{"title":"抗基质干扰抗维生素A单克隆抗体的制备及其在定量免疫层析分析中的应用。","authors":"Jialin Hu, Lingling Guo, Shanshan Song, Liqiang Liu, Xinxin Xu, Hua Kuang, Chuanlai Xu, Liguang Xu","doi":"10.1007/s00604-025-07379-4","DOIUrl":null,"url":null,"abstract":"<p><p>Vitamin A (VA) deficiency can lead to abnormal immune system function and jeopardize health. Therefore, a simple and specific method for determining VA is needed. We analyzed the structure of hapten for the preparation of an anti-VA monoclonal antibody (mAb), assisted by computer simulation. The solution's organic solvent content was optimized during mAbs screening, taking into account its effect on antibody performance. Colloidal gold immunochromatographic assay (CGIA) strips were developed and applied to the detection of VA in infant formula, cheese, and VA dietary supplements, utilizing the prepared mAb that exhibits tolerance to 10% methanol. The visual and calculated limit of detection for infant formula, cheese, VA dietary supplements, and human plasma were 100, 50, 50 mg/kg, and 100 μg/mL and 15.06, 15.21, 27.47 mg/kg, and 20.23 μg/mL, respectively. The recoveries of CGIA strips for the detection of VA ranged from 93.67% to 102.68%. This agrees well with the liquid chromatography-tandem mass spectrometry results. Therefore, the CGIA strips could fulfill the needs for the determination of VA in real samples.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"508"},"PeriodicalIF":5.3000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation of an anti-vitamin A monoclonal antibody resistant to matrix interference and its application in quantitative immunochromatographic analysis.\",\"authors\":\"Jialin Hu, Lingling Guo, Shanshan Song, Liqiang Liu, Xinxin Xu, Hua Kuang, Chuanlai Xu, Liguang Xu\",\"doi\":\"10.1007/s00604-025-07379-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Vitamin A (VA) deficiency can lead to abnormal immune system function and jeopardize health. Therefore, a simple and specific method for determining VA is needed. We analyzed the structure of hapten for the preparation of an anti-VA monoclonal antibody (mAb), assisted by computer simulation. The solution's organic solvent content was optimized during mAbs screening, taking into account its effect on antibody performance. Colloidal gold immunochromatographic assay (CGIA) strips were developed and applied to the detection of VA in infant formula, cheese, and VA dietary supplements, utilizing the prepared mAb that exhibits tolerance to 10% methanol. The visual and calculated limit of detection for infant formula, cheese, VA dietary supplements, and human plasma were 100, 50, 50 mg/kg, and 100 μg/mL and 15.06, 15.21, 27.47 mg/kg, and 20.23 μg/mL, respectively. The recoveries of CGIA strips for the detection of VA ranged from 93.67% to 102.68%. This agrees well with the liquid chromatography-tandem mass spectrometry results. Therefore, the CGIA strips could fulfill the needs for the determination of VA in real samples.</p>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 8\",\"pages\":\"508\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s00604-025-07379-4\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00604-025-07379-4","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Preparation of an anti-vitamin A monoclonal antibody resistant to matrix interference and its application in quantitative immunochromatographic analysis.
Vitamin A (VA) deficiency can lead to abnormal immune system function and jeopardize health. Therefore, a simple and specific method for determining VA is needed. We analyzed the structure of hapten for the preparation of an anti-VA monoclonal antibody (mAb), assisted by computer simulation. The solution's organic solvent content was optimized during mAbs screening, taking into account its effect on antibody performance. Colloidal gold immunochromatographic assay (CGIA) strips were developed and applied to the detection of VA in infant formula, cheese, and VA dietary supplements, utilizing the prepared mAb that exhibits tolerance to 10% methanol. The visual and calculated limit of detection for infant formula, cheese, VA dietary supplements, and human plasma were 100, 50, 50 mg/kg, and 100 μg/mL and 15.06, 15.21, 27.47 mg/kg, and 20.23 μg/mL, respectively. The recoveries of CGIA strips for the detection of VA ranged from 93.67% to 102.68%. This agrees well with the liquid chromatography-tandem mass spectrometry results. Therefore, the CGIA strips could fulfill the needs for the determination of VA in real samples.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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