{"title":"低密度中性粒细胞：了解口腔鳞状细胞癌肿瘤微环境的关键组成部分。","authors":"Thayalan Dineshkumar, Nirmala Anandan, Rajkumar Krishnan, Indumathi Nandhakumar","doi":"10.4103/jomfp.jomfp_351_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Tumour microenvironment is one of the evading research studies in the progression of cancers. Circulating neutrophil's contribution to tumor growth and metastatic progression has been a matter of research interest. The phenotypic subset of low-density neutrophils (LDNs) has been seen as closely associated with disease severity in various cancers.</p><p><strong>Aim and objectives: </strong>This study aims to investigate the LDN and normal-density neutrophil (NDN) populations in oral cancer.</p><p><strong>Materials and methods: </strong>This is prospective research in which the CD16+ population was magnetically sorted and leukocytes were separated in a density gradient. Using a flow cytometer, the percentages of the neutrophil subpopulation were ascertained. It was established that there are two distinct populations of human neutrophils: LDNs and NDNs.</p><p><strong>Results: </strong>Patients with oral squamous cell carcinoma (OSCC) showed a higher percentage of LDN/NDN ratio and a correspondingly lower percentage of LDN/NDN ratio when compared to the control group. In addition, patients with OSCC had higher neutrophil-to-lymphocyte ratios (NLRs).</p><p><strong>Conclusion: </strong>The current study showed a significant correlation between the LDN/NDN ratio in OSCC, which could be used as a benchmark for novel treatments. Additionally, the acquired LDNs/NDNs and NLR levels in cancer patients demonstrate their importance in OSCC patient prognosis and diagnosis.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 2","pages":"172-178"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283039/pdf/","citationCount":"0","resultStr":"{\"title\":\"Low-density neutrophils: A critical component in understanding the tumor microenvironment of oral squamous cell carcinoma.\",\"authors\":\"Thayalan Dineshkumar, Nirmala Anandan, Rajkumar Krishnan, Indumathi Nandhakumar\",\"doi\":\"10.4103/jomfp.jomfp_351_24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Tumour microenvironment is one of the evading research studies in the progression of cancers. Circulating neutrophil's contribution to tumor growth and metastatic progression has been a matter of research interest. The phenotypic subset of low-density neutrophils (LDNs) has been seen as closely associated with disease severity in various cancers.</p><p><strong>Aim and objectives: </strong>This study aims to investigate the LDN and normal-density neutrophil (NDN) populations in oral cancer.</p><p><strong>Materials and methods: </strong>This is prospective research in which the CD16+ population was magnetically sorted and leukocytes were separated in a density gradient. Using a flow cytometer, the percentages of the neutrophil subpopulation were ascertained. It was established that there are two distinct populations of human neutrophils: LDNs and NDNs.</p><p><strong>Results: </strong>Patients with oral squamous cell carcinoma (OSCC) showed a higher percentage of LDN/NDN ratio and a correspondingly lower percentage of LDN/NDN ratio when compared to the control group. In addition, patients with OSCC had higher neutrophil-to-lymphocyte ratios (NLRs).</p><p><strong>Conclusion: </strong>The current study showed a significant correlation between the LDN/NDN ratio in OSCC, which could be used as a benchmark for novel treatments. Additionally, the acquired LDNs/NDNs and NLR levels in cancer patients demonstrate their importance in OSCC patient prognosis and diagnosis.</p>\",\"PeriodicalId\":38846,\"journal\":{\"name\":\"Journal of Oral and Maxillofacial Pathology\",\"volume\":\"29 2\",\"pages\":\"172-178\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283039/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral and Maxillofacial Pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jomfp.jomfp_351_24\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral and Maxillofacial Pathology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jomfp.jomfp_351_24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/30 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Low-density neutrophils: A critical component in understanding the tumor microenvironment of oral squamous cell carcinoma.
Background: Tumour microenvironment is one of the evading research studies in the progression of cancers. Circulating neutrophil's contribution to tumor growth and metastatic progression has been a matter of research interest. The phenotypic subset of low-density neutrophils (LDNs) has been seen as closely associated with disease severity in various cancers.
Aim and objectives: This study aims to investigate the LDN and normal-density neutrophil (NDN) populations in oral cancer.
Materials and methods: This is prospective research in which the CD16+ population was magnetically sorted and leukocytes were separated in a density gradient. Using a flow cytometer, the percentages of the neutrophil subpopulation were ascertained. It was established that there are two distinct populations of human neutrophils: LDNs and NDNs.
Results: Patients with oral squamous cell carcinoma (OSCC) showed a higher percentage of LDN/NDN ratio and a correspondingly lower percentage of LDN/NDN ratio when compared to the control group. In addition, patients with OSCC had higher neutrophil-to-lymphocyte ratios (NLRs).
Conclusion: The current study showed a significant correlation between the LDN/NDN ratio in OSCC, which could be used as a benchmark for novel treatments. Additionally, the acquired LDNs/NDNs and NLR levels in cancer patients demonstrate their importance in OSCC patient prognosis and diagnosis.
期刊介绍：
The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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