Zheng Lu, Xia Cui, Di Wang, Yi Zhang, Jiali Duan, Rong Zhao
{"title":"[2021 - 2023年北京市食源性蜡样芽孢杆菌毒力基因分布]。","authors":"Zheng Lu, Xia Cui, Di Wang, Yi Zhang, Jiali Duan, Rong Zhao","doi":"10.19813/j.cnki.weishengyanjiu.2025.04.014","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the pollution level and the carrying and distribution of virulence genes of foodborne Bacillus cereus in Beijing.</p><p><strong>Methods: </strong>The contamination levels of B. cereus in 805 food items sold in Beijing from 2021 to 2023 was examined and analyzed, and the 10 virulence genes of B. cereus were screened in the isolated stains by PCR. Meanwhile, the distribution of virulence genes related to the production of diarrhea and vomiting toxins was studied.</p><p><strong>Results: </strong>805 pieces of food sold in Beijing have an overall pollution level between 10-5.7×10~4 CFU/g, and no samples with a concentration greater than 10~5 CFU/g have been detected. A total of 37 virulence gene carrige patterns were identified, among which the non-hemolysin gene had the highest carriage rate of 100%(80/80), and the carrier rate of hemolysin BL genes is 65%(52/80). There are 16 strains carrying three non-hemolysin genes and three hemolysin genes simultaneously. The carrying rate of ces gene is 15.0%.</p><p><strong>Conclusion: </strong>The food contamination level of B. cereus in Beijing from 2021 to 2023 is relatively low, but it generally carries virulence genes.</p>","PeriodicalId":57744,"journal":{"name":"卫生研究","volume":"54 4","pages":"626-630"},"PeriodicalIF":0.0000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Distribution of virulence genes of foodborne Bacillus cereus in Beijing from 2021 to 2023].\",\"authors\":\"Zheng Lu, Xia Cui, Di Wang, Yi Zhang, Jiali Duan, Rong Zhao\",\"doi\":\"10.19813/j.cnki.weishengyanjiu.2025.04.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the pollution level and the carrying and distribution of virulence genes of foodborne Bacillus cereus in Beijing.</p><p><strong>Methods: </strong>The contamination levels of B. cereus in 805 food items sold in Beijing from 2021 to 2023 was examined and analyzed, and the 10 virulence genes of B. cereus were screened in the isolated stains by PCR. Meanwhile, the distribution of virulence genes related to the production of diarrhea and vomiting toxins was studied.</p><p><strong>Results: </strong>805 pieces of food sold in Beijing have an overall pollution level between 10-5.7×10~4 CFU/g, and no samples with a concentration greater than 10~5 CFU/g have been detected. A total of 37 virulence gene carrige patterns were identified, among which the non-hemolysin gene had the highest carriage rate of 100%(80/80), and the carrier rate of hemolysin BL genes is 65%(52/80). There are 16 strains carrying three non-hemolysin genes and three hemolysin genes simultaneously. The carrying rate of ces gene is 15.0%.</p><p><strong>Conclusion: </strong>The food contamination level of B. cereus in Beijing from 2021 to 2023 is relatively low, but it generally carries virulence genes.</p>\",\"PeriodicalId\":57744,\"journal\":{\"name\":\"卫生研究\",\"volume\":\"54 4\",\"pages\":\"626-630\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"卫生研究\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.04.014\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"卫生研究","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19813/j.cnki.weishengyanjiu.2025.04.014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Distribution of virulence genes of foodborne Bacillus cereus in Beijing from 2021 to 2023].
Objective: To investigate the pollution level and the carrying and distribution of virulence genes of foodborne Bacillus cereus in Beijing.
Methods: The contamination levels of B. cereus in 805 food items sold in Beijing from 2021 to 2023 was examined and analyzed, and the 10 virulence genes of B. cereus were screened in the isolated stains by PCR. Meanwhile, the distribution of virulence genes related to the production of diarrhea and vomiting toxins was studied.
Results: 805 pieces of food sold in Beijing have an overall pollution level between 10-5.7×10~4 CFU/g, and no samples with a concentration greater than 10~5 CFU/g have been detected. A total of 37 virulence gene carrige patterns were identified, among which the non-hemolysin gene had the highest carriage rate of 100%(80/80), and the carrier rate of hemolysin BL genes is 65%(52/80). There are 16 strains carrying three non-hemolysin genes and three hemolysin genes simultaneously. The carrying rate of ces gene is 15.0%.
Conclusion: The food contamination level of B. cereus in Beijing from 2021 to 2023 is relatively low, but it generally carries virulence genes.
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