{"title":"hplc - ms / ms引导分离菊花中具有抗炎活性的愈创木酚烷型倍半萜","authors":"Xinyue Li, Yanfen Cai, Yunshuang Hu, Limei Miu, Yanyu Zhang, Shiyun Huang, Min Wei, Qing Ma, Zhongqiu Liu, Hua Zhou and Peng Wu","doi":"10.1039/D5RA03586D","DOIUrl":null,"url":null,"abstract":"<p >Four previously undescribed compounds, including three guaiacane-type sesquiterpenoids (<strong>1–3</strong>) and one oleanane-type triterpenoid (<strong>4</strong>), along with seven known sesquiterpenoids, were isolated from the aerial parts of <em>Chrysanthemum indicum</em> using LC-MS/MS-guided fractionation. Structures were elucidated by IR, UV, HR-ESI-MS, and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory activity was evaluated in LPS-stimulated RAW264.7 cells with NF-κB translocation by high-content imaging (HCI). Compound <strong>2</strong> reduced NF-κB translocation (IC<small><sub>50</sub></small> = 9.70 μM) without cytotoxicity at 20 μM. Compounds <strong>8</strong> and <strong>9</strong> showed potent activity (IC<small><sub>50</sub></small> = 2.04 and 1.21 μM, respectively) and no cytotoxicity at 6.25 μM.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":" 32","pages":" 26173-26183"},"PeriodicalIF":4.6000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra03586d?page=search","citationCount":"0","resultStr":"{\"title\":\"LC-MS/MS-guided separation of guaiacolane-type sesquiterpenes with anti-inflammatory activities from Chrysanthemum indicum†\",\"authors\":\"Xinyue Li, Yanfen Cai, Yunshuang Hu, Limei Miu, Yanyu Zhang, Shiyun Huang, Min Wei, Qing Ma, Zhongqiu Liu, Hua Zhou and Peng Wu\",\"doi\":\"10.1039/D5RA03586D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Four previously undescribed compounds, including three guaiacane-type sesquiterpenoids (<strong>1–3</strong>) and one oleanane-type triterpenoid (<strong>4</strong>), along with seven known sesquiterpenoids, were isolated from the aerial parts of <em>Chrysanthemum indicum</em> using LC-MS/MS-guided fractionation. Structures were elucidated by IR, UV, HR-ESI-MS, and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory activity was evaluated in LPS-stimulated RAW264.7 cells with NF-κB translocation by high-content imaging (HCI). Compound <strong>2</strong> reduced NF-κB translocation (IC<small><sub>50</sub></small> = 9.70 μM) without cytotoxicity at 20 μM. Compounds <strong>8</strong> and <strong>9</strong> showed potent activity (IC<small><sub>50</sub></small> = 2.04 and 1.21 μM, respectively) and no cytotoxicity at 6.25 μM.</p>\",\"PeriodicalId\":102,\"journal\":{\"name\":\"RSC Advances\",\"volume\":\" 32\",\"pages\":\" 26173-26183\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra03586d?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Advances\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra03586d\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Advances","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra03586d","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
LC-MS/MS-guided separation of guaiacolane-type sesquiterpenes with anti-inflammatory activities from Chrysanthemum indicum†
Four previously undescribed compounds, including three guaiacane-type sesquiterpenoids (1–3) and one oleanane-type triterpenoid (4), along with seven known sesquiterpenoids, were isolated from the aerial parts of Chrysanthemum indicum using LC-MS/MS-guided fractionation. Structures were elucidated by IR, UV, HR-ESI-MS, and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory activity was evaluated in LPS-stimulated RAW264.7 cells with NF-κB translocation by high-content imaging (HCI). Compound 2 reduced NF-κB translocation (IC50 = 9.70 μM) without cytotoxicity at 20 μM. Compounds 8 and 9 showed potent activity (IC50 = 2.04 and 1.21 μM, respectively) and no cytotoxicity at 6.25 μM.
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An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.
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