一种用于测定蔗糖的新型改良型酿酒酵母-蔗糖酶杂交生物传感器

IF 2.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Electroanalysis Pub Date : 2025-07-22 DOI:10.1002/elan.70006

Vural Efe, Erol Akyilmaz

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引用次数: 0

摘要

建立了一种基于蔗糖酶-酿酒酵母杂交体系的生物传感器系统，用于蔗糖分子的测定。在0.1 ~ 5.0µM线性范围内测定蔗糖，在0.5 V电位下，检出限为0.08µM，定量限为0.25µM。通过重复性实验，平均值（）、标准差（±S.D.）和变异系数（% V.K.）分别为1.81µM、±0.09µM和4.93%。研究了底物特异性、干扰效应和储存稳定性。测定了果汁样品中的蔗糖含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Novel Modified Invertase-Saccharomyces Cerevisiae Hybrid Biosensor for the Determination of Sucrose

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A Novel Modified Invertase-Saccharomyces Cerevisiae Hybrid Biosensor for the Determination of Sucrose

A biosensor system based on invertase-Saccharomyces cerevisiae hybrid system has been developed for the determination of sucrose molecule. For the determination of sucrose in the linear determination range of 0.1–5.0 µM, at 0.5 V potential, the limit of detection (LOD) was found to be 0.08 µM and the limit of quantification (LOQ) was 0.25 µM. With repeatability experiments, the mean value (), standard deviation (±S.D.), and % coefficient of variation (% V.K.) were found to be 1.81 µM, ±0.09 µM, and 4.93%, respectively. Substrate specificity, interference effect, and storage stability were investigated. Sucrose was determined in fruit juice samples.

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来源期刊

Electroanalysis 化学-电化学

CiteScore

6.00

自引率

3.30%

发文量

222

审稿时长

2.4 months

期刊介绍： Electroanalysis is an international, peer-reviewed journal covering all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with new electrochemical sensors and biosensors, nanobioelectronics devices, analytical voltammetry, potentiometry, new electrochemical detection schemes based on novel nanomaterials, fuel cells and biofuel cells, and important practical applications. Serving as a vital communication link between the research labs and the field, Electroanalysis helps you to quickly adapt the latest innovations into practical clinical, environmental, food analysis, industrial and energy-related applications. Electroanalysis provides the most comprehensive coverage of the field and is the number one source for information on electroanalytical chemistry, electrochemical sensors and biosensors and fuel/biofuel cells.