真菌膜的原位界面剪切流变学。

IF 3.5 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Journal of The Royal Society Interface Pub Date : 2025-07-01 Epub Date: 2025-07-23 DOI:10.1098/rsif.2025.0058
Ciatta Wobill, Samuel Steffen, Daniel Macken, Peter Fischer, Patrick Alberto Rühs
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引用次数: 0

摘要

了解真菌的动态膜形成对于设计和创造工程活真菌材料是必不可少的。然而,传统的生长测量往往忽略了真菌生长的三维性,因此只能提供关于瞬时力学特性的稀缺信息。在这项研究中,我们使用界面剪切流变学(ISR)来研究真菌膜的动态形成。利用ISR技术可以通过测定膜的粘弹性来识别真菌生长的滞后期、指数期和平稳期。我们观察到孢子萌发前2小时贮藏模量增加,这很可能是由于孢子在膨胀和萌发过程中被吸收和代谢副产物引起的。通过菌落直径测量、分光光度法和ISR可以看出,改变液体培养基中的糖浓度可以延迟萌发,同时提高真菌的指数繁殖速度。此外,我们能够通过增加界面模量来观察薄膜的形成,作为生物量的度量。结合分光光度法和菌落直径测量,ISR提供了3天内瞬态成膜的完整图像,通过提供非破坏性的生物量定性测量和成膜强度定量测量,揭示了萌发、指数生长和固定阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In situ interfacial shear rheology of fungal films.

Understanding the dynamic film formation of fungi is essential for designing and creating engineered living fungal materials. However, conventional growth measurements often neglect the three-dimensionality of fungal growth and thus only provide scarce information about the transient mechanical properties. In this study, we use interfacial shear rheology (ISR) to investigate the dynamic formation of fungal films. By using ISR we can identify the lag, exponential and stationary phases of fungal growth by measuring the film viscoelasticity. We observed an increase in storage modulus after 2 h before spore germination, most likely caused by the absorption of spores and metabolic by-products during swelling and germination. Modifying the sugar concentration in the liquid growth media allowed for delayed germination while enhancing the exponential propagation speed of fungi, as visible through colony diameter measurements, spectrophotometry and ISR. Furthermore, we were able to observe the film formation through an increase in interfacial moduli as a measure of biomass. In combination with spectrophotometry and colony diameter measurements, ISR provides a complete picture of the transient film formation over 3 days, shedding light on the germination, exponential growth and stationary phase by providing non-destructive, qualitative measures for biomass and quantitative measures for film strength.

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来源期刊
Journal of The Royal Society Interface
Journal of The Royal Society Interface 综合性期刊-综合性期刊
CiteScore
7.10
自引率
2.60%
发文量
234
审稿时长
2.5 months
期刊介绍: J. R. Soc. Interface welcomes articles of high quality research at the interface of the physical and life sciences. It provides a high-quality forum to publish rapidly and interact across this boundary in two main ways: J. R. Soc. Interface publishes research applying chemistry, engineering, materials science, mathematics and physics to the biological and medical sciences; it also highlights discoveries in the life sciences of relevance to the physical sciences. Both sides of the interface are considered equally and it is one of the only journals to cover this exciting new territory. J. R. Soc. Interface welcomes contributions on a diverse range of topics, including but not limited to; biocomplexity, bioengineering, bioinformatics, biomaterials, biomechanics, bionanoscience, biophysics, chemical biology, computer science (as applied to the life sciences), medical physics, synthetic biology, systems biology, theoretical biology and tissue engineering.
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