David M. Jaramillo, Lisa M. Bauman, Carlos Henrique Paiva Camisa Nova, Lais Lima, Edzard van Santen
{"title":"采血管对牛血液中血浆、红细胞和血清碳氮同位素组成干扰最小","authors":"David M. Jaramillo, Lisa M. Bauman, Carlos Henrique Paiva Camisa Nova, Lais Lima, Edzard van Santen","doi":"10.1002/rcm.10109","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Rationale</h3>\n \n <p>Stable isotopes can be useful for tracking diet composition and other physiological processes in ruminants. These techniques rely on the fact that tissues directly reflect the animal's diet. Blood is a dynamic tissue that can be separated into serum, plasma, and red blood cell (RBC) components. Numerous collection tubes for blood are commercially available. No studies have compared the isotopic composition from cattle blood as affected by collection tube. This study compared commercially available serum and plasma tubes and evaluated how they may affect δ<sup>13</sup>C, δ<sup>15</sup>N, and C and N concentrations from blood in lactating dairy cattle.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>For plasma and RBC, treatments were five collection tubes containing different anticoagulants: dipotassium ethylenediaminetetraacetic acid (K2EDTA), tripotassium EDTA (K3EDTA), NaF/K oxalate, Li heparin (LiHep), and Na heparin (NaHep). For serum analysis, the three collection tubes were a RedTop (contained no coagulating agent) and TigerTop and Red+CAT, which both contained clot activator and serum separator gel, each made by two different manufacturers. All samples were collected via tail venipuncture in cows. All samples were processed, freeze dried, and analyzed in triplicate for δ<sup>13</sup>C, δ<sup>15</sup>N, and C and N.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>While mean differences exist on the δ<sup>13</sup>C and δ<sup>15</sup>N from plasma and red blood cells, differences were minimal. Clot activator gels in serum tubes did not affect the δ<sup>13</sup>C and δ<sup>15</sup>N composition. This study showed that, while isotopic values may differ slightly from different blood collection tubes, these differences were often small, which may have little impact on the interpretation of research results when serum or RBC are analyzed. The isotopic composition of C and N concentrations of serum was not affected by the three tubes used within this study. Researchers will have to understand these differences and decide whether or not these may impact the objectives and the results of a given study.</p>\n </section>\n </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 21","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Blood Collection Tube has Minimal Interference on the Carbon and Nitrogen Isotope Composition of Plasma, Red Blood Cells, and Serum in Cow Blood\",\"authors\":\"David M. Jaramillo, Lisa M. Bauman, Carlos Henrique Paiva Camisa Nova, Lais Lima, Edzard van Santen\",\"doi\":\"10.1002/rcm.10109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Rationale</h3>\\n \\n <p>Stable isotopes can be useful for tracking diet composition and other physiological processes in ruminants. These techniques rely on the fact that tissues directly reflect the animal's diet. Blood is a dynamic tissue that can be separated into serum, plasma, and red blood cell (RBC) components. Numerous collection tubes for blood are commercially available. No studies have compared the isotopic composition from cattle blood as affected by collection tube. This study compared commercially available serum and plasma tubes and evaluated how they may affect δ<sup>13</sup>C, δ<sup>15</sup>N, and C and N concentrations from blood in lactating dairy cattle.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>For plasma and RBC, treatments were five collection tubes containing different anticoagulants: dipotassium ethylenediaminetetraacetic acid (K2EDTA), tripotassium EDTA (K3EDTA), NaF/K oxalate, Li heparin (LiHep), and Na heparin (NaHep). For serum analysis, the three collection tubes were a RedTop (contained no coagulating agent) and TigerTop and Red+CAT, which both contained clot activator and serum separator gel, each made by two different manufacturers. All samples were collected via tail venipuncture in cows. All samples were processed, freeze dried, and analyzed in triplicate for δ<sup>13</sup>C, δ<sup>15</sup>N, and C and N.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>While mean differences exist on the δ<sup>13</sup>C and δ<sup>15</sup>N from plasma and red blood cells, differences were minimal. Clot activator gels in serum tubes did not affect the δ<sup>13</sup>C and δ<sup>15</sup>N composition. This study showed that, while isotopic values may differ slightly from different blood collection tubes, these differences were often small, which may have little impact on the interpretation of research results when serum or RBC are analyzed. The isotopic composition of C and N concentrations of serum was not affected by the three tubes used within this study. Researchers will have to understand these differences and decide whether or not these may impact the objectives and the results of a given study.</p>\\n </section>\\n </div>\",\"PeriodicalId\":225,\"journal\":{\"name\":\"Rapid Communications in Mass Spectrometry\",\"volume\":\"39 21\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rapid Communications in Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/rcm.10109\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rapid Communications in Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/rcm.10109","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Blood Collection Tube has Minimal Interference on the Carbon and Nitrogen Isotope Composition of Plasma, Red Blood Cells, and Serum in Cow Blood
Rationale
Stable isotopes can be useful for tracking diet composition and other physiological processes in ruminants. These techniques rely on the fact that tissues directly reflect the animal's diet. Blood is a dynamic tissue that can be separated into serum, plasma, and red blood cell (RBC) components. Numerous collection tubes for blood are commercially available. No studies have compared the isotopic composition from cattle blood as affected by collection tube. This study compared commercially available serum and plasma tubes and evaluated how they may affect δ13C, δ15N, and C and N concentrations from blood in lactating dairy cattle.
Methods
For plasma and RBC, treatments were five collection tubes containing different anticoagulants: dipotassium ethylenediaminetetraacetic acid (K2EDTA), tripotassium EDTA (K3EDTA), NaF/K oxalate, Li heparin (LiHep), and Na heparin (NaHep). For serum analysis, the three collection tubes were a RedTop (contained no coagulating agent) and TigerTop and Red+CAT, which both contained clot activator and serum separator gel, each made by two different manufacturers. All samples were collected via tail venipuncture in cows. All samples were processed, freeze dried, and analyzed in triplicate for δ13C, δ15N, and C and N.
Conclusions
While mean differences exist on the δ13C and δ15N from plasma and red blood cells, differences were minimal. Clot activator gels in serum tubes did not affect the δ13C and δ15N composition. This study showed that, while isotopic values may differ slightly from different blood collection tubes, these differences were often small, which may have little impact on the interpretation of research results when serum or RBC are analyzed. The isotopic composition of C and N concentrations of serum was not affected by the three tubes used within this study. Researchers will have to understand these differences and decide whether or not these may impact the objectives and the results of a given study.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
