血清miR-98-5p在肺炎支原体感染合并消化系统损伤患儿中的临床意义

IF 2.3 4区医学 Q3 IMMUNOLOGY

Immunobiology Pub Date : 2025-07-01 DOI:10.1016/j.imbio.2025.153101

Ying Li , Wei Jia , Jiayang Huang , Jian Liu

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引用次数: 0

摘要

研究表明，血清microRNA检测在提高肺炎支原体肺炎（MPP）的诊断准确性方面具有重要的潜力。目的探讨miR-98-5p在肺炎支原体（MP）感染并发腹泻患儿中的表达及临床价值。方法选取122例诊断为MPP的儿童作为研究对象，80例健康儿童作为对照。采用实时荧光定量PCR （RT-qPCR）检测miR-98-5p的表达。采用受试者工作特征（ROC）曲线确定miR-98-5p的诊断价值。采用logistic回归模型分析MPP合并腹泻的危险因素。建立miR-98-5p过表达和敲低的THP-1细胞模型，探讨miR-98-5p在MP感染中的潜在机制。结果血清miR-98-5p在MPP患儿中表达降低，且与logMP-DNA水平呈负相关。伴有腹泻的MPP患儿miR-98-5p水平低于单纯MPP患儿。miR-98-5p对MPP合并腹泻具有良好的诊断潜力，是MPP感染儿童腹泻的危险因素。TargetScan数据库预测显示，miR-98-5p的潜在靶基因，包括Synaptotagmin 1（SYT1）和tribles pseudokinase 1(TRIB1)，都与MP感染诱导的消化系统损伤有关。结论血清miR-98-5p在MPP合并腹泻患儿中表达降低，可能成为诊断MPP的一种生物标志物。

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Clinical significance of serum miR-98-5p in children with Mycoplasma pneumoniae infection complicated with digestive system damage

Background

Studies have shown that serum microRNA detection holds significant potential in improving the diagnostic accuracy of mycoplasma pneumoniae pneumonia (MPP).

Aims

To investigate the expression and clinical value of miR-98-5p in children with Mycoplasma pneumoniae (MP) infection complicated with diarrhea.

Methods

A total of 122 children diagnosed with MPP were selected as study subjects, and 80 healthy children were selected as controls. Real-time quantitative PCR (RT-qPCR) was used to detect the expression of miR-98-5p. Receiver operating characteristic (ROC) curve was used to determine the diagnostic value of miR-98-5p. The risk factors for MPP with diarrhea were analyzed by means of a logistic regression model. THP-1 cell models with miR-98-5p overexpression and knockdown were established to investigate the potential mechanisms of miR-98-5p in MP infection.

Results

Serum miR-98-5p expression is decreased among children having MPP, and it has a negative correlation with the logMP-DNA level. The miR-98-5p level in children with MPP accompanied by diarrhea is lower compared to that in children with MPP alone. miR-98-5p demonstrates promising diagnostic potential for MPP complicated with diarrhea, and serves as a risk factor for diarrhea in MP-infected children. TargetScan database prediction showed that potential target genes of miR-98-5p, including Synaptotagmin 1 (SYT1) and Tribbles pseudokinase 1(TRIB1), are both associated with digestive system injury induced by MP infection.

Conclusions

The expression of serum miR-98-5p is decreased in children with MPP complicated by diarrhea, and it may become a diagnostic biomarker.

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.