中华环虫热休克蛋白90的鉴定与表征及其在热感染和副溶血性弧菌感染下的功能

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-07-21 DOI:10.1016/j.genrep.2025.102303

Dehui Sun , Lisha Wei , Ruyan Yi , Yujuan Hong , Yuwei Wang , Wenwen Dong , Fengjuan Jiang , Qing Nie , Xiuke Ouyang

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引用次数: 0

摘要

HSP90是热休克蛋白超家族的一员，在多种应激反应中发挥重要作用。在本研究中，我们从Cyclina sinensis基因组数据库中鉴定了CsHSP90。CsHSP90编码序列全长2184 bp，编码727个氨基酸，分子量为83.67 kDa，等电点为4.85。分析结果表明，CsHSP90是预测的亲水性蛋白，主要定位于细胞质中。基序分析、三维结构建模和氨基酸序列比对结果表明，HSP90具有高度保守性。系统发育分析表明，CsHSP90与双壳菌hsp90聚类。qRT-PCR分析结果显示，CsHSP90在所有组织中均检测到，主要在内收肌中表达。在高温胁迫和副溶血性弧菌感染下表达量增加。这些结果为揭示CsHsp90在应激反应中的分子功能积累了基础数据。

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Identification and characterization of heat shock protein 90 in Cyclina sinensis and its function under thermal and Vibrio parahaemolyticus infection

HSP90 is a member of heat shock protein superfamily and plays an important role in response to various stressors. In this study, we identified the CsHSP90 from genomic databases of Cyclina sinensis. The full length of the CsHSP90 coding sequence (CDS) was 2184 bp and coding 727 amino acids with an estimated molecular mass of 83.67 kDa and isoelectric point of 4.85. The analysis result showed that CsHSP90 was a predicted hydrophilic protein and mainly localized in the cytoplasm. The results of motif analysis, three-dimensional structural modeling and amino acid sequence alignment suggested that HSP90 were highly conserved. Phylogenetic analysis indicated that CsHSP90 was clustered with HSP90s of Bivalve. The qRT-PCR analysis results showed that CsHSP90 was detected in all the tissue and primarily expressed in the adductor. The expression was increased under high temperature stress and Vibrio parahaemolyticus infection. These results accumulate basic data for revealing the molecular function of CsHsp90 in stress response.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.