Eduardo Carlos Rudell , Luan Cutti , Guilherme Menegol Turra , Paula Sinigaglia Angonese , Vinicius Ferrari Tasca , Othon Dias dos Santos , Eric Patterson , Aldo Merotto
{"title":"巴西棘球藻抗als抑制剂机制的变异和空间分布","authors":"Eduardo Carlos Rudell , Luan Cutti , Guilherme Menegol Turra , Paula Sinigaglia Angonese , Vinicius Ferrari Tasca , Othon Dias dos Santos , Eric Patterson , Aldo Merotto","doi":"10.1016/j.plaphy.2025.110237","DOIUrl":null,"url":null,"abstract":"<div><div>Barnyardgrass (<em>Echinochloa crus-galli</em> (L.) P. Beauv.) is a hexaploid weed, commonly found in rice fields. The field-level frequencies of the herbicide resistance mechanisms present in barnyardgrass remain unknown. This study developed and compared molecular marker assays for detecting mutations in the <em>ALS</em> genes, analyzing their frequency and spatial distribution in Southern Brazil rice fields. The <em>ALS</em> gene of 52 accessions was sequenced to identify mutations associated with resistance. Single Nucleotide-Amplified Polymorphism (SNAP) and PCR Allele Competitive Extension (PACE®) markers were developed for detecting ALS mutations: A122T, A205N, W574L, and S653N. A total of 233 accessions that survived imidazolinone application were collected. A greenhouse assay identified 195 and 84 accessions resistant to imazapyr + imazapic and penoxsulam, respectively. Molecular assays detected 188 resistant accessions, with W574L, S653N, A122T, and A205N mutations present in 43 %, 29 %, 17 %, and 5 % of resistant samples, respectively. 6 % of accessions carried mutations in two positions, while six resistant accessions lacked any mutation. Efficiency of SNAP and PACE methods was 90 % and 82 %, respectively. Discrepancies between methods were resolved using Nanopore sequencing. The detection threshold was one resistant per 25 susceptible DNA samples and one per 10 using leaf discs in SNAP. All mutations were distributed geographically, with the frequency of W574L increasing from 40 % in 2017/2018 to 47 % in the 2022/2023 season. ALS resistance was detected in 80 % of the accessions. Epidemiological studies, like this, that track resistance mechanisms, including the occurrence, distribution, and variability of mutations, are crucial for improving weed control recommendations.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110237"},"PeriodicalIF":5.7000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Variability and spatial distribution of ALS-inhibitor resistance mechanisms in Brazilian Echinochloa crus-galli\",\"authors\":\"Eduardo Carlos Rudell , Luan Cutti , Guilherme Menegol Turra , Paula Sinigaglia Angonese , Vinicius Ferrari Tasca , Othon Dias dos Santos , Eric Patterson , Aldo Merotto\",\"doi\":\"10.1016/j.plaphy.2025.110237\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Barnyardgrass (<em>Echinochloa crus-galli</em> (L.) P. Beauv.) is a hexaploid weed, commonly found in rice fields. The field-level frequencies of the herbicide resistance mechanisms present in barnyardgrass remain unknown. This study developed and compared molecular marker assays for detecting mutations in the <em>ALS</em> genes, analyzing their frequency and spatial distribution in Southern Brazil rice fields. The <em>ALS</em> gene of 52 accessions was sequenced to identify mutations associated with resistance. Single Nucleotide-Amplified Polymorphism (SNAP) and PCR Allele Competitive Extension (PACE®) markers were developed for detecting ALS mutations: A122T, A205N, W574L, and S653N. A total of 233 accessions that survived imidazolinone application were collected. A greenhouse assay identified 195 and 84 accessions resistant to imazapyr + imazapic and penoxsulam, respectively. Molecular assays detected 188 resistant accessions, with W574L, S653N, A122T, and A205N mutations present in 43 %, 29 %, 17 %, and 5 % of resistant samples, respectively. 6 % of accessions carried mutations in two positions, while six resistant accessions lacked any mutation. Efficiency of SNAP and PACE methods was 90 % and 82 %, respectively. Discrepancies between methods were resolved using Nanopore sequencing. The detection threshold was one resistant per 25 susceptible DNA samples and one per 10 using leaf discs in SNAP. All mutations were distributed geographically, with the frequency of W574L increasing from 40 % in 2017/2018 to 47 % in the 2022/2023 season. ALS resistance was detected in 80 % of the accessions. Epidemiological studies, like this, that track resistance mechanisms, including the occurrence, distribution, and variability of mutations, are crucial for improving weed control recommendations.</div></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"228 \",\"pages\":\"Article 110237\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S098194282500765X\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S098194282500765X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Variability and spatial distribution of ALS-inhibitor resistance mechanisms in Brazilian Echinochloa crus-galli
Barnyardgrass (Echinochloa crus-galli (L.) P. Beauv.) is a hexaploid weed, commonly found in rice fields. The field-level frequencies of the herbicide resistance mechanisms present in barnyardgrass remain unknown. This study developed and compared molecular marker assays for detecting mutations in the ALS genes, analyzing their frequency and spatial distribution in Southern Brazil rice fields. The ALS gene of 52 accessions was sequenced to identify mutations associated with resistance. Single Nucleotide-Amplified Polymorphism (SNAP) and PCR Allele Competitive Extension (PACE®) markers were developed for detecting ALS mutations: A122T, A205N, W574L, and S653N. A total of 233 accessions that survived imidazolinone application were collected. A greenhouse assay identified 195 and 84 accessions resistant to imazapyr + imazapic and penoxsulam, respectively. Molecular assays detected 188 resistant accessions, with W574L, S653N, A122T, and A205N mutations present in 43 %, 29 %, 17 %, and 5 % of resistant samples, respectively. 6 % of accessions carried mutations in two positions, while six resistant accessions lacked any mutation. Efficiency of SNAP and PACE methods was 90 % and 82 %, respectively. Discrepancies between methods were resolved using Nanopore sequencing. The detection threshold was one resistant per 25 susceptible DNA samples and one per 10 using leaf discs in SNAP. All mutations were distributed geographically, with the frequency of W574L increasing from 40 % in 2017/2018 to 47 % in the 2022/2023 season. ALS resistance was detected in 80 % of the accessions. Epidemiological studies, like this, that track resistance mechanisms, including the occurrence, distribution, and variability of mutations, are crucial for improving weed control recommendations.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.