免疫细胞聚集体的软物质力学。

IF 3.5 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Journal of The Royal Society Interface Pub Date : 2025-07-01 Epub Date: 2025-07-23 DOI:10.1098/rsif.2025.0231
Shohreh Askari, Guillem Saldo Rubio, Anagha Datar, Heidi Harjunpää, Susanna C Fagerholm, Matilda Backholm
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引用次数: 0

摘要

t细胞是白细胞的一个重要子集,在免疫系统中起着核心作用。当t细胞结合抗原时,它会导致细胞活化并诱导免疫反应。如果t细胞在体内或体外被抗原激活,它们会形成多细胞聚集体。这些簇的机械特性提供了不同t细胞激活途径的宝贵信息。此外,集合力学捕获了t细胞如何受到机械力的影响,并在更大的集合(如淋巴结和肿瘤)中相互作用。然而,对集体t细胞粘附和细胞活化后的机制的理解目前还缺乏。探索易碎的和微小的活样本的力学在实验上是具有挑战性的。在这里,微管力传感器技术被用来拉伸t细胞聚集体,并直接测量它们的杨氏模量和极限拉伸强度。建立了一个机制模型,以关联中尺度多细胞聚集体的刚度如何从集群内单个微观细胞的机械响应中产生。我们展示了聚集体弹性如何受到不同激活剂的影响,并将其与细胞中不同的激活途径联系起来。我们对多细胞t细胞聚集体的软物质力学研究有助于我们理解免疫细胞激活背后的生物学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Soft matter mechanics of immune cell aggregates.

Soft matter mechanics of immune cell aggregates.

Soft matter mechanics of immune cell aggregates.

Soft matter mechanics of immune cell aggregates.

T-cells are a crucial subset of white blood cells that play a central role in the immune system. When T-cells bind antigens, it leads to cell activation and the induction of an immune response. If T-cells are activated by antigens in vivo or artificially in vitro, they form multicellular aggregates. The mechanical properties of such clusters provide valuable information on different T-cell activation pathways. Furthermore, the aggregate mechanics capture how T-cells are affected by mechanical forces and interact within larger conglomerates, such as lymph nodes and tumours. However, an understanding of collective T-cell adhesion and mechanics following cell activation is currently lacking. Probing the mechanics of fragile and microscopically small living samples is experimentally challenging. Here, the micropipette force sensor technique was used to stretch T-cell aggregates and directly measure their Young's modulus and ultimate tensile strength. A mechanistic model was developed to correlate how the stiffness of the mesoscale multicellular aggregate emerges from the mechanical response of the individual microscopic cells within the cluster. We show how the aggregate elasticity is affected by different activators and relate this to different activation pathways in the cells. Our soft matter mechanics study of multicellular T-cell aggregates contributes to our understanding of the biology behind immune cell activation.

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来源期刊
Journal of The Royal Society Interface
Journal of The Royal Society Interface 综合性期刊-综合性期刊
CiteScore
7.10
自引率
2.60%
发文量
234
审稿时长
2.5 months
期刊介绍: J. R. Soc. Interface welcomes articles of high quality research at the interface of the physical and life sciences. It provides a high-quality forum to publish rapidly and interact across this boundary in two main ways: J. R. Soc. Interface publishes research applying chemistry, engineering, materials science, mathematics and physics to the biological and medical sciences; it also highlights discoveries in the life sciences of relevance to the physical sciences. Both sides of the interface are considered equally and it is one of the only journals to cover this exciting new territory. J. R. Soc. Interface welcomes contributions on a diverse range of topics, including but not limited to; biocomplexity, bioengineering, bioinformatics, biomaterials, biomechanics, bionanoscience, biophysics, chemical biology, computer science (as applied to the life sciences), medical physics, synthetic biology, systems biology, theoretical biology and tissue engineering.
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