Peng Zheng , Yan Li , Youpeng Cheng , Jing Wang , Yixuan Wang , Yang Mu , Jinyou Shen
{"title":"通过调节生物膜分层和微生物空间相互作用,通过反扩散生物膜中的短程吡啶- n转化增强挥发性吡啶生物降解的机理","authors":"Peng Zheng , Yan Li , Youpeng Cheng , Jing Wang , Yixuan Wang , Yang Mu , Jinyou Shen","doi":"10.1016/j.biortech.2025.133010","DOIUrl":null,"url":null,"abstract":"<div><div>Air pollution and nitrogen contamination residues remain challenges in the conventional biological treatment of industrial wastewater containing volatile organic compounds. In this study, counter-diffusion biofilms were integrated with shortcut nitrification–denitrification technology to enhance pyridine biodegradation through shortcut pyridine-N transformation (EPB-SPNT), with emphasis on biofilm stratification and microbial spatial interactions. Results showed that the removal efficiency of pyridine and total nitrogen reached 100 % and 91.24 ± 0.75 %, respectively. Fluorescence in situ hybridization and metagenomic analysis revealed that aerobic pyridine-degrading bacteria (APDB, <em>Alicycliphilus</em>) and ammonia-oxidizing bacteria (AOB, <em>Nitrosomonas</em>) were located in the aerobic layer, while anoxic pyridine degrading-denitrifying bacteria (APD-DB, <em>Paracoccus</em>) were enriched in the anoxic layer. Biofilm stability was mainly attributed to the lower hydrophilicity of protein secondary structure. The EPB-SPNT process was driven by the spatial cooperation among APDB, AOB, and APD-DB. These findings demonstrate the feasibility of implementing the EPB-SPNT in counter-diffusion biofilms through the regulation of microbial stratification and interactions.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"436 ","pages":"Article 133010"},"PeriodicalIF":9.7000,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanistic insights into enhanced volatile pyridine biodegradation through shortcut pyridine-N transformation in counter-diffusion biofilms by regulating biofilm stratification and microbial spatial interactions\",\"authors\":\"Peng Zheng , Yan Li , Youpeng Cheng , Jing Wang , Yixuan Wang , Yang Mu , Jinyou Shen\",\"doi\":\"10.1016/j.biortech.2025.133010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Air pollution and nitrogen contamination residues remain challenges in the conventional biological treatment of industrial wastewater containing volatile organic compounds. In this study, counter-diffusion biofilms were integrated with shortcut nitrification–denitrification technology to enhance pyridine biodegradation through shortcut pyridine-N transformation (EPB-SPNT), with emphasis on biofilm stratification and microbial spatial interactions. Results showed that the removal efficiency of pyridine and total nitrogen reached 100 % and 91.24 ± 0.75 %, respectively. Fluorescence in situ hybridization and metagenomic analysis revealed that aerobic pyridine-degrading bacteria (APDB, <em>Alicycliphilus</em>) and ammonia-oxidizing bacteria (AOB, <em>Nitrosomonas</em>) were located in the aerobic layer, while anoxic pyridine degrading-denitrifying bacteria (APD-DB, <em>Paracoccus</em>) were enriched in the anoxic layer. Biofilm stability was mainly attributed to the lower hydrophilicity of protein secondary structure. The EPB-SPNT process was driven by the spatial cooperation among APDB, AOB, and APD-DB. These findings demonstrate the feasibility of implementing the EPB-SPNT in counter-diffusion biofilms through the regulation of microbial stratification and interactions.</div></div>\",\"PeriodicalId\":258,\"journal\":{\"name\":\"Bioresource Technology\",\"volume\":\"436 \",\"pages\":\"Article 133010\"},\"PeriodicalIF\":9.7000,\"publicationDate\":\"2025-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresource Technology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0960852425009769\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960852425009769","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
Mechanistic insights into enhanced volatile pyridine biodegradation through shortcut pyridine-N transformation in counter-diffusion biofilms by regulating biofilm stratification and microbial spatial interactions
Air pollution and nitrogen contamination residues remain challenges in the conventional biological treatment of industrial wastewater containing volatile organic compounds. In this study, counter-diffusion biofilms were integrated with shortcut nitrification–denitrification technology to enhance pyridine biodegradation through shortcut pyridine-N transformation (EPB-SPNT), with emphasis on biofilm stratification and microbial spatial interactions. Results showed that the removal efficiency of pyridine and total nitrogen reached 100 % and 91.24 ± 0.75 %, respectively. Fluorescence in situ hybridization and metagenomic analysis revealed that aerobic pyridine-degrading bacteria (APDB, Alicycliphilus) and ammonia-oxidizing bacteria (AOB, Nitrosomonas) were located in the aerobic layer, while anoxic pyridine degrading-denitrifying bacteria (APD-DB, Paracoccus) were enriched in the anoxic layer. Biofilm stability was mainly attributed to the lower hydrophilicity of protein secondary structure. The EPB-SPNT process was driven by the spatial cooperation among APDB, AOB, and APD-DB. These findings demonstrate the feasibility of implementing the EPB-SPNT in counter-diffusion biofilms through the regulation of microbial stratification and interactions.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.