Rong Er Lim, Syahirah Faraheen Kabir Ahmad, Gobi Kanadasan and Vel Murugan Vadivelu
{"title":"好氧颗粒中聚羟基烷酸酯提取方法的生命周期评价","authors":"Rong Er Lim, Syahirah Faraheen Kabir Ahmad, Gobi Kanadasan and Vel Murugan Vadivelu","doi":"10.1039/D5EW00335K","DOIUrl":null,"url":null,"abstract":"<p >Aerobic granules were cultivated to accumulate polyhydroxyalkanoate (PHA) using palm oil mill effluent (POME) in a sequencing batch reactor (SBR). Five PHA extraction methods using different cell digestion chemicals, namely, sodium hypochlorite, acetone, sodium chloride, and sodium hydroxide, and without chemicals (control), were studied to identify the most environmentally friendly method and the most contributing factor within the methods. Method 1 (sodium hypochlorite) and method 2 (acetone) provided PHA yields of approximately 85% cell dry weight (CDW). The PHA that was recovered was a P3(HB-<em>co</em>-HV) co-polymer. SimaPro software version 8.5, developed by PRé Sustainability, was used to analyze the impact of the extraction methods on seven selected impact categories. Life cycle impact assessment of each of the methods for the production of 1 kg of dried PHA indicated that methods 1 (sodium hypochlorite) and 2 (acetone) had the lowest impact on all impact categories studied. It was found that the cultivation process of aerobic granules, electricity consumption, and usage of trichloromethane during PHA extraction were major contributors to all the impact categories.</p>","PeriodicalId":75,"journal":{"name":"Environmental Science: Water Research & Technology","volume":" 8","pages":" 1966-1976"},"PeriodicalIF":3.1000,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Life cycle assessment on methods for polyhydroxyalkanoate extraction from aerobic granules\",\"authors\":\"Rong Er Lim, Syahirah Faraheen Kabir Ahmad, Gobi Kanadasan and Vel Murugan Vadivelu\",\"doi\":\"10.1039/D5EW00335K\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Aerobic granules were cultivated to accumulate polyhydroxyalkanoate (PHA) using palm oil mill effluent (POME) in a sequencing batch reactor (SBR). Five PHA extraction methods using different cell digestion chemicals, namely, sodium hypochlorite, acetone, sodium chloride, and sodium hydroxide, and without chemicals (control), were studied to identify the most environmentally friendly method and the most contributing factor within the methods. Method 1 (sodium hypochlorite) and method 2 (acetone) provided PHA yields of approximately 85% cell dry weight (CDW). The PHA that was recovered was a P3(HB-<em>co</em>-HV) co-polymer. SimaPro software version 8.5, developed by PRé Sustainability, was used to analyze the impact of the extraction methods on seven selected impact categories. Life cycle impact assessment of each of the methods for the production of 1 kg of dried PHA indicated that methods 1 (sodium hypochlorite) and 2 (acetone) had the lowest impact on all impact categories studied. It was found that the cultivation process of aerobic granules, electricity consumption, and usage of trichloromethane during PHA extraction were major contributors to all the impact categories.</p>\",\"PeriodicalId\":75,\"journal\":{\"name\":\"Environmental Science: Water Research & Technology\",\"volume\":\" 8\",\"pages\":\" 1966-1976\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-06-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Science: Water Research & Technology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ew/d5ew00335k\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science: Water Research & Technology","FirstCategoryId":"93","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ew/d5ew00335k","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Life cycle assessment on methods for polyhydroxyalkanoate extraction from aerobic granules
Aerobic granules were cultivated to accumulate polyhydroxyalkanoate (PHA) using palm oil mill effluent (POME) in a sequencing batch reactor (SBR). Five PHA extraction methods using different cell digestion chemicals, namely, sodium hypochlorite, acetone, sodium chloride, and sodium hydroxide, and without chemicals (control), were studied to identify the most environmentally friendly method and the most contributing factor within the methods. Method 1 (sodium hypochlorite) and method 2 (acetone) provided PHA yields of approximately 85% cell dry weight (CDW). The PHA that was recovered was a P3(HB-co-HV) co-polymer. SimaPro software version 8.5, developed by PRé Sustainability, was used to analyze the impact of the extraction methods on seven selected impact categories. Life cycle impact assessment of each of the methods for the production of 1 kg of dried PHA indicated that methods 1 (sodium hypochlorite) and 2 (acetone) had the lowest impact on all impact categories studied. It was found that the cultivation process of aerobic granules, electricity consumption, and usage of trichloromethane during PHA extraction were major contributors to all the impact categories.
期刊介绍:
Environmental Science: Water Research & Technology seeks to showcase high quality research about fundamental science, innovative technologies, and management practices that promote sustainable water.