尸体AMD样本的分泌性自噬和上皮到间质转化：疾病进展的新途径

IF 3 3区医学 Q1 OPHTHALMOLOGY

Acta Ophthalmologica Pub Date : 2025-07-19 DOI:10.1111/aos.17558

Iswariyaraja Sridevi Gurubaran, Ali Koskela, Hanna Heloterä, Kai Kaarniranta

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引用次数: 0

摘要

目的：探讨不同形式老年性黄斑变性（AMD）的人眼黄斑视网膜色素上皮（RPE）中分泌性自噬和上皮间质转化（EMT）的存在。方法：采用免疫组织化学方法对干性、湿性AMD的人尸体黄斑样本以及年龄匹配的对照组进行分析。在RPE细胞中评估分泌性自噬、EMT和炎症标志物。结果：与对照组相比，在AMD样本的RPE中检测到与分泌性自噬和EMT相关的蛋白表达增加。这些变化在干型和湿型AMD中都可以观察到。结论：amd患者黄斑RPE的分泌性自噬和EMT升高。这些观察结果为AMD的进展和潜在的治疗方法提供了新的见解。

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Secretory autophagy and epithelial-to-mesenchymal transition in cadaveric AMD samples: Novel pathways in disease progression.

Purpose: To examine the presence of secretory autophagy and epithelial-mesenchymal transition (EMT) in the macular retinal pigment epithelium (RPE) of human cadaver eyes with different forms of age-related macular degeneration (AMD).

Methods: Human cadaver macula samples representing dry and wet AMD, as well as age-matched controls, were analyzed using immunohistochemistry. Markers of secretory autophagy, EMT, and inflammation were evaluated in RPE cells.

Results: Increased expression of proteins associated with secretory autophagy and EMT was detected in the RPE of AMD samples compared to controls. These changes were observed in both dry and wet AMD forms.

Conclusion: Secretory autophagy and EMT are elevated in the macular RPE of AMD-affected eyes. These observations offer novel insight into AMD progression and potential therapeutic approaches.

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.