Manar Fouli Gaber Ibrahim, Elshymaa A Abdel-Hakeem, Heba A Shawky, Soha Abdelkawy Abdelwahab, Sahar A Mokhemer
{"title":"局部与全身外泌体给药对大鼠睾丸缺血再灌注损伤模型双睾丸的影响:对血睾丸屏障的研究。","authors":"Manar Fouli Gaber Ibrahim, Elshymaa A Abdel-Hakeem, Heba A Shawky, Soha Abdelkawy Abdelwahab, Sahar A Mokhemer","doi":"10.1080/21688370.2025.2532195","DOIUrl":null,"url":null,"abstract":"<p><p>Exosomes<sup>,</sup> (EXs) competence in reproductive medicine has been proven, particularly in cases of ischemia reperfusion (IR) injury models. Testicular torsion is a major clinical concern affecting male fertility and results in ischemic reperfusion injury. To the best of our knowledge, up to date, comparative studies using different routes of EXs application have not been investigated. Thus, we aimed to investigate the effect of local versus systemic EXs administration on both testes; ipsilaterally and contralaterally and to explore the involved underlying mechanisms. Rats were allocated into four groups; the control, the ischemia reperfusion group (IR group) in which unilateral testicular IR was performed, then rats were subdivided equally into two subgroups; IR-ipsi group; in which the ipsilateral testes were investigated and the IR-contra group in which the contralateral testes were examined. Group III and group IV in which unilateral IR was performed, then injected intravenously or intratesticularly consequently with EXs and finally divided in to two subgroups IR-ipsi and IR-contra. The results revealed that testicular IR resulted in functional and structural disorders with reduction in serum testosterone and sperm indices. Interestingly, both testes showed structural histopathological changes and defects in blood testis barrier. There was also an increase in oxidative stress, inflammatory, and apoptosis markers. EXs administration improved the affected parameters probably via modulation of TLR4/MyD88/NF-κB signaling pathway. In conclusion, EXs defended against testicular IR injury. Intratesticular administration had better effects on the ipsilateral testes, whereas Intravenous route had more obvious better effects on the contralateral ones.</p>","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2532195"},"PeriodicalIF":4.0000,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of local versus systemic exosomes administration on both testes in rat model of testicular ischemic reperfusion injury: shedding light on blood testis barrier.\",\"authors\":\"Manar Fouli Gaber Ibrahim, Elshymaa A Abdel-Hakeem, Heba A Shawky, Soha Abdelkawy Abdelwahab, Sahar A Mokhemer\",\"doi\":\"10.1080/21688370.2025.2532195\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Exosomes<sup>,</sup> (EXs) competence in reproductive medicine has been proven, particularly in cases of ischemia reperfusion (IR) injury models. Testicular torsion is a major clinical concern affecting male fertility and results in ischemic reperfusion injury. To the best of our knowledge, up to date, comparative studies using different routes of EXs application have not been investigated. Thus, we aimed to investigate the effect of local versus systemic EXs administration on both testes; ipsilaterally and contralaterally and to explore the involved underlying mechanisms. Rats were allocated into four groups; the control, the ischemia reperfusion group (IR group) in which unilateral testicular IR was performed, then rats were subdivided equally into two subgroups; IR-ipsi group; in which the ipsilateral testes were investigated and the IR-contra group in which the contralateral testes were examined. Group III and group IV in which unilateral IR was performed, then injected intravenously or intratesticularly consequently with EXs and finally divided in to two subgroups IR-ipsi and IR-contra. The results revealed that testicular IR resulted in functional and structural disorders with reduction in serum testosterone and sperm indices. Interestingly, both testes showed structural histopathological changes and defects in blood testis barrier. There was also an increase in oxidative stress, inflammatory, and apoptosis markers. EXs administration improved the affected parameters probably via modulation of TLR4/MyD88/NF-κB signaling pathway. In conclusion, EXs defended against testicular IR injury. Intratesticular administration had better effects on the ipsilateral testes, whereas Intravenous route had more obvious better effects on the contralateral ones.</p>\",\"PeriodicalId\":23469,\"journal\":{\"name\":\"Tissue Barriers\",\"volume\":\" \",\"pages\":\"2532195\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-07-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tissue Barriers\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/21688370.2025.2532195\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue Barriers","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21688370.2025.2532195","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Effect of local versus systemic exosomes administration on both testes in rat model of testicular ischemic reperfusion injury: shedding light on blood testis barrier.
Exosomes, (EXs) competence in reproductive medicine has been proven, particularly in cases of ischemia reperfusion (IR) injury models. Testicular torsion is a major clinical concern affecting male fertility and results in ischemic reperfusion injury. To the best of our knowledge, up to date, comparative studies using different routes of EXs application have not been investigated. Thus, we aimed to investigate the effect of local versus systemic EXs administration on both testes; ipsilaterally and contralaterally and to explore the involved underlying mechanisms. Rats were allocated into four groups; the control, the ischemia reperfusion group (IR group) in which unilateral testicular IR was performed, then rats were subdivided equally into two subgroups; IR-ipsi group; in which the ipsilateral testes were investigated and the IR-contra group in which the contralateral testes were examined. Group III and group IV in which unilateral IR was performed, then injected intravenously or intratesticularly consequently with EXs and finally divided in to two subgroups IR-ipsi and IR-contra. The results revealed that testicular IR resulted in functional and structural disorders with reduction in serum testosterone and sperm indices. Interestingly, both testes showed structural histopathological changes and defects in blood testis barrier. There was also an increase in oxidative stress, inflammatory, and apoptosis markers. EXs administration improved the affected parameters probably via modulation of TLR4/MyD88/NF-κB signaling pathway. In conclusion, EXs defended against testicular IR injury. Intratesticular administration had better effects on the ipsilateral testes, whereas Intravenous route had more obvious better effects on the contralateral ones.
期刊介绍:
Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.