analysis revealed that the Pa6-TMP-AgNP group exhibited a greater volume of newly formed bone, while the Pa6-AgNP group showed a significantly increased bone formation compared to the control group (p < 0.05). Immunohistochemistry indicated that the Pa6-TMP-AgNP and Pa6-AgNP groups showed high and moderate immunostaining for TGFβ-1 and OCN, respectively. Additionally, the Pa6-TMP-AgNP group had a higher number of BMP-2/4 positive cells compared to the other groups (p < 0.001). Based on these results, it can be concluded that Pa6-TMP-AgNP provides a biological response conducive to bone neoformation, exhibiting osteoinductive and osteoconductive properties when used to fill critical-size defects in rat calvaria.
期刊介绍：
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats:
• original research reports
• short research and development reports
• scientific reviews
• current concepts articles
• special reports
• editorials
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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