海鱼病原体海链菌中铁载体生物合成的遗传和生化研究。

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-07-19 DOI:10.1038/s41598-025-08217-6

M Pilar Escribano, Lucía Ageitos, Miguel Balado, Larissa Buedenbender, Jaime Rodríguez, Carlos Jiménez, Beatriz Magariños, Manuel L Lemos

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引用次数: 0

摘要

铁载体的产生是细菌的关键适应性特征，特别是在海洋生态系统和宿主组织等铁有限的环境中。在本研究中，我们通过基因组分析和tencd失活突变体确定了tenABECDC2D2hp1-4基因簇在tenacbaculum maritimum中负责铁载体的生物合成。该簇与链霉菌中的去铁胺生物合成系统高度相似，具有独特的tenCD复制/融合，对铁载体的形成至关重要。此外，辅助基因（hp1-4）编码硝基还原酶和n -乙酰转移酶等功能，可能有助于铁载体多样化。LC/MS分析表明，海苔菌培养物可产生20个两亲性的酰化地铁胺样铁载体。这些发现为海洋病原体在铁获取中的遗传和代谢多样性提供了新的见解。

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Genetic and biochemical insights into siderophore biosynthesis in the marine fish pathogen Tenacibaculum maritimum.

Siderophore production is a key fitness trait for bacteria, particularly in iron-limited environments such as marine ecosystems and host tissues. In this study, we identify the tenABECDC2D2hp1-4 gene cluster as responsible for siderophore biosynthesis in Tenacibaculum maritimum, confirmed through genome analysis and a tenCD-inactivated mutant. This cluster, highly similar to the desferrioxamine biosynthesis system in Streptomyces coelicolor, features a unique tenCD duplication/fusion, essential for siderophore formation. Additionally, accessory genes (hp1-4) encode functions such as nitroreductase and N-acetyltransferase, likely contributing to siderophore diversification. LC/MS analysis of T. maritimum cultures revealed the production of 20 amphiphilic, acylated desferrioxamine-like siderophores. These findings provide new insights into the genetic and metabolic versatility of marine pathogens in iron acquisition.

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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