Transcription factor ZNF207 drives aerobic glycolysis and facilitates malignancy progression in hepatocellular carcinoma

Zinc-finger protein 207 (ZNF207), a prominent member of the zinc finger protein family, exhibits consistent upregulation in various cancer types, including hepatocellular carcinoma (HCC). Unfortunately, the specific oncogenic mechanism of ZNF207 in HCC remains unknown. Our research involved a comprehensive approach, utilizing bioinformatics analysis alongside cell functional experiments, dual-luciferase reporter gene assays, ChIP and qPCR investigations, as well as in vivo studies involving nude mice models for subcutaneous tumor transplantation and tail vein lung metastasis, to delve into the molecular mechanisms underlying its oncogenic properties. The study revealed ZNF207's overexpression in HCC tissues, its correlation with diminished overall survival, and the independent prognostic significance of its expression level in HCC. Furthermore, overexpression of ZNF207 was shown to enhance cell viability, proliferation, and invasive capabilities in HCC cells. Mechanistic analyses pointed towards the modulation of ENO1 and GAPDH transcription by ZNF207, fostering aerobic glycolysis and malignant progression. Subsequent in vivo experiments validated that ZNF207 silencing could impede the growth of subcutaneous tumors and lung metastatic nodules in nude mice, consequently extending their survival. More importantly, Pinosylvin exerts its anti-tumor effects by specifically targeting ZNF207, leading to downregulation of GAPDH and ENO1 expression, inhibition of aerobic glycolysis, and suppression of HCC progression in vitro. In conclusion, these observations highlight ZNF207 as a pivotal oncogene in HCC, with potential for therapeutic targeting in patients with this malignancy.