Davide Gerna, Fabio Candotto Carniel, Louise Colville, Johanna Wagner, Daniela Festi
{"title":"除草剂配方损害了榛花粉的体外性能:天然的并不安全。","authors":"Davide Gerna, Fabio Candotto Carniel, Louise Colville, Johanna Wagner, Daniela Festi","doi":"10.1016/j.envpol.2025.126870","DOIUrl":null,"url":null,"abstract":"The progamic phase from pollination to fertilisation is a critical stage of plant life cycle that is vulnerable to chemical exposure and requires controlled production of reactive oxygen species (ROS). Herbicide formulations are integral to agriculture, horticulture, and forestry, though surprisingly little is known about pollen exposure to commercial herbicide formulations, especially for non-target (i.e., non-weed) plants. Here, we compared the effects of a synthetic formulation of glyphosate (Roundup) and a natural formulation of pelargonic acid (Herbistop) on the <em>in vitro</em> performance of hazel (<em>Corylus avellana</em> L.) pollen. At equal pH values, Herbistop (half-maximal inhibitory concentration [IC50] 10.7-11.2 mg acid equivalents [a.e.] L<sup>-1</sup>) restricted pollen tube elongation at concentrations one order of magnitude lower than Roundup (IC50 332.0-410.0 mg a.e. L<sup>-1</sup>) and in a narrower range. Pollen staining with triphenyl tetrazolium chloride confirmed the toxicity of both formulations, with Herbistop compromising viability more severely. At respective IC50 values, both herbicide formulations curtailed the production of the ROS hydrogen peroxide during pollen tube elongation, impairing viability, and slowing down <em>in vitro</em> germination. Overall, our <em>in vitro</em> evidence shows that the natural formulation, Herbistop, was more hazardous for hazel pollen performance than the synthetic formulation, RoundUp. This highlights the common misconception that usage of natural herbicide formulations, rather than synthetic, is safer for non-target plants, other non-target organisms, and the environment. We propose that pollen performance is an additional toxicological endpoint of all herbicide formulations that requires special surveillance in gardens, woodlands, and agroecosystems.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"25 1","pages":""},"PeriodicalIF":7.6000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Herbicide formulations impair in vitro performance of hazel pollen: natural is not safer.\",\"authors\":\"Davide Gerna, Fabio Candotto Carniel, Louise Colville, Johanna Wagner, Daniela Festi\",\"doi\":\"10.1016/j.envpol.2025.126870\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The progamic phase from pollination to fertilisation is a critical stage of plant life cycle that is vulnerable to chemical exposure and requires controlled production of reactive oxygen species (ROS). Herbicide formulations are integral to agriculture, horticulture, and forestry, though surprisingly little is known about pollen exposure to commercial herbicide formulations, especially for non-target (i.e., non-weed) plants. Here, we compared the effects of a synthetic formulation of glyphosate (Roundup) and a natural formulation of pelargonic acid (Herbistop) on the <em>in vitro</em> performance of hazel (<em>Corylus avellana</em> L.) pollen. At equal pH values, Herbistop (half-maximal inhibitory concentration [IC50] 10.7-11.2 mg acid equivalents [a.e.] L<sup>-1</sup>) restricted pollen tube elongation at concentrations one order of magnitude lower than Roundup (IC50 332.0-410.0 mg a.e. L<sup>-1</sup>) and in a narrower range. Pollen staining with triphenyl tetrazolium chloride confirmed the toxicity of both formulations, with Herbistop compromising viability more severely. At respective IC50 values, both herbicide formulations curtailed the production of the ROS hydrogen peroxide during pollen tube elongation, impairing viability, and slowing down <em>in vitro</em> germination. Overall, our <em>in vitro</em> evidence shows that the natural formulation, Herbistop, was more hazardous for hazel pollen performance than the synthetic formulation, RoundUp. This highlights the common misconception that usage of natural herbicide formulations, rather than synthetic, is safer for non-target plants, other non-target organisms, and the environment. We propose that pollen performance is an additional toxicological endpoint of all herbicide formulations that requires special surveillance in gardens, woodlands, and agroecosystems.\",\"PeriodicalId\":311,\"journal\":{\"name\":\"Environmental Pollution\",\"volume\":\"25 1\",\"pages\":\"\"},\"PeriodicalIF\":7.6000,\"publicationDate\":\"2025-07-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Pollution\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.envpol.2025.126870\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.envpol.2025.126870","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Herbicide formulations impair in vitro performance of hazel pollen: natural is not safer.
The progamic phase from pollination to fertilisation is a critical stage of plant life cycle that is vulnerable to chemical exposure and requires controlled production of reactive oxygen species (ROS). Herbicide formulations are integral to agriculture, horticulture, and forestry, though surprisingly little is known about pollen exposure to commercial herbicide formulations, especially for non-target (i.e., non-weed) plants. Here, we compared the effects of a synthetic formulation of glyphosate (Roundup) and a natural formulation of pelargonic acid (Herbistop) on the in vitro performance of hazel (Corylus avellana L.) pollen. At equal pH values, Herbistop (half-maximal inhibitory concentration [IC50] 10.7-11.2 mg acid equivalents [a.e.] L-1) restricted pollen tube elongation at concentrations one order of magnitude lower than Roundup (IC50 332.0-410.0 mg a.e. L-1) and in a narrower range. Pollen staining with triphenyl tetrazolium chloride confirmed the toxicity of both formulations, with Herbistop compromising viability more severely. At respective IC50 values, both herbicide formulations curtailed the production of the ROS hydrogen peroxide during pollen tube elongation, impairing viability, and slowing down in vitro germination. Overall, our in vitro evidence shows that the natural formulation, Herbistop, was more hazardous for hazel pollen performance than the synthetic formulation, RoundUp. This highlights the common misconception that usage of natural herbicide formulations, rather than synthetic, is safer for non-target plants, other non-target organisms, and the environment. We propose that pollen performance is an additional toxicological endpoint of all herbicide formulations that requires special surveillance in gardens, woodlands, and agroecosystems.
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.