Dongxiao Liu, Jialin Fan, Yi Ye, Yu Liu, Sichao Ren, Wenjing Lei, Xingrui Zhang, Ancheng He, Junqiang Xing, Qinfu Sun, Li Lin, Youping Wang, Jian Wu
{"title":"转录因子BnaWRKY75通过促进油菜水杨酸的生物合成来增强油菜对坏死性菌核病的抗性。","authors":"Dongxiao Liu, Jialin Fan, Yi Ye, Yu Liu, Sichao Ren, Wenjing Lei, Xingrui Zhang, Ancheng He, Junqiang Xing, Qinfu Sun, Li Lin, Youping Wang, Jian Wu","doi":"10.1093/jxb/eraf332","DOIUrl":null,"url":null,"abstract":"<p><p>Sclerotinia sclerotiorum, a devastating necrotrophic fungus causing stem rot in oilseed rape, results in significant global yield losses. Deciphering plant-S. sclerotiorum interactions is crucial for disease control. Here, we report that the transcription factor BnaWRKY75 positively regulates resistance to this pathogen. BnaWRKY75 was the most highly induced WRKY genes upon S. sclerotiorum infection. Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum, whereas BnaWRKY75 knockout plants exhibited reduced resistance. RNA sequencing and DNA affinity purification sequencing showed that the isochorismate synthase gene BnaICS1, a key gene required for salicylic acid (SA) biosynthesis, is a potential target of BnaA10.WRKY75. Yeast one-hybrid, dual-luciferase, and electrophoresis mobility shift assays indicated that BnaA10.WRKY75 directly binds to the BnaCnn.ICS1 promoter to activate its transcription, thereby promoting SA biosynthesis and activating SA-dependent defenses. Genetic analysis in Arabidopsis confirmed ICS1 functions downstream of BnaA10.WRKY75. SA treatment significantly enhanced resistance to S. sclerotiorum in oilseed rape, further highlighting the importance of SA in defense against this pathogen. Finally, we observed differences in transcriptional regulation among BnaICS1 homologous genes in allotetraploid oilseed rape. Our findings suggest that the BnaWRKY75-BnaICS1 module regulates SA-dependent resistance to S. sclerotiorum, expanding our understanding of plant immune responses to necrotrophic pathogens.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The transcription factor BnaWRKY75 enhances resistance to the necrotrophic pathogen Sclerotinia sclerotiorum by promoting salicylic acid biosynthesis in oilseed rape.\",\"authors\":\"Dongxiao Liu, Jialin Fan, Yi Ye, Yu Liu, Sichao Ren, Wenjing Lei, Xingrui Zhang, Ancheng He, Junqiang Xing, Qinfu Sun, Li Lin, Youping Wang, Jian Wu\",\"doi\":\"10.1093/jxb/eraf332\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sclerotinia sclerotiorum, a devastating necrotrophic fungus causing stem rot in oilseed rape, results in significant global yield losses. Deciphering plant-S. sclerotiorum interactions is crucial for disease control. Here, we report that the transcription factor BnaWRKY75 positively regulates resistance to this pathogen. BnaWRKY75 was the most highly induced WRKY genes upon S. sclerotiorum infection. Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum, whereas BnaWRKY75 knockout plants exhibited reduced resistance. RNA sequencing and DNA affinity purification sequencing showed that the isochorismate synthase gene BnaICS1, a key gene required for salicylic acid (SA) biosynthesis, is a potential target of BnaA10.WRKY75. Yeast one-hybrid, dual-luciferase, and electrophoresis mobility shift assays indicated that BnaA10.WRKY75 directly binds to the BnaCnn.ICS1 promoter to activate its transcription, thereby promoting SA biosynthesis and activating SA-dependent defenses. Genetic analysis in Arabidopsis confirmed ICS1 functions downstream of BnaA10.WRKY75. SA treatment significantly enhanced resistance to S. sclerotiorum in oilseed rape, further highlighting the importance of SA in defense against this pathogen. Finally, we observed differences in transcriptional regulation among BnaICS1 homologous genes in allotetraploid oilseed rape. Our findings suggest that the BnaWRKY75-BnaICS1 module regulates SA-dependent resistance to S. sclerotiorum, expanding our understanding of plant immune responses to necrotrophic pathogens.</p>\",\"PeriodicalId\":15820,\"journal\":{\"name\":\"Journal of Experimental Botany\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2025-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jxb/eraf332\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jxb/eraf332","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
The transcription factor BnaWRKY75 enhances resistance to the necrotrophic pathogen Sclerotinia sclerotiorum by promoting salicylic acid biosynthesis in oilseed rape.
Sclerotinia sclerotiorum, a devastating necrotrophic fungus causing stem rot in oilseed rape, results in significant global yield losses. Deciphering plant-S. sclerotiorum interactions is crucial for disease control. Here, we report that the transcription factor BnaWRKY75 positively regulates resistance to this pathogen. BnaWRKY75 was the most highly induced WRKY genes upon S. sclerotiorum infection. Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum, whereas BnaWRKY75 knockout plants exhibited reduced resistance. RNA sequencing and DNA affinity purification sequencing showed that the isochorismate synthase gene BnaICS1, a key gene required for salicylic acid (SA) biosynthesis, is a potential target of BnaA10.WRKY75. Yeast one-hybrid, dual-luciferase, and electrophoresis mobility shift assays indicated that BnaA10.WRKY75 directly binds to the BnaCnn.ICS1 promoter to activate its transcription, thereby promoting SA biosynthesis and activating SA-dependent defenses. Genetic analysis in Arabidopsis confirmed ICS1 functions downstream of BnaA10.WRKY75. SA treatment significantly enhanced resistance to S. sclerotiorum in oilseed rape, further highlighting the importance of SA in defense against this pathogen. Finally, we observed differences in transcriptional regulation among BnaICS1 homologous genes in allotetraploid oilseed rape. Our findings suggest that the BnaWRKY75-BnaICS1 module regulates SA-dependent resistance to S. sclerotiorum, expanding our understanding of plant immune responses to necrotrophic pathogens.
期刊介绍：
The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology.
Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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