细胞病变效应和免疫酶微中和试验检测风疹病毒特异性抗体

IF 1.6 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2025-07-21 DOI:10.1016/j.jviromet.2025.115228

Iris Medits-Weiss, Heidemarie Holzmann, Lukas Weseslindtner, Karin Stiasny

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引用次数: 0

摘要

背景妊娠早期的流行性感冒可引起严重的先天性畸形，但可以通过接种疫苗来预防。对风疹的免疫力通常通过检测风疹病毒特异性抗体来评估。中和试验（nt）具有最高的诊断意义，因为它们测量抗体阻断病毒感染的有效性。目的风疹病毒引起细胞病变效应（CPE）的能力有限。因此，大多数NT格式是基于免疫酶技术检测感染细胞中的病毒蛋白。由于Vero细胞已被描述为对风疹病毒感染具有更明显的CPE易感，我们的目标是用这些细胞开发NT。方法采用已知IgG ELISA浓度（IU/ml）的42份人多克隆样品，比较不同传染性检测方法（免疫酶法和CPE法）的风疹NTs。结果风疹病毒特异性ELISA IgG单位与两种不同NT检测的中和效价以及两种NT格式检测的中和效价呈正相关。结论CPE法检测nt滴度是一种可靠的风疹血清学检测方法。

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Detecting rubella virus-specific antibodies by cytopathic effect and immunoenzymatic microneutralization tests

Background

Rubella in early pregnancy can cause severe congenital malformations, but can be prevented by vaccination. Immunity to rubella is usually assessed by the detection of rubella virus-specific antibodies. Neutralization tests (NTs) are of highest diagnostic significance, because they measure the effectiveness of antibodies to block viral infection.

Objectives

The ability of rubella virus to cause a cytopathic effect (CPE) is limited. Therefore, most NT formats are based on immunoenzymatic techniques detecting viral proteins in infected cells. As Vero cells have been described to be susceptible to rubella virus infection with a more pronounced CPE, we aimed to develop an NT with these cells.

Methods

We compared rubella NTs with different infectivity readout methods (immunoenzymatic vs. CPE) using 42 human polyclonal samples with a known IgG ELISA concentration (IU/ml).

Results

We found a strong positive correlation between rubella virus-specific ELISA IgG units and neutralization titers determined with the two different NTs as well as between the two NT formats.

Conclusion

NT titer determination using CPE as a readout for infectivity is a reliable and promising method for rubella serology.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.