深共熔溶剂对漆酶活性和热稳定性的影响

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-07-16 DOI:10.1016/j.biortech.2025.132992

Li Yang , Jia Chen , Kangshun Xie , Kaiwen He , Wenyang Sheng , Mi Zhou , Guangyang Jiang , Yongqiang Tian

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引用次数: 0

摘要

对18种深度共熔溶剂（DES）体系进行了系统评价，以优化漆酶生物催化。5种氢键受体（HBAs）显著提高了酶的活性和热稳定性。胆碱磷酸二氢(ChDHP)-甘油DES的活性提高最高（198%）。此外，50%甜菜碱-山梨糖醇（BS）溶液在70°c下1小时后仍保持92%的活性，比缓冲液高55倍，解决了关键的热稳定性挑战。荧光光谱（FS）和分子对接表明，DES溶液通过与催化中心组氨酸（His）和苯丙氨酸（Phe）残基形成氢键来稳定漆酶的天然结构。bs -漆酶预处理可使酒糟（DG）和杨木（PW）的葡萄糖转化率分别提高30%和54%。这些发现证明了DES是一种可持续的、低成本的助溶剂，可以提高酶的效率，为hba -酶的相互作用提供分子见解，指导循环生物经济的生物催化系统设计。

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Effects of deep eutectic solvent on laccase activity and thermal stability

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Effects of deep eutectic solvent on laccase activity and thermal stability

Eighteen deep eutectic solvent (DES) systems were systematically evaluated to optimize laccase biocatalysis. Five hydrogen bond acceptors (HBAs) significantly enhanced both enzyme activity and thermal stability. The choline dihydrogen phosphate (ChDHP)-glycerol DES yielded the highest activity increase (198 %). Moreover, a 50 % betaine-sorbitol (BS) solution retained 92 % activity after 1 h at 70 °C—55-fold higher than buffer—addressing a key thermal stability challenge. Fluorescence spectroscopy (FS) and molecular docking revealed that DES solutions stabilized laccase’s native structure by forming hydrogen bonds with catalytic center histidine (His) and phenylalanine (Phe) residues. BS-laccase pretreatment increased glucose conversion by 30 % in distillers’ grains (DG) and 54 % in poplar wood (PW). These findings establish DES as a sustainable, low-cost co-solvent that boosts enzymatic efficiency, offering molecular insights into HBA-enzyme interactions to guide biocatalytic system design for circular bioeconomy.

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.