Xinrong Zou, Meiling Pang, Jun He, Zhuxi Kang, Gulixiayida Wumaier, Shenglai Xue and Lingli Yuan
{"title":"用于炎性细胞、斑马鱼和小鼠血液样本中脂多糖诱导的H2S检测和成像的有效荧光传感器","authors":"Xinrong Zou, Meiling Pang, Jun He, Zhuxi Kang, Gulixiayida Wumaier, Shenglai Xue and Lingli Yuan","doi":"10.1039/D5RA03905C","DOIUrl":null,"url":null,"abstract":"<p >In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced H<small><sub>2</sub></small>S detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the <em>o</em>-aldehyde group (<em>o</em>-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards H<small><sub>2</sub></small>S, which can realize fluorescence “turn-on” detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward H<small><sub>2</sub></small>S. Additionally, the excellent detection limit (LOD) for H<small><sub>2</sub></small>S is as low as 25.6 nM. Impressively, this <em>o</em>-CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for H<small><sub>2</sub></small>S detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.</p>","PeriodicalId":102,"journal":{"name":"RSC Advances","volume":" 32","pages":" 25863-25871"},"PeriodicalIF":4.6000,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra03905c?page=search","citationCount":"0","resultStr":"{\"title\":\"An effective fluorescent sensor for lipopolysaccharide-induced H2S detection and imaging in inflammatory cells, zebrafish, and mouse blood samples†\",\"authors\":\"Xinrong Zou, Meiling Pang, Jun He, Zhuxi Kang, Gulixiayida Wumaier, Shenglai Xue and Lingli Yuan\",\"doi\":\"10.1039/D5RA03905C\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced H<small><sub>2</sub></small>S detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the <em>o</em>-aldehyde group (<em>o</em>-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards H<small><sub>2</sub></small>S, which can realize fluorescence “turn-on” detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward H<small><sub>2</sub></small>S. Additionally, the excellent detection limit (LOD) for H<small><sub>2</sub></small>S is as low as 25.6 nM. Impressively, this <em>o</em>-CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for H<small><sub>2</sub></small>S detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.</p>\",\"PeriodicalId\":102,\"journal\":{\"name\":\"RSC Advances\",\"volume\":\" 32\",\"pages\":\" 25863-25871\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-07-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra03905c?page=search\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"RSC Advances\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra03905c\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC Advances","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra03905c","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
An effective fluorescent sensor for lipopolysaccharide-induced H2S detection and imaging in inflammatory cells, zebrafish, and mouse blood samples†
In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced H2S detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the o-aldehyde group (o-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards H2S, which can realize fluorescence “turn-on” detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward H2S. Additionally, the excellent detection limit (LOD) for H2S is as low as 25.6 nM. Impressively, this o-CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for H2S detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.
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An international, peer-reviewed journal covering all of the chemical sciences, including multidisciplinary and emerging areas. RSC Advances is a gold open access journal allowing researchers free access to research articles, and offering an affordable open access publishing option for authors around the world.
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