Cheng Zong, Longxin Wang, Jie Zhao, Zhihao Dong, Junfeng Li, Xianjun Yuan, Chengti Xu, Tao Shao
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引用次数: 0
摘要
目的:构建并分析青贮源性乳酸菌(LAB)菌群的代谢组学。方法与结果:从不同青贮中分离LAB菌株,采用自下而上的方法构建不同生物多样性类型的LAB群体。共分离到菌株192株,其中9株为代表性菌株。随后,构建了5个不同生物多样性水平(4 ~ 8种)的LAB群落。这一构建基于两个关键因素:菌株之间不存在拮抗作用以及它们的酸化能力,以pH值(3.60-3.71)、乳酸(23.6-26.4 g L-1)和乙酸(6.68-9.04 g L-1)为特征。进一步的发酵实验表明,5个发酵菌群的发酵性能均优于苜蓿发酵青汁。采用非靶向代谢组学方法分析LAB菌群中代谢物和代谢途径的差异。值得注意的是,6种氨基酸和5种b族维生素在5个乳酸菌群中存在差异,乳酸菌群的关键代谢途径与氨基酸和维生素有关。结论:构建了5个具有高酸化能力的LAB联合体。乳酸菌群间的主要代谢差异是6种氨基酸和5种b族维生素的水平。
Construction and metabolomics of silage-derived lactic acid bacteria-based consortia.
Aims: The objective of this study was to construct and analyze the metabolomics of silage-derived lactic acid bacteria (LAB)-based consortia.
Methods and results: The LAB strains were isolated from various silages, and LAB-based consortia with different types of biodiversity were constructed by a bottom-up approach. Two hundred and ninety-two LAB strains were isolated, nine of which were selected as representative strains. Subsequently, five LAB consortia with diverse biodiversity levels (ranging from 4 to 8 species) were constructed. This construction was based on two key factors: the absence of antagonism among strains and their acidification capabilities, which were characterized by pH values (3.60-3.71), lactate (23.6-26.4 g L-1), and acetate (6.68-9.04 g L-1). Further fermentation experiments demonstrated that the five LAB consortia exhibited superior performance compared to alfalfa fermented green juice. Untargeted metabolomics was employed to analyze the differentially abundant metabolites and metabolic pathways of LAB consortia. Notably, 6 amino acids and 5 B-group vitamins varied among five LAB consortia, and key metabolic pathways were related to amino acids and vitamins for the LAB consortia.
Conclusions: Five LAB consortia with high acidification ability were constructed. The primary metabolic differences observed among the LAB consortia were the levels of 6 amino acids and 5 B-group vitamins.
期刊介绍:
Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.